Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga <i>Isochrysis zhanjiangenisis</i>

YAO Jingyuan; LIU Yu; LI Ying; WANG Haixia

doi:10.1007/s13131-016-0837-6

预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响

doi: 10.1007/s13131-016-0837-6

1.
环境科学与工程学院, 大连海事大学, 大连 116026, 中国
2.
环境信息研究所, 大连海事大学, 大连 116026, 中国

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- 收稿日期: 2015-06-23
- 修回日期: 2015-11-24

Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga Isochrysis zhanjiangenisis

1.
College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China
2.
Environmental Information Institute, Dalian Maritime University, Dalian 116026, China

摘要

摘要: 本研究量化了不同预处理方法对海洋微藻(Isochrysis zhanjiangenisis)脂肪酸稳定碳同位素组成的影响。为确定样品预处理对微藻脂肪酸δ¹³C及脂肪酸含量的影响,采用八种预处理方法,包括:(a) 干燥样品,HCl-CH₃OH直接甲酯化;(b) 干燥样品,H₂SO₄-CH₃OH直接甲酯化;(c) 干燥样品,超声萃取后HCl-CH₃OH甲酯化;(d) 干燥样品,超声萃取后H₂SO₄-CH₃OH 甲酯化;(e) 新鲜样品,HCl-CH₃OH直接甲酯化;(f) 新鲜样品,H₂SO₄-CH₃OH直接甲酯化;(g) 新鲜样品,超声萃取后HCl-CH₃OH甲酯化,以及 (h) 新鲜样品,超声萃取后H₂SO₄-CH₃OH 甲酯化。结果表明:实验组 a-e, g 和 h中脂肪酸甲酯δ¹³C在0.3‰内波动,且实验组 f中脂肪酸甲酯δ¹³C低于其他组约0.7‰。因此,不同的海洋微藻预处理方法可能导致不同脂肪酸稳定碳同位素比,应给予适当修正。
- 碳稳定同位素分析 /
- 脂肪酸 /
- 海洋微藻 /
- 预处理
Abstract: This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae (Isochrysis zhanjiangenisis). To identify the effects of sample preparation on the δ¹³C value and the fatty acid composition, we examined eight types of pretreatment methods including: (a) drying the sample followed by direct methyl esterification using HCl-CH₃OH; (b) drying the sample followed by direct methyl esterification using H₂SO₄-CH₃OH; (c) drying the sample by ultrasonic extraction and methyl-esterification using HCl-CH₃OH; (d) drying the sample by ultrasonic extraction and methyl-esterification using H₂SO₄-CH₃OH; (e) fresh sample followed by direct methyl-esterification using HCl-CH₃OH; (f) fresh sample followed by direct methyl-esterification using H₂SO₄-CH₃OH; (g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH₃OH, and (h) fresh sample with ultrasonic extraction followed by methyl-esterification using H₂SO₄-CH₃OH. The results show that the δ¹³C values from Groups a-e, g and h fluctuated within 0.3‰, and the δ¹³C values of Group f were approximately 0.7‰ lower than the other seven groups. Therefore, the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios, and if necessary a correction should be applied.
- carbon stable isotope analysis /
- fatty acid /
- marine microalgae /
- pretreatment

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参考文献(28)

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预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响

doi: 10.1007/s13131-016-0837-6

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Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga Isochrysis zhanjiangenisis

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