Bacterial and archaeal community structure of pan-Arctic Ocean sediments revealed by pyrosequencing

LIN Xuezheng; ZHANG Liang; LIU Yanguang; LI Yang

doi:10.1007/s13131-017-1030-2

北极海洋沉积物细菌和古菌群落结构分析

doi: 10.1007/s13131-017-1030-2

国家海洋局第一海洋研究所, 青岛 266061

基金项目: The Chinese Polar Environment Comprehensive Investigation and Assessment Program under contract No. CHINARE2014-03-05; the National Natural Science Foundation of China under contract No. 41176174; the Innovation and Development Regional Demonstration Program of Marine Economy under contract No. 12PYY001SF08-HYYS-1.

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- 收稿日期: 2016-06-17

Bacterial and archaeal community structure of pan-Arctic Ocean sediments revealed by pyrosequencing

The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 利用焦磷酸测序技术对北极海洋沉积物的细菌和古菌群落结构特征进行了研究。从3个沉积物样品总计获得了15002个细菌和4362个古菌操作分类单元（operational taxonomic units，OTUs）。群落结构分析表明，3个沉积物样品均具有较高的细菌和古菌多样性。样品CC1和R05中变形菌门（Proteobacteria）的含量最为丰富，而样品BL03中厚壁菌门（Firmicutes）的含量最为丰富。奇古菌门（Thaumarchaeota）在样品CC1和R05中最为丰富，而样品BL03中奇古菌门和广古菌门（Euryarchaeota）的相对含量基本一样；这两个古菌门的相对含量几乎占古菌OTUs的100%。在变形菌门纲水平上，δ-和γ-变形菌纲的相对含量最为丰富，其相对含量可占样品CC1和R05细菌OTUs的60%。在97%相似性水平上，3个样品中相对含量最为丰富的10个细菌和古菌OTUs也存在着明显差异，只检测到12个细菌共有OTUs （core OTUs）。总之，本研究表明，北极海洋沉积物存在着独特的微生物群落和许多独特的OTUs。
- 北极沉积物 /
- 微生物群落 /
- 多样性 /
- 焦磷酸测序
Abstract: This study was to investigate bacterial and archaeal community structure of pan-Arctic Ocean sediments by pyrosequencing. In total, investigation of three marine sediments revealed 15 002 bacterial and 4 362 archaeal operational taxonomic units (OTUs) at the 97% similarity level. Analysis of community structure indicated that these three samples had high bacterial and archaeal diversity. The most relatively abundant bacterial group in Samples CC1 and R05 was Proteobacteria, while Firmicutes was dominant in Sample BL03. Thaumarchaeota was the most relatively abundant archaeal phylum in Samples CC1 and R05, and the relative abundance of Thaumarchaeota was almost as high as that of Euryarchaeota in Sample BL03. These two phyla accounted for nearly 100% of the archaeal OTUs. δ-Proteobacteria and γ-Proteobacteria were the two most relatively abundant classes at Proteobacterial class level, and their relative abundance was more than 60% in Samples CC1 and R05. There were also differences in the top 10 relatively abundant bacterial and archaeal OTUs among the three samples at the 97% similarity, and only 12 core bacterial OTUs were detected. Overall, this study indicated that there were distinct microbial communities and many unique OTUs in these three samples.
- Arctic sediment /
- microbial community /
- diversity /
- pyrosequencing

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参考文献(39)

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北极海洋沉积物细菌和古菌群落结构分析

doi: 10.1007/s13131-017-1030-2

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Bacterial and archaeal community structure of pan-Arctic Ocean sediments revealed by pyrosequencing

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