Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea

Yang Jinyu Feng Yangyang Chen Xiulan Xie Binbin Zhang Yuzhong Shi Mei Zhang Xiying

杨金玉, 冯阳阳, 陈秀兰, 解彬彬, 张玉忠, 石梅, 张熙颖. 南海沉积物细菌胞外蛋白酶在家族水平上的多样性[J]. 海洋学报英文版, 2019, 38(12): 73-83. doi: 10.1007/s13131-019-1391-9
引用本文: 杨金玉, 冯阳阳, 陈秀兰, 解彬彬, 张玉忠, 石梅, 张熙颖. 南海沉积物细菌胞外蛋白酶在家族水平上的多样性[J]. 海洋学报英文版, 2019, 38(12): 73-83. doi: 10.1007/s13131-019-1391-9
Yang Jinyu, Feng Yangyang, Chen Xiulan, Xie Binbin, Zhang Yuzhong, Shi Mei, Zhang Xiying. Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea[J]. Acta Oceanologica Sinica, 2019, 38(12): 73-83. doi: 10.1007/s13131-019-1391-9
Citation: Yang Jinyu, Feng Yangyang, Chen Xiulan, Xie Binbin, Zhang Yuzhong, Shi Mei, Zhang Xiying. Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea[J]. Acta Oceanologica Sinica, 2019, 38(12): 73-83. doi: 10.1007/s13131-019-1391-9

南海沉积物细菌胞外蛋白酶在家族水平上的多样性

doi: 10.1007/s13131-019-1391-9
基金项目: The AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology under contract No. 2017ASTCP-OS14; the National Natural Science Foundation of China under contract Nos 31670063, 31670497 and 31870052; the Taishan Scholars Program of Shandong Province under contract No. 2009TS079; the Science and Technology Basic Resources Investigation Program of China under contract No. 2017FY100804.

Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea

  • 摘要: 海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。
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