The first record of Pavlova pinguis (Pavlovophyceae, Haptophyta) in China seas
-
摘要: 2015年5月,我们从天津近海分离出一株黄绿色具鞭毛的单细胞藻。这珠藻细胞形态不规则,从圆形到细长型都有出现。绝大多数细胞具有很强的运动能力,一些细胞也可以形成静止的群体。结合形态学、超显微结构观察以及分子生物学分析,我们将这株藻鉴定为定鞭藻门,巴夫藻纲的膨胀巴夫藻。在此之前,中国近海只有绿色巴夫藻的记录,绿色巴夫藻对于水产养殖起到了很重要的作用。巴夫藻纲的其他物种尚无中国海记录。这是膨胀巴夫藻在中国海的首次记录。Abstract: One strain of unicellular flagellated yellow-green algae was successfully isolated from the coastal area near Tianjin in the Bohai Sea in May 2015. The strain ranged from round to elongated in shape. Most of the cells possessed active motility, and some cells formed non-motile aggregation. Based on evidences from morphology, ultrastructure and molecular analysis, we identified the strain as Pavlova pinguis which belonged to Pavlovophyceae, Haptophyta. For Pavlovophyceae, only Pavlova viridis (Diacronema viridis) was reported in China seas prior to this study and it played an important role in aquaculture. This is the first record of Pavlova pinguis in Chinese waters.
-
Key words:
- Pavlova /
- Pavlovophyceae /
- Haptophyta /
- morphology /
- 18S rRNA gene /
- China seas
-
Bendif E M, Probert I, Hervé A, et al. 2011. Integrative taxonomy of the Pavlovophyceae (Haptophyta): a reassessment. Protist, 162(5): 738–761, doi: 10.1016/j.protis.2011.05.001 Brown M R, McCausland M A, Kowalski K. 1998. The nutritional value of four Australian microalgal strains fed to Pacific oyster Crassostrea gigas spat. Aquaculture, 165(3–4): 281–293, doi: 10.1016/S0044-8486(98)00256-7 Brown M R, Mular M, Miller I, et al. 1999. The vitamin content of microalgae used in aquaculture. Journal of Applied Phycology, 11(3): 247–255, doi: 10.1023/A:1008075903578 Butcher R W. 1952. Contributions to our knowledge of the smaller marine algae. Journal of the Marine Biological Association of the United Kingdom, 31(1): 175–191, doi: 10.1017/S0025315400003751 Cavalier-Smith T. 1986. The Kingdom Chromista: origin and systematics. Progress in Phycological Research, 4: 309–347 Cavalier-Smith T. 1989. The Kingdom Chromista. In: The Chromophyte algae: Problems and Perspectives. Oxford: Clarendon, 381–407 Cavalier-Smith T. 1993. Kingdom Protozoa and its 18 phyla. Microbiological Reviews, 57(4): 953–994 Cavalier-Smith T, Allsopp M T E P, Häuber M M, et al. 1996. Chromobiote phylogeny: the enigmatic alga Reticulosphaera japonensis is an aberrant haptophyte, not a heterokont. European Journal of Phycology, 31(3): 255–263, doi: 10.1080/09670269600651461 Chen Jiaofen, He Yichao, Tan Guiying, et al. 1985. Two newly isolated marine chrysophyta and its food values to Mytilus edulis larva. Transaction of Oceanology and Limnology (in Chinese), (2): 44–46 Chrétiennot-Dinet M J, Sournia A, Ricard M, et al. 1993. A classification of the marine phytoplankton of the world from class to genus. Phycologia, 32(3): 159–179, doi: 10.2216/i0031-8884-32-3-159.1 Christensen T A. 1980. Algae. A Taxonomic Survey. Odense: AiO Trykas, 216 Edvardsen B, Eikrem W, Green J C, et al. 2000. Phylogenetic reconstructions of the Haptophyta inferred from 18S ribosomal DNA sequences and available morphological data. Phycologia, 39(1): 19–35, doi: 10.2216/i0031-8884-39-1-19.1 Edvardsen B, Medlin L K. 2007. Molecular systematics of Haptophyta. In: Lewis J, Brodie J, eds. Unravelling the Algae–the Past, Present and Future of Algal Molecular Systematics. Boca Raton, Florida: CRC Press, 183–196 Gayral P. 1980. Particularités et intérêt subséquent de la famille des Pavlovophycées (Chrysophycophytes, Prymnesiophycées). 105e Congrès national des Sociétés savantes Ⅲ: 201–212 Green J C. 1973. Studies in the fine structure and taxonomy of flagellates in the genus Pavlova: Ⅱ. A freshwater representative, Pavlova granifera (Mack) comb. nov. British Phycological Journal, 8(1): 1–12, doi: 10.1080/00071617300650011 Green J C. 1975. The fine-structure and taxonomy of the haptophycean flagellate Pavlova lutheri (Droop) comb. nov. (= Monochrysis lutheri Droop). Journal of the Marine Biological Association of the United Kingdom, 55(4): 785–793, doi: 10.1017/S0025315400017707 Green J C. 1976. Notes on the flagellar apparatus and taxonomy of Pavlova mesolychnon Van der Veer, and on the status of Pavlova Butcher and related genera within the Haptophyceae. Journal of the Marine Biological Association of the United Kingdom, 56(3): 595–602, doi: 10.1017/S0025315400020671 Green J C, Hori T. 1994. Flagella and flagellar roots. In: Green J C, Leadbeater B S C, eds. The Haptophyte Algae. The Systematics Association Special Volume 51. Oxford: Oxford University Press, 47–71 Guillard R R L, Ryther J H. 1962. Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt, and Detonula confervacea (CLEVE) Gran. Canadian Journal of Microbiology, 8(2): 229–239, doi: 10.1139/m62-029 Hagino K, Young J R. 2015. Biology and paleontology of Coccolithophores (Haptophytes). In: Ohtsuka S, Suzaki T, Horiguchi T, et al, eds. Marine Protists. Tokyo: Springer, 311–330 Hibberd D J. 1976. The ultrastructure and taxonomy of the Chrysophyceae and Prymnesiophyceae (Haptophyceae): a survey with some new observations on the ultrastructure of the Chrysophyceae. Botanical Journal of the Linnean Society, 72(2): 55–80, doi: 10.1111/boj.1976.72.issue-2 Holligan P M, Viollier M, Harbour D S, et al. 1983. Satellite and ship studies of coccolithophore production along a continental shelf edge. Nature, 304(5924): 339–342, doi: 10.1038/304339a0 Jordan R W, Kleijne A. 1994. A classification system for living coccolithophores. In: Winter A, Siesser W G, eds. Coccolithophores. Cambridge: Cambridge University Press, 83–105 Liu Dongchao, Liang Feilong. 1999. The feeding effects of Pavlova viridis to Brachionus plicatilis. Journal of Fujian Fisheries (in Chinese), (1): 9–13 Liu Luwei. 1995. Studies on nutrient requirements of Pavlova viridis and its productive culture. Journal of Hebei Fisheries (in Chinese), (5): 3–7 Lu Jun, Zhang Daonan. 1994. Pavlova viridis as bait for artificial propagation of Macrobrachium rosenbergii in imitating ecological system. Journal of Shanghai Fisheries University (in Chinese), 3(1): 63–68 McCausland M A, Brown M R, Barrett S M, et al. 1999. Evaluation of live microalgae and microalgal pastes as supplementary food for juvenile Pacific oysters (Crassostrea gigas). Aquaculture, 174(3–4): 323–342, doi: 10.1016/S0044-8486(99)00018-6 Medlin L, Elwood H J, Stickel S, et al. 1988. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71(2): 491–499, doi: 10.1016/0378-1119(88)90066-2 Medlin L K, Sáez A G, Young J R. 2008. A molecular clock for coccolithophores and implications for selectivity of phytoplankton extinctions across the K/T boundary. Marine Micropaleontology, 67(1–2): 69–86, doi: 10.1016/j.marmicro.2007.08.007 Meireles L A, Guedes A C, Malcata F X. 2003. Lipid class composition of the microalga Pavlova lutheri: Eicosapentaenoic and docosahexaenoic acids. Journal of Agricultural and Food Chemistry, 51(8): 2237–2241, doi: 10.1021/jf025952y Oviedo A, Ziveri P, Álvarez M, et al. 2015. Is coccolithophore distribution in the Mediterranean Sea related to seawater carbonate chemistry?. Ocean Science, 11(1): 13–32, doi: 10.5194/os-11-13-2015 Ponis E, Probert I, Véron B, et al. 2006. Nutritional value of six Pavlovophyceae for Crassostrea gigas and Pecten maximus larvae. Aquaculture, 254(1–4): 544–553, doi: 10.1016/j.aquaculture.2005.11.017 Sadeghi A, Dinter T, Vountas M, et al. 2012. Remote sensing of coccolithophore blooms in selected oceanic regions using the PhytoDOAS method applied to hyper-spectral satellite data. Biogeosciences, 9(6): 2127–2143, doi: 10.5194/bg-9-2127-2012 Sun Jun, Gu Xiaoyao, Feng Yuanyuan, et al. 2014. Summer and winter living coccolithophores in the Yellow Sea and the East China Sea. Biogeosciences, 11(3): 779–806, doi: 10.5194/bg-11-779-2014 Spurr A R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research, 26(1–2): 31–43, doi: 10.1016/S0022-5320(69)90033-1 Tseng C K, Chen Jiaofen, Zhang Zhefu. 1992. On a new species of Pavlova (Prymnesiophyceae) from China. Chinese Journal of Oceanology and Limnology, 10(1): 23–30, doi: 10.1007/BF02844296 Zhao Sufen, Sun Huiqiang. 2004. Studies on the ecological factors to the growth of Pavlova viridis. Fisheries Science (in Chinese), 23(2): 9–11
点击查看大图
计量
- 文章访问数: 535
- HTML全文浏览量: 21
- PDF下载量: 488
- 被引次数: 0