Home > Online First > Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

Citation: Wenjie Yan, Jiao Liu, Samphal Seng, Bin Zhou, Kuke Ding. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina. ACTA OCEANOLOGICA SINICA, doi: 10.1007/s13131-019-1429-z

doi: 10.1007/s13131-019-1429-z

Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

1.  Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
2.  Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
3.  Faculty of Fisheries & Aquaculture, Royal University of Agriculture, Phnom Penh 12101, Cambodia
4.  National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
5.  Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, China

Corresponding author: Kuke Ding, shouding@ccmu.edu.cn

Received Date: 2018-05-20
Web Publishing Date: 2019-08-01

Fund Project: Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology, State Oceanic Administration (201704). The Shandong Provincial Natural Science Foundation of China under contract No. ZR2018MD020.

In the text, the response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.

Key words: Zostera marina , antioxidant enzyme , glutathione S-transferase (GST) , temperature , enzyme activity

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Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

Wenjie Yan, Jiao Liu, Samphal Seng, Bin Zhou, Kuke Ding