Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic <i>Vibrio harveyi</i>

QIN Yingxue; YAN Qingpi; SU Yongquan; LI Haiping; ZOU Wenzheng

doi:10.1007/s13131-013-0341-1

doi: 10.1007/s13131-013-0341-1

1.
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China;Fisheries College, Jimei University, Xiamen 361021, China
2.
College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China

基金项目: The National Natural Science Foundation of China under contract Nos 31272699 and 41176115; National Department Public Benefit Research Foundation of China under contract No. 200903029; the Natural Science Foundation of Fujian Province under contract No. 2011J06014; the National Hi-Tech Research and Development Programof China (863 Program) under contract No. 2007AA09Z115.

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- 收稿日期: 2012-04-09
- 修回日期: 2012-12-07

Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi

1.
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China;Fisheries College, Jimei University, Xiamen 361021, China
2.
College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China

摘要

摘要: Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (GenBank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (GenBank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth,motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energymetabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotacticmotility of V. harveyi.
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Abstract: Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (GenBank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (GenBank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth,motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energymetabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotacticmotility of V. harveyi.
- chemotactic motility /
- Vibrio harveyi /
- transposon /
- virulence

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参考文献(38)

Austin B, Zhang Xiaohua. 2006. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Lett Appl Microbiol, 43: 119-124

Balebona MC,MoriNigoMA, Faris A, et al. 1995. Influence of salinity and pH on the adhesion of pathogenic Vibrio strains to Sparus aurata skinmucus. Aquaculture, 132: 113-120

Banks R D, Blake C C, Evans P R, et al. 1979. Sequence, structure and activity of phosphoglycerate kinase: a possible hinge-bending enzyme. Nature, 279: 773-777

Boin M A, Austin M J, Hāse C C. 2004. Chemotaxis in Vibrio cholerae. FEMSMicro Lett, 239: 1-8

BordasMA, BalebonaMC, Rodriguez-Maroto JM, et al. 1998. Chemotaxis of pathogenic Vibrio strains towards mucus surfaces of gilt-head sea bream(Sparus aurata L.). Appl EnvironMicrobiol,64: 1573-1575

Butler S M, Camilli A. 2004. Both chemotaxis and net motility greatly influence the infectivity of Vibrio cholerae. PNAS, 101: 5018- 5023

Celani A, Vergassola M. 2010. Bacterial strategies for chemotaxis response. PANS, 107(4): 1391-1396

Deeraksa A, Moonmangmee S, Toyama H, et al. 2005. Characterization and spontaneous mutation of a novel gene, polE, involved in pellicle formation in Acetobacter tropicalis SKU1100. Microbiology, 151: 4111-4120

Haniford D B. 2006. Transpososome dynamics and regulation in Tn10 transposition. Crit Rev Biochem Mol Biol, 42: 407-424

HerreroM, Lorenzo V, Timmis K N. 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J Bacteriol, 172: 6557-6567

Josenhans C, Labigne A, Suerbaum S. 1995. Comparative ultrastructural and functional studies of Helicobacter pylory and Heli- cobacter mustelae flagellin mutants: both flagellin subunits, FlaA and FlaB, are necessary for full motility in Helicobacter species. J Bacteriol, 177: 3010-3020

Josenhans C, Suerbaum S. 2002. The role of motility as a virulence factor in bacteria. Int JMed Microbiol, 291: 605-614

Khanna M R, Bhavsar S P, Kapadnis B P. 2006. Effect of temperature on growth and chemotactic behaviour of Campylobacter jejuni. Lett ApplMicrobiol, 43: 84-90

Kirby J R. 2009. Chemotaxis-like regulatory systems: unique roles in diverse bacteria. Annual Review of Microbiology, 63: 45-59

Kleckner N. 1989. Transposon Tn10. In: Berg D E, Howe M M, eds. Mobile DNA. Washington D C: American Society for Microbiology Press

Klesius P H, Shoemaker C A, Evans J J. 2008. Flavobacterium columnare chemotaxis to channel catfish mucus. FEMS Microbiology Letters, 288(2): 216-220

