Analyzing biases of nitrogen contents and δ<sup>15</sup>N values arising from acidified marine sediments with different CaCO<sub>3</sub> concentrations

PENG Yajun; LIU Dongyan; WANG Yujue; RICHARD Pierre; KEESING John K.

doi:10.1007/s13131-018-1188-2

酸化过程对不同碳酸钙含量的海洋沉积物中总氮及其同位素分析的影响

doi: 10.1007/s13131-018-1188-2

1.
中国科学院烟台海岸带研究所, 海岸带环境过程与生态修复重点实验室, 中国, 烟台 264003;贵州财经大学, 管科学院, 中国, 贵阳 550000
2.
中国科学院烟台海岸带研究所, 海岸带环境过程与生态修复重点实验室, 中国, 烟台 264003;华东师范大学, 河口海岸国家重点实验室, 中国, 上海 200062
3.
中国科学院烟台海岸带研究所, 海岸带环境过程与生态修复重点实验室, 中国, 烟台 264003
4.
拉罗谢尔大学, 海岸、环境与社会研究所, 法国, 拉罗谢尔 17000
5.
西澳大利亚大学, 海洋研究所与印度洋研究中心, 澳大利亚, 克劳利 6009

基金项目: The National Natural Science Foundation of China under contract No. 41376121; the Strategic Priority Research Program of the Chinese Academy of Sciences under contract No. XDA11020405; the Natural Science Foundation of Shandong Province under contract No. JQ201414; the Program of Advanced Talents of The Guizhou University of Finance and Economics, 2016.

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- 收稿日期: 2017-05-26
- 修回日期: 2017-08-30

Analyzing biases of nitrogen contents and δ¹⁵N values arising from acidified marine sediments with different CaCO₃ concentrations

1.
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;School of Management Science, Guizhou University of Finance and Economics, Guiyang 550000, China
2.
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China
3.
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
4.
Littoral, Environment et Sociétés, UMR 7266 CNRS-Université de La Rochelle, 2 rue Olympe de Gouges, La Rochelle 17000, France
5.
CSIRO Oceans and Atmosphere and University of Western Australia Oceans Institute, Indian Ocean Marine Research Centre, Crawley 6009, Australia

摘要

摘要: 为节约成本和样品，一些学者同时分析海洋沉积物中的碳、氮及其同位素（TOC、TN、δ¹³C和δ¹⁵N）。分析沉积物中的δ¹³C，需要对样品进行酸化去除无机碳，但是这一酸化过程会使TN和δ¹⁵N的分析结果产生偏差，且偏差范围与沉积物中无机碳含量（CaCO₃）有关。本研究选取了低CaCO₃含量（1-16%）和高CaCO₃含量（20-40%）的海洋沉积物样品，比较了酸化过程对TN和δ¹⁵N的影响。研究结果表明，酸化过程对海洋沉积物中TN和δ¹⁵N的分析结果产生了显著影响。对于低CaCO₃含量的样品，酸化导致样品中TN流失了约0-40%，δ¹⁵N偏移了约0-2‰；而对于高CaCO₃含量的样品，酸化导致样品中TN流失了约10-60%，δ¹⁵N偏移了约1-14‰。表明酸化对TN和δ¹⁵N的影响已经超过了仪器的误差范围0.002%（TN）和0.08‰（δ¹⁵N），将影响TN和δ¹⁵N的环境指示意义。因此，即使海洋沉积物样品中CaCO₃含量很低，也必须用原样分析TN和δ¹⁵N以避免酸化过程的影响。
- 氮稳定同位素 /
- 总氮 /
- CaCO₃ /
- 酸化 /
- 海洋沉积物
Abstract: In some studies, the researchers pretreated and measured organic carbon, nitrogen and their isotopes (δ¹³C and δ¹⁵N) of marine sediment together, to save costs and resources of analysis. However, the procedure of acidification to remove inorganic carbon for analysing δ¹³C can affect the values of nitrogen and δ¹⁵N, and the biases vary a lot depending on the CaCO₃ contents of sediments. In this study, the biases of total nitrogen (TN) and δ¹⁵N values arising from acidified sediments were compared between the CaCO₃-poor (1%-16%) and CaCO₃-rich (20%-40%) samples. TN and δ¹⁵N values were altered during acid treatment (without centrifugation) that possibly led to N-containing compounds volatilization. For CaCO₃-poor samples, acidification led to a range of 0%-40% TN losses and 0‰-2‰ shift in δ¹⁵N values; and 10%-60% TN losses and 1‰-14‰ shift in δ¹⁵N values for CaCO₃-rich samples. The biases from most samples exceeded the precision of the instrument (0.002% for TN and 0.08‰ for δ¹⁵N), and high biases could mislead our judgment for the environmental implication of the data. Thus, avoiding co-analysis of organic carbon, nitrogen and their isotopes (δ¹³C and δ¹⁵N) in sediments, even for CaCO₃-poor marine sediments, is necessary.
- stable isotope nitrogen /
- nitrogen /
- CaCO₃ /
- acidification /
- marine sediment

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参考文献(22)

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酸化过程对不同碳酸钙含量的海洋沉积物中总氮及其同位素分析的影响

doi: 10.1007/s13131-018-1188-2

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Analyzing biases of nitrogen contents and δ15N values arising from acidified marine sediments with different CaCO3 concentrations

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Analyzing biases of nitrogen contents and δ¹⁵N values arising from acidified marine sediments with different CaCO₃ concentrations