Autotrophic potential in mesophilic heterotrophic bacterial isolates from Sino-Pacific marine sediments

CAO Wenrui; DAS Anindita; SAREN Gaowa; JIANG Mingyu; ZHANG Hongjie; YU Xinke

doi:10.1007/s13131-016-0962-2

中国周边太平洋海底沉积物中分离的嗜温异养菌株的自养潜能

doi: 10.1007/s13131-016-0962-2

1.
中国科学院海洋研究所, 海洋地质与环境重点实验室, 青岛 266071, 中国
2.
中国科学院海洋研究所, 海洋地质与环境重点实验室, 青岛 266071, 中国;印度国家海洋研究所, 科学和工业研究委员会, 果阿 403004, 印度;马哈拉施特拉科学种植协会, Agharkar研究所, 马哈拉施特拉 411004, 印度
3.
中国科学院生物物理研究所, 北京 100101, 中国

基金项目: The National Natural Science Foundation of China under contract Nos 41406062 and 41250110530; the Chinese Academy of Science Fellowship for Young Foreign Scientists under contract No. 2012Y1ZA0005.

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- 收稿日期: 2016-06-24
- 修回日期: 2016-10-26

Autotrophic potential in mesophilic heterotrophic bacterial isolates from Sino-Pacific marine sediments

1.
Key laboratory of Marine Geology and Environment(MAGE), Institute of Oceanology, Chinese Academy of Sciences(IO-CAS), Qingdao 266071, China
2.
Key laboratory of Marine Geology and Environment(MAGE), Institute of Oceanology, Chinese Academy of Sciences(IO-CAS), Qingdao 266071, China;Council of Scientific and Industrial Research(CSIR), National Institute of Oceanography, Dona Paula, Panaji 403004, India;Maharashtra Association for the Cultivation of Science(MACS), Agharkar Research Institute, Pune 411004, India
3.
Institute of Biophysics, Chinese Academy of Sciences(IBP-CAS), Beijing 100101, China

摘要

摘要: 微生物固碳作用海洋生态系统中非常重要的生物过程，尤其是在阳光无法到达的深水及深海沉积物中。众所周知，自养微生物以可以固定二氧化碳而闻名，但是异养生物是否也能固碳是个耐人寻味的问题。10个分离自中国周边太平洋海底沉积物中的微生物菌株，在添加不同的电子供体后，被用来测定其自养潜能。在不添加其他任何电子供体时，10株细菌中的9株细菌展现出其具有固定碳的能力，但是其效率却相对较低，为10^-8 to 10^-4 fmol C cell^-1 hr^-1。这些菌株在终浓度为1mM的Mn²⁺或NH₄⁺的体系中，其碳固定效率有所增强；而在终浓度为1mM的Fe²⁺或S^2-的体系中，其碳固定效率有所降低。此外，有些菌株对甲醛的耐受暗示着存在回补化学反应用于形成一碳代谢途径中间产物，这展现了这些菌株的代谢过程的灵活应变性。当电子供体发生变化时，这些菌株会在还原铁/硫的循环和氧化锰/铵的循环中寻找平衡。这些特性使得这些微生物成功地在高动态的中国周边太平洋沉积物中生存下来。
- 自养菌 /
- 细菌 /
- 嗜温菌 /
- 异养生物 /
- 中国太平洋
Abstract: Microbial carbon fixation is a paramount process in the ocean especially below the photic zone both in water and sedimentary ecosystems. Autotrophic microbes that fix carbon dioxide are renowned. However, the question whether heterotrophs can also fix carbon is intriguing. Ten heterotrophically grown, identified bacterial isolates from the Sino-Pacific marine sediments were tested for autotrophic uptake potential with and without addition of electron donors. Nine of the ten isolates showed carbon uptake capacity without addition of any substrate at very low rates in the order of 10^-8 to 10^-4 fmol/(cell·h). The addition of manganese and ammonium at 1 mmol/L final concentration enhanced the uptake potential. Addition of 1 mmol/L final concentrations of reduced iron (10^-6 to 10^-5 fmol/(cell·h) and sulfide (10^-5 fmol/(cell·h) decreased the uptake potential significantly at p<0.1. Bacterial tolerance to formaldehyde suggested propensities of anaplerotic chemical reactions that form metabolic intermediates of C-1 metabolism pathways. The isolates displayed high metabolic flexibility. With the changes in electron donors, the isolates metabolically toggled between relatively anoxic reductive iron/sulfur cycles and the oxidative cycles of manganese/ammonium and vice-versa. This property makes these microbes successful survivors in the highly dynamic Sino-Pacific sediments.
- autotrophy /
- bacteria /
- mesophiles /
- heterotrophs /
- Sino-Pacific

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中国周边太平洋海底沉积物中分离的嗜温异养菌株的自养潜能

doi: 10.1007/s13131-016-0962-2

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Autotrophic potential in mesophilic heterotrophic bacterial isolates from Sino-Pacific marine sediments

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