Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

Wu Yuehong Cao Yi Wang Chunsheng Wu Min Aharon Oren Xu Xuewei

吴月红, 曹佚, 王春生, 吴敏, AharonOren, 许学伟. 西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性[J]. 海洋学报英文版, 2014, 33(10): 94-104. doi: 10.1007/s13131-014-0544-0
引用本文: 吴月红, 曹佚, 王春生, 吴敏, AharonOren, 许学伟. 西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性[J]. 海洋学报英文版, 2014, 33(10): 94-104. doi: 10.1007/s13131-014-0544-0
Wu Yuehong, Cao Yi, Wang Chunsheng, Wu Min, Aharon Oren, Xu Xuewei. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge[J]. Acta Oceanologica Sinica, 2014, 33(10): 94-104. doi: 10.1007/s13131-014-0544-0
Citation: Wu Yuehong, Cao Yi, Wang Chunsheng, Wu Min, Aharon Oren, Xu Xuewei. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge[J]. Acta Oceanologica Sinica, 2014, 33(10): 94-104. doi: 10.1007/s13131-014-0544-0

西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性

doi: 10.1007/s13131-014-0544-0
基金项目: The China Ocean Mineral Resources R & D Association (COMRA) Special Foundation under contract No. DY125-15-R-03; the National Natural Science Foundation of China under contract Nos 41276173 and 41206104; the Scientific Research Fund of the Second Institute of Oceanography, State Oceanic Administration under contract No. JT1011.

Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

  • 摘要: 沉积物样品来自西南印度洋深海热液硫化物区,水深为2951米。通过扩增其16S rRNA 和nifH基因研究微生物群落结构。古菌克隆主要为MBGE(Marine Benthic Group E)和MGI (Marine GroupⅠ)类群,分别属于广古菌门和奇古菌门。超过一半的细菌克隆属于变形杆菌(Proteobacteria),且大部分为γ-变形菌纲。未检测到ε-变形杆菌纲的克隆。此外还检测到放线菌门、拟杆菌门、浮霉菌门、酸杆菌门、消化螺旋菌门、绿弯菌门、绿菌门、衣原体门、疣微菌门和几个候补类群(OD1、 OP11、WS3、TM6),表明这些类群存在于热液硫化物环境。固氮基因(nifH)研究揭示了热液硫化物环境存在生物氮固定过程。系统发育分析表明该环境只存在类群I和类群III固氮基因。这和16S rRNA系统发育分析结果一致,表明细菌在热液硫化物环境氮固定过程中可能发挥了主要作用。
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  • 收稿日期:  2013-03-25
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