A novel beta-galactose-specific lectin of the tubeworm, Ridgeia piscesae, from the hydrothermal vent

RUAN Lingwei XU Haipeng LIN Wenyang SHI Hong CUI Zhizhong XU Xun

阮灵伟, 徐海鹏, 林文洋, 施泓, 崔治中, 徐洵. 深海热液区管状蠕虫Ridgeia piscesae β型半乳糖凝集素的克隆与功能研究[J]. 海洋学报英文版, 2017, 36(6): 61-67. doi: 10.1007/s13131-017-1052-9
引用本文: 阮灵伟, 徐海鹏, 林文洋, 施泓, 崔治中, 徐洵. 深海热液区管状蠕虫Ridgeia piscesae β型半乳糖凝集素的克隆与功能研究[J]. 海洋学报英文版, 2017, 36(6): 61-67. doi: 10.1007/s13131-017-1052-9
RUAN Lingwei, XU Haipeng, LIN Wenyang, SHI Hong, CUI Zhizhong, XU Xun. A novel beta-galactose-specific lectin of the tubeworm, Ridgeia piscesae, from the hydrothermal vent[J]. Acta Oceanologica Sinica, 2017, 36(6): 61-67. doi: 10.1007/s13131-017-1052-9
Citation: RUAN Lingwei, XU Haipeng, LIN Wenyang, SHI Hong, CUI Zhizhong, XU Xun. A novel beta-galactose-specific lectin of the tubeworm, Ridgeia piscesae, from the hydrothermal vent[J]. Acta Oceanologica Sinica, 2017, 36(6): 61-67. doi: 10.1007/s13131-017-1052-9

深海热液区管状蠕虫Ridgeia piscesae β型半乳糖凝集素的克隆与功能研究

doi: 10.1007/s13131-017-1052-9
基金项目: The Major State Basic Research Development Program of China (973 Program) under contract No. 2015CB755906; China Ocean Mineral Resources R&D Association under contract No. DYXM-115-02-2-16.

A novel beta-galactose-specific lectin of the tubeworm, Ridgeia piscesae, from the hydrothermal vent

