Home > 2017, 36(10) > Stocking density effects on growth and stress response of juvenile turbot (Scophthalmus maximus) reared in land-based recirculating aquaculture system

Citation: LIU Baoliang, JIA Rui, ZHAO Kuifeng, WANG Guowen, LEI Jilin and HUANG Bin, . Stocking density effects on growth and stress response of juvenile turbot (Scophthalmus maximus) reared in land-based recirculating aquaculture system. ACTA OCEANOLOGICA SINICA, 2017, 36(10): 31-38. doi: 10.1007/s13131-017-0976-4

2017, 36(10): 31-38. doi: 10.1007/s13131-017-0976-4

Stocking density effects on growth and stress response of juvenile turbot (Scophthalmus maximus) reared in land-based recirculating aquaculture system

1.  Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
2.  Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture
3.  Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
4.  Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China
5.  Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China,Shandong Oriental Ocean Sci-Tech Co., Ltd, Yantai 264000, China,Shandong Oriental Ocean Sci-Tech Co., Ltd, Yantai 264000, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
6.  Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China and Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences

Received Date: 2016-05-24

Stocking density is widely recognized as a critical factor in aquaculture and a potential source of long-term stress. The influence of stocking density on growth and stress response of juvenile turbot (Scophthalmus maximus,~3-75 g, initial to final weight) was examined in fish held under low (LD,~0.21-5.31 kg/m2, initial to final density), medium (MD,~0.42-10.81 kg/m2) and high stocking density (HD,~0.63-14.27 kg/m2) for 120 days in a recirculating aquaculture system (RAS). In this trial, the growth curve for weight of juvenile turbot in RAS, all fitted by the Schnute model. No significant difference was found in growth performance among the three densities until at the final sampling (Day 120). The final weight and body weight increase (BWI) in the HD group were significantly lower than in other groups (P<0.05, weight:(75.83±2.49) g, (75.39±2.08) g, (65.72±2.86) g and BWI:(2 436.12±28.10)%, (2 421.29±4.64)%, (2 097.88±20.99)% in LD, MD and HD groups, respectively). Similarly, the specific growth rate (SGR), feed conversion ratio (FCR) and coefficient of variation for weight (CVw) were adversely affected by high stocking density (P<0.05). However, there was no difference in survival and Fulton's condition factor (K) of turbot among the different groups. Physiological analyses demonstrated a clear increase in the plasma cortisol level and an obvious decrease in growth hormone (GH) concentration in the HD group on Day 120 (P<0.05). There was no significant effect of stocking density on plasma glucose, Cl- and protein levels. All these findings would provide a reference for selecting the optimal stocking density of juvenile turbot in RAS.

Key words: growth performance , recirculating aquaculture system , Scophthalmus maximus , stress physiology , stocking density

养殖密度是水产养殖过程中的重要影响因素,亦是养殖动物潜在的慢性应激源。大菱鲆幼鱼(Scophthalmus maximus,~3-75 g,初始至末重)分别以低(LD,~0.21-5.31 kg/m2,initial to final density),中(MD,~0.42-10.81 kg/m2),高(HD,~0.63-14.27 kg/m2)三种养殖密度放养于循环水养殖系统中(recirculating aquaculture system,RAS)120天,以研究养殖密度对其生长和应激反应的影响。实验结果表明,三个养殖密度组中大菱鲆生长曲线均符合Schnute生长模型。在最后一次取样(120天)监测之前不同养殖密度中大菱鲆生长无显著差异。养殖实验末,高密度组中大菱鲆体重与增重率(body weight increase,BWI)均显著低于其它组(P < 0.05,体重:75.83±2.49 g,75.39±2.08 g,65.72±2.86 g;BWI:2436.12±28.10%,2421.29±4.64%,2097.88±20.99%于LD,MD和HD组)。此外高密度均对大菱鲆特定生长率(SGR),饲料转化率(FCR)以及体重差异系数(CV)产生负面影响(P < 0.05)。尽管如此,不同实验组间大菱鲆存活率,肥满度无显著差异。生理参数分析结果表明,在120天时,高密度组大菱鲆血浆中皮质醇含量显著升高,生长激素水平显著下降(P < 0.05),但血糖、Cl-和总蛋白水平未受影响。本研究将为循环水养殖系统中大菱鲆幼鱼适宜放养密度的选取提供理论参考。

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Stocking density effects on growth and stress response of juvenile turbot (Scophthalmus maximus) reared in land-based recirculating aquaculture system

LIU Baoliang, JIA Rui, ZHAO Kuifeng, WANG Guowen, LEI Jilin and HUANG Bin,