Microbial community structure of Arctic seawater as revealed by pyrosequencing

LI Yang; WANG Zhen; LIN Xuezheng

doi:10.1007/s13131-015-0742-4

通过焦磷酸测序揭示北极海水的微生物群落结构

doi: 10.1007/s13131-015-0742-4

国家海洋局第一海洋研究所, 山东青岛 266061;国家海洋局海洋生物活性物质重点实验室, 山东青岛 266061

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- 收稿日期: 2015-03-09
- 修回日期: 2015-08-07

Microbial community structure of Arctic seawater as revealed by pyrosequencing

The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Key Laboratory of Marine Bioactive Substances, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 这项研究的目的在于确定北极海水浮冰区内(ICE-1)和浮冰区外(FUBIAO)的微生物群落结构。将近10 L海水用孔径为0.2μm的Whatman滤膜过滤后提取环境基因组DNA并用焦磷酸测序对其微生物群落进行详细研究。微生物群落结构分析表明两个样本在细菌、古菌和真核生物中存在高度多样性。两样本中变形菌门和拟杆菌门是主要的浮游细菌群落,相对丰度分别为51.29% 和 35.39% (FUBIAO), 72.95%和 23.21%(ICE-1)。古菌门中最丰富的是广古菌门,相对丰度分别为100% (FUBIAO)和60%(ICE-1)。真核生物中,FUBIAO中含量最丰富的为没有明确分类的真核生物,节肢动物以及没有明确分类的后生动物,三者占据了85.29%;ICE-1中含量最丰富的为没有明确分类的真核生物及没有明确分类的后生动物,达到读取总量的90.69%。α-变形菌、黄杆菌及γ-变形菌是两个样本中纲水平上含量最丰富的三种细菌,同时,在97%相似度水平上,两样本中细菌、古菌以及真核生物中前十中最丰富的OTUs也存在很大不同。
- 北极海水 /
- 焦磷酸测序 /
- 微生物群落
Abstract: This study aimed to determine the microbial community structure of seawater in (ICE-1) and out (FUBIAO) of the pack ice zone in the Arctic region. Approximate 10 L seawater was filtrated by 0.2 μm Whatman nuclepore filters and the environmental genomic DNA was extracted. We conducted a detailed census of microbial communities by pyrosequencing. Analysis of the microbial community structures indicated that these two samples had high bacterial, archaeal and eukaryotic diversity. Proteobacteria and Bacteroidetes were the two dominant members of the bacterioplankton community in both samples, and their relative abundance were 51.29% and 35.39%, 72.95% and 23.21%, respectively. Euryarchaeota was the most abundant archaeal phylum, and the relative abundance was nearly up to 100% in FUBIAO and 60% in ICE-1. As for the eukaryotes, no_rank_Eukaryota, Arthropoda and no_rank_Metazoa were the most abundant groups in Sample FUBIAO, accounting for 85.29% of the total reads. The relative abundance of the most abundant phylum in Sample ICE-1, no_rank_Eukaryota and no_rank_Metazoa, was up to 90.69% of the total reads. Alphaproteobacteria, Flavobacteria and Gammaproteobacteria were the top three abundant classes in the two samples at the bacterial class level. There were also differences in the top ten abundant bacterial, archaeal and eukaryotic OTUs at the level of 97% similarity between the two samples.
- Arctic seawater /
- pyrosequencing /
- microbial community

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通过焦磷酸测序揭示北极海水的微生物群落结构

doi: 10.1007/s13131-015-0742-4

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Microbial community structure of Arctic seawater as revealed by pyrosequencing

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