Transcriptome analysis of the Antarctic psychrotrophic bacterium <i>Psychrobacter</i> sp. G in response to temperature stress

WANG Zhen; LI Yang; LIN Xuezheng

doi:10.1007/s13131-016-0956-0

南极适冷菌菌株G在温度胁迫下的转录组分析

doi: 10.1007/s13131-016-0956-0

国家海洋局第一海洋研究所, 海洋生物活性物质重点实验室, 山东青岛, 266061

基金项目: The National Natural Science Foundation of China under contract No. 41176174; the Chinese Polar Environment Comprehensive Investigation and Assessment Program under contract No. CHINARE 2014-03-05; the Public Science and Technology Funds for Ocean Projects under contract No. 201205020-5.

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- 收稿日期: 2015-08-31
- 修回日期: 2016-05-15

Transcriptome analysis of the Antarctic psychrotrophic bacterium Psychrobacter sp. G in response to temperature stress

The First Institute of Oceanography, Key Laboratory of Marine Bioactive Substances, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 南极适冷菌菌株G中参与温度适应的关键功能基因是通过转录组测序确定的。我们分析了在冷激条件（0℃）和热激条件（30℃）下菌株G的转录情况，并把最适生长温度20℃作为对照组。冷激/热激后，转录组情况发生了很大的变化，与对照组相比，分别有11和12个基因表达显著上调，同时有47和42个基因显著下调。热激条件下编码酶和转录调控因子的基因，包括PfpI和TetR家族转录调节因子以及冷激条件下编码DEAD/DEAH解旋酶和IclR家族转录调节因子的基因，其表达量均显著上升。另外一些基因的表达，主要是与Ton-B依赖性受体和铁载体受体有关的基因，其表达量无论在冷激还是热激条件下，均显著下降。许多与脂肪酸代谢和ABC运输有关的基因在不同温度条件下的表达情况有所不同。对转录组和温度胁迫相关基因的调查结果有助于我们了解南极细菌的抗逆力机制。
- 适冷菌 /
- 温度胁迫 /
- 转录组 /
- 南极
Abstract: The key functional genes involved in temperature adaption of the Antarctic psychrotrophic bacterium Psychrobacter sp. G. were identified by transcriptomic sequencing. We analyzed the global transcriptional profile of Psychrobacter sp. G under cold stress (0℃) and heat stress (30℃), with the optimal growth temperature 20℃ as the control. There were large alterations of the transcriptome profile, including significant upregulation of 11 and 12 transcripts as well as significant downregulation of 47 and 42 transcripts in the cold and heat stress groups, respectively, compared to the control. The expression of various genes encoding enzymes and transcriptional regulators, including PfpI and TetR family transcriptional regulators under heat stress, as well as the expression of DEAD/DEAH box helicase and the IclR family of transcriptional regulators under cold stress, were upregulated significantly. The expression of several genes, most affiliated with TonB-dependent receptor and siderophore receptor, was downregulated significantly under both heat and cold stress. Many of the genes associated with the metabolism of fatty acid and ABC transporters were regulated differentially under different temperature stress. The results of this survey of transcriptome and temperature stress-relevant genes contribute to our understanding of the stress-resistant mechanism in Antarctic bacteria.
- Psychrobacter /
- temperature stress /
- transcriptome /
- Antarctic

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参考文献(45)

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南极适冷菌菌株G在温度胁迫下的转录组分析

doi: 10.1007/s13131-016-0956-0

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Transcriptome analysis of the Antarctic psychrotrophic bacterium Psychrobacter sp. G in response to temperature stress

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