SARAVANAN R., SUGUMAR V., BEEMA MAHIN M. I.. Heavy metal stress induced hyperglycemia in blue swimmer crab, Portunus pelagicus[J]. Acta Oceanologica Sinica, 2018, 37(5): 47-53. doi: 10.1007/s13131-018-1211-7
Citation: SARAVANAN R., SUGUMAR V., BEEMA MAHIN M. I.. Heavy metal stress induced hyperglycemia in blue swimmer crab, Portunus pelagicus[J]. Acta Oceanologica Sinica, 2018, 37(5): 47-53. doi: 10.1007/s13131-018-1211-7

Heavy metal stress induced hyperglycemia in blue swimmer crab, Portunus pelagicus

doi: 10.1007/s13131-018-1211-7
  • Received Date: 2017-01-11
  • Rev Recd Date: 2017-08-01
  • The study was to find out the effect of cadmium and mercury on levels of hemolymph glucose, crustacean hyperglycemic hormone (CHH) and hepatopancreas glycogen in the blue swimmer crab Portunus pelagicus. The experiments were performed in both intact and eyestalk ablated crabs. Quantification of CHH was done by the indirect ELISA with the aid of primary anti-Carcinus maenas-CHH antibody. Higher glucose concentration was observed on exposure to 8×10-6 of cadmium ((825.6±5.42) μg/mL) and 6×10-6 of mercury ((90.5±6.25) μg/mL) after 48 h and 24 h respectively. Higher level of hemolymph glucose was observed in eyestalk intact crabs on exposure to cadmium and mercury than eyestalk ablated crabs. Decrease in the levels of CHH was observed in both eyestalk intact and ablated crabs on heavy metal exposure. Decline of the hepatopancreas glycogen level was also witnessed with the exposure to heavy metal, which validated its utilization in the production of glucose. Thus this study brings to light, the variations in hemolymph glucose, CHH and hepatopancreas glycogen on heavy metal stress. These carbohydrate metabolites can be used as biomarkers in assessing heavy metal contamination in water bodies.
  • loading
  • Abramowitz A, Hisaw F, Papandrea D. 1944. The occurrence of a diabetogenic factor in the eyestalks of crustaceans. The Biological Bulletin, 86:1-4
    Ahsanullah M, Negilski D S, Mobley M C. 1981. Toxicity of zinc, cadmium and copper to the shrimp Callianassa australiensis:I. Effects of individual metals. Marine Biology, 64(S3):299-304
    Ahsanullah M, Ying W. 1995. Toxic effects of dissolved copper on Penaeus merguiensis and Penaeus monodon. Bulletin of Environmental Contamination and Toxicology, 55(1):81-88
    Bryan G W. 1984. Pollution due to heavy metals and their compounds. In:Kinne O, ed. Marine Ecology:A Comprehensive, Integrated Treatise on Life in Oceans and Coastal Waters. London:John Wiley & Sons Ltd, 1290-1430
    Carroll N V, Longley A, Roe J H. 1956. The determination of glycogen in liver and muscle by use of anthrone reagent. The Journal of Biological Chemistry, 220(2):583-593
    Chang E S, Keller R, Chang S A. 1998. Quantification of crustacean hyperglycemic hormone by ELISA in hemolymph of the lobster, Homarus americanus, following various stresses. General and Comparative Endocrinology, 111(3):359-366
    Chung J S, Zmora N. 2008. Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus—the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress. The FEBS Journal, 275(4):693-704
    Batoy C B, Sarmago J F, Pilapil B C. 1980. Breeding season, sexual maturity and fecundity of blue crab, Portunus pelagicus (L.) in selected coastal waters in Leyte and Vicinity, Philippines. Annals of Tropical Research, 9:157-177
    Dean J M, Vernberg F J. 1965. Effects of temperature acclimation on some aspects of carbohydrate metabolism in decapod crustaceans. The Biological Bulletin, 129:87-94
    Fingerman M, Devi M, Reddy P S, et al. 1996. Impact of heavy metal exposure on the nervous system and endocrine-mediated processes in crustaceans. Zoological Studies, 35(1):1-8
    Fingerman M, Jackson N C, Nagabhushanam R. 1998. Hormonally-regulated functions in crustaceans as biomarkers of environmental pollution. Comparative Biochemistry and Physiology Part C:Pharmacology, Toxicology and Endocrinology, 120(3):343-350
