Artificial seed germination and seedling production of Zostera marina L. by salinity manipulation
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摘要: 近年来大叶藻海草场已发生急剧衰退,亟需得到修复。我们发现通过人为调控大叶藻种子及萌发后种子培养过程的盐度,能有效促进种子萌发及建成幼苗,提高利用种子繁殖修复海草场的效率。本文结果显示降低盐度可显著提高大叶藻种子萌发率(GR)、缩短平均萌发时间(MTG),盐度0具最高促萌效果。经盐度0培养所萌发种子能于盐度5-30中形成幼苗并继续发育。萌发后于盐度5-15中培养40 d,经淡水培养1-20 d而萌发种子的生长发育未呈现显著差异。转移盐度0或5中萌发种子至天然海水(盐度30)继续培养的过程中,渐升盐度梯度的海水驯化处理能显著提高种子形成幼苗的比例,盐度梯度升高幅度愈小愈利于种子发育和存活。通过先盐度0的淡水处理大叶藻种子20 d、后盐度渐升至30的海水培育萌发种子40 d这一技术,终有33.0%种子在2个月内于盐度30天然海水中建成了幼苗,显示出人为调控盐度策略提高种子建苗率的有效性、实用性,为大叶藻海草场修复提供了技术参考。Abstract: Vast declines in Zostera marina seagrass beds demand effective methods of rehabilitation. In this study, we developed a practical method by reducing salinity to induce seed germination followed with recovering salinity to facilitate seedling production of Z. marina. The results showed that Z. marina seeds collected from natural seawater (salinity 30) were induced to germinate at reduced salinities. Percent germination (GR) was higher and mean-time-to-germinate (MTG) was shorter at lower salinities. The highest GR and shortest MTG occurred at salinity 0 (deionized freshwater). After germination in freshwater, seeds could develop into seedlings at salinities 5-30 and continue the growth. Viability or development of germinated seeds was not significantly different during the 40 d of post-germination incubation at salinities 5-15 after 1-20 d of germination in freshwater. However, during the process of translating germinated seeds from salinity 0 and 5 to salinity 30, reducing the gradients of post-germination acclimation facilitated more seeds forming seedlings in less time. On average, after 60 d of static incubation, including 20 d in freshwater for germination followed with immediate shift to salinity 5 and increasing to salinity 30 at increment of 5 every two days until cultivation at constant salinity 30, 33% of Z. marina seeds produced healthy seedlings. The results indicate that the salinity-manipulation based method of artificial germination and seedling production is practical and effective in supporting rehabilitation of Z. marina bed.
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Key words:
- Zostera marina L. /
- rehabilitation /
- seed /
- germination /
- seedling /
- salinity
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