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Flow cytometric analysis of DNA content for four commercially important crabs in China

LIU Lei,  CUI Zhaoxia,  SONG Chengwen,  LIU Yuan,  HUI Min,  WANG Chunlin

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文章导航 > Acta Oceanologica Sinica  > 2016 >  35(6): 7-11
刘磊, 崔朝霞, 宋呈文, 刘媛, 惠敏, 王春琳. 应用流式细胞术测定中国四种经济蟹类的基因组大小[J]. 海洋学报英文版, 2016, 35(6): 7-11. doi: 10.1007/s13131-016-0876-z
引用本文: 刘磊, 崔朝霞, 宋呈文, 刘媛, 惠敏, 王春琳. 应用流式细胞术测定中国四种经济蟹类的基因组大小[J]. 海洋学报英文版, 2016, 35(6): 7-11. doi: 10.1007/s13131-016-0876-z
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LIU Lei, CUI Zhaoxia, SONG Chengwen, LIU Yuan, HUI Min, WANG Chunlin. Flow cytometric analysis of DNA content for four commercially important crabs in China[J]. Acta Oceanologica Sinica, 2016, 35(6): 7-11. doi: 10.1007/s13131-016-0876-z
Citation: LIU Lei, CUI Zhaoxia, SONG Chengwen, LIU Yuan, HUI Min, WANG Chunlin. Flow cytometric analysis of DNA content for four commercially important crabs in China[J]. Acta Oceanologica Sinica, 2016, 35(6): 7-11. doi: 10.1007/s13131-016-0876-z
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应用流式细胞术测定中国四种经济蟹类的基因组大小

doi: 10.1007/s13131-016-0876-z
  • 1.

    宁波大学海洋学院, 宁波 315211

  • 2.

    中国科学院海洋研究所, 青岛 266071

Flow cytometric analysis of DNA content for four commercially important crabs in China

  • 1.

    School of Marine Sciences, Ningbo University, Ningbo 315211, China

  • 2.

    Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

    摘要
  • 摘要: 本研究以鸡(Gallus domesticus)的基因组大小(2.5 pg/2C)为内标,采用流式细胞术首次测定了我国沿海常见四种经济蟹类,三疣梭子蟹(Portunus trituberculatus),日本蟳(Charybdis japonica),拟穴青蟹(Scylla paramamosain)和中华绒螯蟹(Eriocheir sinensis)的基因组大小。结果表明,三疣梭子蟹,日本蟳,拟穴青蟹和中华绒螯蟹的基因组大小分别为2.31±0.01 pg, 2.33±0.03 pg, 1.64±0.02 pg, and 2.29±0.03 pg。这四种蟹的基因组含量小于甲壳动物基因组含量平均值(4.99±0.48 pg),且其中的三疣梭子蟹,日本蟳和中华绒螯蟹基因组大小相近,推测可能与四种蟹的地理分布相关。本研究为这四种蟹的生物多样性、物种保护和系统发育研究及杂交育种和基因组测序计划的实施提供数据支持。此外,本文建立了应用血细胞进行蟹类基因组大小测定的操作方法,为其它甲壳动物的基因组测定提供参考。
    Abstract: The genome size (C-value) of an organism is referring to the DNA content of its non-replicated haploid chromosome complement, generally deduced from measuring somatic diploid nuclei. We presented genome size (C-value) data obtained by flow cytometry for four commercially important crabs (Portunus trituberculatus, Charybdis japonica, Scylla paramamosain, and Eriocheir sinensis) common in the coast of China. Gallus domesticus (2C=2.5 pg) was used as the internal standard. The results showed that the C-value for P. trituberculatus, C. japonica, S. paramamosain, and E. sinensis were (2.31±0.01) pg, (2.33±0.03) pg, (1.64±0.02) pg, and (2.29±0.03) pg, respectively. The C-value of P. trituberculatus, C. japonica and S. paramamosain were reported for the first time. The data represented by the four species indicated that they had lower DNA contents than average DNA values in crustaceans ((4.99±0.48) pg), and three of the four values were very similar if not identical. The results provide useful data for future studies in the fields of biodiversity, species conservation, and phylogeny of these commercial crabs. They will also be helpful in instructing the hybridization breeding program and estimating the cost of the whole genome sequencing project.
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出版历程
  • 收稿日期:  2015-04-02
  • 修回日期:  2015-12-16

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