    Govindasamy C, Arulpriya M, Ruban P, et al. 2011. Concentration of heavy metals in seagrasses tissue of the Palk strait, Bay of Bengal. International Journal of Environmental Sciences, 2(1):145-153
    Joel D R, Raj P S S. 1987. Marine crab fisheries around Pulcat. Seafood Expo, 19:16-24
    Júdová J. 2006. Crustacea and heavy metal accumulation. Oecologia Montana, 15:29-37
    Kyomo J. 1999. Distribution and abundance of crustaceans of commercial importance in Tanzania Mainland coastal waters. Bulletin of Marine Science, 65:321-335
    Levenson J, Byrne J H, Eskin A. 1999. Levels of serotonin in the hemolymph of Aplysia are modulated by light/dark cycles and sensitization training. The Journal of Neuroscience:the Official Journal of the Society for Neuroscience, 19(18):8094-8103
    Li Li, Tian Xiangli, Yu Xiao, et al. 2016. Effects of acute and chronic heavy metal (Cu, Cd, and Zn) exposure on sea cucumbers (Apostichopus japonicus). BioMed Research International, 20167:453269
    Lorenzon S, Edomi P, Giulianini P G, et al. 2004. Variation of crustacean hyperglycemic hormone (cHH) level in the eyestalk and haemolymph of the shrimp Palaemon elegans following stress. The Journal of Experimental Biology, 207(Pt 24):4205-4213
    Lorenzon S, Edomi P, Giulianini P G, et al. 2005. Role of biogenic amines and cHH in the crustacean hyperglycemic stress response. The Journal of Experimental Biology, 208(Pt 17):3341-3347
    Lorenzon S, Francese M, Ferrero E A. 2000. Heavy metal toxicity and differential effects on the hyperglycemic stress response in the shrimp, Palaemon elegans. Archives of Environmental Contamination and Toxicology, 39(2):167-176
    Machale P R, Khan A K, Sarojini R, et al. 1989. Copper and cadmium induced changes in blood sugar level of the crab Barytelphusa cunicularis. Uttar Pradesh Journal of Zoology, 9(1):113-115
    Madsen T, Shine R. 1992. Determinants of reproductive success in female adders, Vipera berus. Oecologia, 92(1):40-47
    McGee E A, Hsu S Y, Kaipia A, et al. 1998. Cell death and survival during ovarian follicle development. Molecular and Cellular Endocrinology, 140(1-2):15-18
    McPherson R, Brown K. 2001. The bioaccumulation of cadmium by the blue swimmer crab Portunus pelagicus L.. Science of the Total Environment, 279(1-3):223-230
    Mgaya Y D, Muruke M H S, Chande A I, et al. 1999. Evaluation of crustacean resources in Bagamoyo District. In:Howell K M, Semesi A K, eds. Coastal Resources of Bagamoyo District Tanzania. Proceedings of a workshop on coastal resources of Bagamoyo, 18-19 December 1997, Bagamoyo. Faculty of Science, University of Dar es Salaam, 55-63
    Migliore L, De Nicola Giudici M. 1990. Toxicity of heavy metals to Asellus aquaticus (L.) (Crustacea, Isopoda). Hydrobiologia, 203(3):155-164
    Moreno P A R, Medesani D A, Rodrıguez E M. 2003. Inhibition of molting by cadmium in the crab Chasmagnathus granulata (Decapoda Brachyura). Aquatic Toxicology, 64(2):155-164
    Nagabhushanam R, Kulkarni G K. 1981. Freshwater palaemonid prawn, Macrobrachium kistnensis (Tiwari)—Effect of heavy metal pollutants. Proceedings of the Indian National Science Academy. Part B. Biological Sciences, 47B:380-386
    Naqvi S M, Howell R D. 1993. Toxicity of cadmium and lead to juvenile red swamp crayfish, Procambarus clarkii, and effects on fecundity of adults. Bulletin of Environmental Contamination and Toxicology, 51(2):303-308
    Nithya C, Karutha Pandian S. 2010. Isolation of heterotrophic bacteria from Palk Bay sediments showing heavy metal tolerance and antibiotic production. Microbiological Research, 165(7):578-593
    Palanichamy S, Rajendran A. 2000. Heavy metal concentrations in seawater and sediments of Gulf of Mannar and Palk Bay, Southeast coast of India. Indian Journal of Geo-Marine Sciences, 29(2):116-119
    Prakash Jebakumar J P, Nandhagopal G, Sundradarajan S, et al. 2015. Eminence of heavy metal accumulation in fishes and crustaceans from the Gulf of Khambhat, India. Current Science, 109(3):409-412
    Prymaczok N C, Pasqualino V M, Viau V E, et al. 2016. Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress. Journal of Comparative Physiology B, 186(2):181-191