  • 摘要: 凝集素是一类能够特异性地识别糖苷结构并与之结合的蛋白质或糖蛋白,其不仅在细胞识别与调控中发挥着重要的生理功能,而且在医药等多个领域具有广泛的应用价值。管状蠕虫是深海热液区的典型生物,其独特的生理特征使其能够应对深海热液区的极端环境。本研究对管状蠕虫Ridgeia piscesae的半乳糖凝集素RPGAL进行了克隆与功能研究。RPGAL编码基因长1092 bp,与已知的半乳糖凝集素的同源性较低,具有串联重复型半乳糖凝集素的典型特征,含有两个同源的糖识别结构域。通过利用大肠杆菌原核表达系统,RPGAL成功地进行了重组表达与纯化。生物活性分析表明RPGAL对所测试的脊椎动物血细胞均具有凝集效应,并且为金属非依赖型,其在温度10-50℃,pH5-10范围内均比较稳定,而其对血细胞的凝集活性可以被D-乳糖和脂多糖强烈抑制。此外,RPGAL对所测试的微生物没有凝集效应,但具有抗肿瘤活性,可促进Hela和HT1080的细胞凋亡。本研究表明RPGAL是一种新的半乳糖凝集素,其在抗肿瘤方面具有潜在的应用价值
  • Barondes S H, Castronovo V, Cooper D N W, et al. 1994a. Galectins:a family of animal β-galactoside-binding lectins. Cell, 76(4):597-598
    Barondes S H, Cooper D N, Gitt M A, et al. 1994b. Galectins:structure and function of a large family of animal lectins. J Biol Chem, 269(33):20807-20810
    Bernerd F, Sarasin A, Magnaldo T. 1999. Galectin-7 over expression is associated with the apoptotic process in UVB-induced sunburn keratinocytes. Proc Natl Acad Sci U S A, 96(20):11329-11334
    Boronkai A, Bellyei S, Szigeti A, et al. 2009. Potentiation of paclitaxel-induced apoptosis by galectin-13 overexpression via activation of Ask-1-p38-MAP kinase and JNK/SAPK pathways and suppression of Akt and ERK1/2 activation in U-937 human macrophage cells. Eur J Cell Biol, 88(12):753-763
    Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72(1-2):248-254
    Cominetti M R, Marques M R F, Lorenzini D M, et al. 2002. Characterization and partial purification of a lectin from the hemolymph of the white shrimp Litopenaeus schmitti. Dev Comp Immunol, 26(8):715-721
    Cooper D N W. 2002. Galectinomics:finding themes in complexity. Biochim Biophys Acta, 1572(2-3):209-231
    Cooper D N W, Barondes S H. 1999. God must love galectins; he made so many of them. Glycobiology, 9(10):979-984
    Dam T K, Sarkar M, Ghosal J, et al. 1992. A novel galactosyl-binding lectin from the plasma of the blood clam, Anadara granosa (L) and a study of its combining site. Mol Cell Biochem, 117(1):1-9
    Dhuna V, Kamboj S S, Kaur A, et al. 2007. Characterization of a lectin from Gonatanthus pumilus D. Don having anti-proliferative effect against human cancer cell lines. Protein Pept Lett, 14:71-78
    Fajka-Boja R, Szemes M, Ion G, et al. 2002. Receptor tyrosine phosphatase, CD45 binds galectin-1 but does not mediate its apoptotic signal in T cell lines. Immunol Lett, 82(1-2):149-154
    Fukumori T, Kanayama H O, Raz A. 2007. The role of galectin-3 in cancer drug resistance. Drug Resist Updat, 10(3):101-108
    Gamulin V, Rinkevich B, Schäcke H, et al. 1994. Cell adhesion receptors and nuclear receptors are highly conserved from the lowest Metazoa (marine sponges) to vertebrates. Biol Chem Hoppe Seyler, 375(9):583-588
    Gaphurov J M, Bulgakov A A, Galkin V V, et al. 1999. Some properties of alkaline DNases of tentacles of actinia Radianthus macrodactylus and their hemolytic activity. Toxicon, 37(11):1591-1604
    Giga Y, Ikai A, Takahashi K. 1987. The complete amino acid sequence of echinoidin, a lectin from the coelomic fluid of the sea urchin Anthocidaris crassispina, homologies with mammalian and insect lectins. J Biol Chem, 262(13):6197-6203
    Hadari Y R, Arbel-Goren R, Levy Y, et al. 2000. Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis. J Cell Sci, 113(13):2385-2397
    Hirabayashi J, Kasai K I. 1993. The family of metazoan metal-independent β-galactoside-binding lectins:structure, function and molecular evolution. Glycobiology, 3(4):297-304
    Kasai K I, Hirabayashi J. 1996. Galectins:a family of animal lectins that decipher glycocodes. J Biochem, 119(1):1-8
    Kim H J, Do I G, Jeon H K, et al. 2013. Galectin 1 expression is associated with tumor invasion and metastasis in stage IB to ⅡA cervical cancer. Hum Pathol, 44(1):62-68
    Liu Guangyang, Xu Yi, Li Yan, et al. 2013. Secreted galectin-3 as a possible biomarker for the immunomodulatory potential of human umbilical cord mesenchymal stromal cells. Cytotherapy, 15(10):1208-1217
    Mercy S P D, Ravindranath M H. 1994. Hemolysis and clearance of erythrocytes in Scylla serrata are related to the agglutination by the native sialic acid-specific lectin. Comp Biochem Physiol A, 109(4):1075-1083
    Molchanova V, Chikalovets I, Chernikov O, et al. 2007. A new lectin from the sea worm Serpula vermicularis:isolation, characterization and anti-HIV activity. Comp Biochem Physiol C, 145(2):184-193
    Nair S V, Pearce S, Green P L, et al. 2000. A collectin-like protein from tunicates. Comp Biochem Physiol B, 125(2):279-289
    Nakahara S, Oka N, Raz A. 2005. On the role of galectin-3 in cancer apoptosis. Apoptosis, 10(2):267-275
    Oliveira C, Nicolau A, Teixeira J A, et al. 2011. Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding lectin, on HeLa cervical cancer cells. J Biomed Biotechnol, 2011:568932
    Renwrantz L, Stahmer A. 1983. Opsonizing properties of an isolated hemolymph agglutinin and demonstration of lectin-like recognition molecules at the surface of hemocytes from Mytilus edulis. J Comp Physiol, 149(4):535-546
    Sacchettini J C, Baum L G, Brewer C F. 2001. Multivalent protein-carbohydrate interactions. A new paradigm for supermolecular assembly and signal transduction. Biochemistry, 40(10):3009-3015
    Suh-Chae Y A, Jeune-Chung K H, Chung S R. 1988. Lectins from marine snails:(VⅢ) characterization of the NIC lectin isolated and purified from Neptunea intersculpta. Korean Biochem J, 21:46-52
    Takahashi Y, Itami T, Kondo M. 1995. Immunodefense system of Crustacea. Fish Pathol, 30(2):141-150
    Tunkijjanukij S, Olafsen J A. 1998. Sialic acid-binding lectin with antibacterial activity from the horse mussel:further characterization and immunolocalization. Dev Comp Immunol, 22(2):139-150
    Urcuyo I A, Massoth G J, Julian D, et al. 2003. Habitat, growth and physiological ecology of a basaltic community of Ridgeia piscesae from the Juan de Fuca Ridge. Deep Sea Res I, 50(6):763-780
    Wang Jianhua, Kong Jing, Li Wei, et al. 2006. A β-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity. Comp Biochem Physiol C, 142(1-2):111-117
    Yan Qiaojuan, Li Yanxia, Jiang Zhengqiang, et al. 2009. Antiproliferation and apoptosis of human tumor cell lines by a lectin (AMML) of Astragalus mongholicus. Phytomedicine, 16(6-7):586-593.
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  • 收稿日期:  2016-03-03
  • 修回日期:  2016-08-03

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