Lacal J, Muñoz-Martínez F, Reyes-Darías J, et al. 2011. Bacterial chemotaxis towards aromatic hydrocarbons in Pseudomonas. EnvironmentalMicrobiology, 13(7): 1733-1744

Larsen M H, Larsen J L, Olsen J F. 2001. Chemotaxis of Vibrio anguil- larum to fish mucus: role of the origin of the fish mucus, the fish species and the serogroup of the pathogen. FEMS Microbiol Ecol, 38: 77-80

Lee S E, Kim S Y, Jeong B C, et al. 2006. A bacterial flagellin, Vibrio vulnificus FlaB, has a strongmucosal adjuvant activity to induce protective immunity. Infec Immun, 74: 694-702

Liu Yaoguang, Mitsukawa N, Oosumi T, et al. 1995. Efficient isolation andmapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J, 8: 457-463

Lux R, Moter A, Shi Wenyuan. 2000. Chemotaxis in pathogenic spirochetes: directed movement toward targeting tissues. J Mol Microbiol Biotechnol, 2: 355-364

Lux R, Shi Wenyuan. 2004. Chemotaxis-guided movements in bacteria. Crit Rev Oral Biol Med, 15: 207-220

Macnab R M. 1996. Flagella and motility. In: Neidhardt F C, ed. Escherichia coli and Salmonella: Cellular and Molecular Biology. Washington D C: ASMPress

Merrell D S, Butler S M, Qadri F, et al. 2002. Host-induced epidemic spread of the cholera bacterium. Nature, 417: 642-645

McCarter L L. 2001. Polar flagellarmotility of the Vibrionaceae. MicrobiolMol Biol Rev, 65: 445-462

O'Toole R, Miotom D L, Wolf-Watz H. 1996. Chemotactic motility is required for invasion of the host by the fish pathogen Vibrio anguillarum. MolMicrobiol, 19: 625-637

Porter S L, Wadhams G H, Armitage J P. 2011. Signal processing in complex chemotaxis pathways. Nature Reviews Microbiology, 9: 153-165

Qin Yingxue, Wang Jun, Su Yongquan, et al. 2006. Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara. Acta Oceanol Sin, 25: 147-153

Rock J L, Nelson D R. 2006. Identification and characterization of a hemolysin gene cluster in Vibrio anguillarum. Infec Immun, 74: 2777-2786

Salvetti S, Celandroni F, CeragioliM, et al. 2009. Identification of nonflagellar genes involved in swarm cell differentiation using a Bacillus thuringiensis mini-Tn10 mutant library. Microbiology, 155: 912-921

Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular Cloning: A LaboratoryManual. NewYork: Cold SpringHarbor Laboratory Press

Sawabe T, Inoue S, Fukui Y, et al. 2007. Mass mortality of Japanese abalone Haliotis discus hannai caused by Vibrio harveyi infection. Microbes Environ, 22: 300-308

Spohn G, Scarlato V. 2001. Motility, Chemotaxis, and Flagella. In: Mobley H L T, Mendz G L, Haze U S L, eds. Helicobacter pylory: Physiology and Genetics. Washington D C: ASMPress

Valenzuela M, Cerda O, Toledo H. 2003. Overview on chemotaxis and acid resistance in Helicobacter pylori. Biol Res, 36: 429-436

VineNG, LeukesWD, Kaiser H. 2004. In vitro growth characteristics of five candidates aquaculture probiotics and two fish pathogens grown in fish intestinal mucus. FEMS Microbiol Lett, 231: 145- 152

Vladimirov N, Sourjik V. 2009. Chemotaxis: how bacteria usememory. Biological Chemistry, 390(11): 1097-1104

Xie L, Altindal T, Chattopadhyay S, et al. 2011. Bacterial flagellum as a propeller and as a rudder for efficient chemotaxis. PNAS, 108(6): 2246-2251

Yan Qingpi, Zhao Minhui, Wang Xiaolu, et al. 2010. Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara. Chinese Journal of Oceanology and Limnology, 28(2): 260-266

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doi: 10.1007/s13131-013-0341-1

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Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi

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