    Rainbow P S. 1988. The significance of trace metal concentrations in decapods. Symposia of the Zoological Society of London, 59:291-313
    Rainbow P S. 1997. Ecophysiology of trace metal uptake in crustaceans. Estuarine, Coastal and Shelf Science, 44(2):169-176
    Reddy P S, Bhagyalakshmi A. 1994. Changes in oxidative metabolism in selected tissues of the crab (Scylla serrata) in response to cadmium toxicity. Ecotoxicology and Environmental Safety, 29(3):255-264
    Reddy P S, Devi M, Sarojini R, et al. 1994. Cadmium chloride induced hyperglycemia in the red swamp crayfish, Procambarus clarkii:Possible role of crustacean hyperglycemic hormone. Comparative Biochemistry and Physiology Part C:Pharmacology, Toxicology and Endocrinology, 107(1):57-61
    Reddy P S, Katyayani R V, Fingerman M. 1996. Cadmium and naphthalene-induced hyperglycemia in the fiddler crab, Uca pugilator:differential modes of action on the neuroendocrine system. Bulletin of Environmental Contamination and Toxicology, 56(3):425-431
    Reddy P S, Reddy P R, Sainath S B. 2011. Cadmium and mercury-induced hyperglycemia in the fresh water crab, Oziotelphusa senex senex:involvement of neuroendocrine system. Ecotoxicology and Environmental Safety, 74(3):279-283
    Reddy P S, Tuberty S R, Fingerman M. 1997. Effects of cadmium and mercury on ovarian maturation in the red swamp crayfish, Procambarus clarkii. Ecotoxicology and Environmental Safety, 37(1):62-65
    Santos E A, Keller R. 1993. Regulation of circulating levels of the crustacean hyperglycemic hormone:evidence for a dual feedback control system. Journal of Comparative Physiology B, 163(5):374-379
    Santos E A, Keller R, Rodriguez E, et al. 2001. Effects of serotonin and fluoxetine on blood glucose regulation in two decapod species. Brazilian Journal of Medical and Biological Research, 34(1):75-80
    Sedlmeier D. 1982. The mode of action of the crustacean neurosecretory hyperglycemic hormone (CHH):Ⅱ. Involvement of glycogen synthase. General and Comparative Endocrinology, 47(4):426-432
    Sedlmeier D. 1985. Mode of action of the crustacean hyperglycemic hormone. American Zoologist, 25(1):223-232
    Stentiford G D, Chang E S, Chang S A, et al. 2001. Carbohydrate dynamics and the crustacean Hyperglycemic hormone (CHH):effects of parasitic infection in Norway lobsters (Nephrops norvegicus). General and Comparative Endocrinology, 121(1):13-22
    Samuel N J, Thirunavukkarasu N, Soundarapandian P, et al. 2004. Fishery potential of commercially important portunid crabs along Parangipettai coast. In:Proceedings of Ocean Life Food and Medicine Expo, 165-173
    Smith H. 1982. Blue crabs in South Australia:their status, potential and biology. South Australian Fishing Industry Council, 6:6-9
    Tietz N W. 1976. Clinical Guide to Laboratory Tests. London:W B Saunders Company Ltd, 238
    Ui J. 1972. A few coastal pollution problems in Japan. In:Dyrssen D, Jagner D, eds. The Changing Chemistry of the Oceans. Stockholm:Almqvist and Wiksell, 364
    Vernberg W B, DeCoursey P J, Padgett W J. 1974. Synergistic effects of environmental variables on larvae of Uca pugilator. Marine Biology, 22(4):307-312
    Webster S G. 1996. Measurement of crustacean hyperglycaemic hormone levels in the edible crab Cancer pagurus during emersion stress. The Journal of Experimental Biology, 199(7):1579-1585
    Weis J S. 1978. Interactions of methylmercury, cadmium, and salinity on regeneration in the fiddler crabs Uca pugilator, U. pugnax and U. minax. Marine Biology, 49(2):119-124
    Weis J S. 1980. Effect of zinc on regeneration in the fiddler crab Uca pugilator and its interactions with methylmercury and cadmium. Marine Environmental Research, 3(4):249-255
    Potter I C, Chrystal P J, Loneragan N R. 1983. The biology of the blue manna crab Portunus pelagicus in an Australian estuary. Marine Biology, 78:75-85
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (713) PDF downloads(586) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return