Yan Wenjie, Liu Jiao, Seng Samphal, Zhou Bin, Ding Kuke. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. Acta Oceanologica Sinica, 2019, 38(10): 111-115. doi: 10.1007/s13131-019-1429-z
Citation: Yan Wenjie, Liu Jiao, Seng Samphal, Zhou Bin, Ding Kuke. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. Acta Oceanologica Sinica, 2019, 38(10): 111-115. doi: 10.1007/s13131-019-1429-z

Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

doi: 10.1007/s13131-019-1429-z
  • Received Date: 2018-05-20
  • The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.
  • 加载中
  • [1] Costanza R, d’Arge R, De Groot R, et al. 1997. The value of the world’s ecosystem services and natural capital. Nature, 387(6630): 253–260, doi:  10.1038/387253a0
    [2] Den Hartog C. 1970. The Seagrasses of the World. Amsterdam: North Holland Publishing Company, 265
    [3] Dixon D P, Lapthorn A, Edwards R. 2002. Plant glutathione transferases. Genome Biology, 3(3): eviews3004.1
    [4] Edwards R, Dixon D P, Walbot V. 2000. Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends in Plant Science, 5(5): 193–198, doi:  10.1016/S1360-1385(00)01601-0
    [5] Fourqurean J W, Duarte C M, Kennedy H, et al. 2012. Seagrass ecosystems as a globally significant carbon stock. Nature Geoscience, 5(7): 505–509, doi:  10.1038/ngeo1477
    [6] Green E P, Short F T. 2003. World Atlas of Seagrasses. Berkeley: University of California Press, 185–192
    [7] Hemminga M A, Duarte C M. 2000. Seagrass Ecology. Cambridge: Cambridge University Press, 298
    [8] Larkum A W D, Orth R J, Duarte C M. 2006. Seagrasses: Biology, Ecology and Conservation. Netherlands: Springer, 25–50
    [9] Les D H, Cleland M A, Waycott M. 1997. Phylogenetic studies in Alismatidae, Ⅱ: evolution of marine angiosperms (seagrasses) and hydrophily. Systematic Botany, 22(3): 443–463, doi:  10.2307/2419820
    [10] Logue J, Tiku P, Cossins A R. 1995. Heat injury and resistance adaptation in fish. Journal of Thermal Biology, 20(1–2): 191–197
    [11] Mannervik B, Danielson U H, Ketterer B. 1988. Glutathione transferase-structure and catalytic activity. Critical Reviews in Biochemistry, 23(2): 283–337
    [12] Martin J R, Specht J E, Williams J H, et al. 1978. Temperature tolerance in soybeans: I. Evaluation of a technique for assessing cellular membrane thermostability. Crop Science, 19(1): 75–81
    [13] Olsen J L, Rouzé P, Verhelst B, et al. 2016. The genome of the seagrass Zostera marina reveals angiosperm adaptation to the sea. Nature, 530(7590): 331–335, doi:  10.1038/nature16548
    [14] Rushmore T H, Pickett C B. 1993. Glutathione S-transferases, structure, regulation, and therapeutic implications. The Journal of Biological Chemistry, 268(16): 11475–11478
    [15] Sheehan D, Meade G, Foley V M, et al. 2001. Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily. Biochemical Journal, 360(1): 1–16, doi:  10.1042/bj3600001
    [16] Wilce M C J, Parker M W. 1994. Structure and function of glutathione S-transferases. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1205(1): 1–18, doi:  10.1016/0167-4838(94)90086-8
    [17] Wright J P, Jones C G. 2006. The concept of organisms as ecosystem engineers ten years on: progress, limitations, and challenges. BioScience, 56(3): 203–209, doi:  10.1641/0006-3568(2006)056[0203:TCOOAE]2.0.CO;2
    [18] Yan Fei, Yang Wenkui, Li Xinyang, et al. 2008. A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity. Biochimica et Biophysica Acta (BBA)-General Subjects, 1780(6): 869–872, doi:  10.1016/j.bbagen.2008.03.003
    [19] Zhang Yi, Lu Tiegang. 2011. The research of Reactive Oxygen Species (ROS) in plants. Current Biotechnology (in Chinese), 1(4): 242–248
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(216) PDF downloads(177) Cited by()

Related
Proportional views

Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

doi: 10.1007/s13131-019-1429-z

Abstract: The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.

Yan Wenjie, Liu Jiao, Seng Samphal, Zhou Bin, Ding Kuke. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. Acta Oceanologica Sinica, 2019, 38(10): 111-115. doi: 10.1007/s13131-019-1429-z
Citation: Yan Wenjie, Liu Jiao, Seng Samphal, Zhou Bin, Ding Kuke. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. Acta Oceanologica Sinica, 2019, 38(10): 111-115. doi: 10.1007/s13131-019-1429-z
Reference (19)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return