Yaxi Chao, Shuyan Wang, Suqi Wu, Jiaqiang Wei, Hao Chen. Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5[J]. Acta Oceanologica Sinica, 2019, 38(6): 65-74. doi: 10.1007/s13131-018-1226-0
Citation: Yaxi Chao, Shuyan Wang, Suqi Wu, Jiaqiang Wei, Hao Chen. Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5[J]. Acta Oceanologica Sinica, 2019, 38(6): 65-74. doi: 10.1007/s13131-018-1226-0

Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5

doi: 10.1007/s13131-018-1226-0
Funds:  The Marine Public Welfare Project of SOA under contract No. 201505032; the Scientific and Technological Innovation Project financially of Qingdao National Laboratory for Marine Science and Technology under contract No. 2016ASKJ14.
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  • Corresponding author: E-mail: hchen@fio.org.cn
  • Received Date: 2018-02-02
  • Accepted Date: 2018-03-22
  • Available Online: 2020-04-21
  • Publish Date: 2019-06-01
  • Seven bacterial clones with alginate-utilizing activity were isolated from rotten kelp. By activity test, the Vibrio sp. QD-5 with the potential alginate-degrading capability was chosen to carry out the draft genome sequencing, and the result showed that the Vibrio sp. QD-5 containing an alginate lyase gene cluster. One of these genes, aly-IV, was cloned and characterized for the first time. After overexpression, Aly-IV, with a molecular mass of about 62 kDa and a theoretical isoelectric point (pI) of 5.12, was purified to a specific activity of 1 256.78 U/mg and showed highest activity at 35°C in the Tris-HCl buffer at pH of 8.9. Moreover, the enzyme activity was enhanced by the metal ions of Na+, K+ and Mg2+ under certain concentration. Aly-IV degraded favorably polyG blocks in an endo-type, yielding monomer and dimer as the main products. Due to its high substrate specificity, Aly-IV could be used as a potential tool for production of polyG oligosaccharides with low degree of polymerization (DP) and for determining the fine structure of alginate.
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