Genetic parameters estimation for growth traits in cultured tiger pufferfish (fugu), Takifugu rubripes
-
Abstract: The aim of this study was to evaluate the genetic parameters of the growth performance of Takifugu rubripes. Heritabilities and genetic correlations were estimated for body weight (BW), body length (BL), total length (TL), chest measurement (CM) and trunk length (TKL) of T. rubripes from measurements of progeny at 6 months and 12 months. The results showed that the heritability was 0.37 for BW6, 0.19 for BL6, 0.35 for TL6, 0.29 for CM6, 0.26 for TKL6, 0.36 for BW12, 0.26 for BL12, 0.25 for TL12, 0.31 for CM12 and 0.15 for TKL12. The range of genetic correlations estimated at 6 months was 0.025–0.725 and −0.002–0.706 at 12 months. The results indicated that genetic improvement for faster growth rate or increased body weight in cultured T. rubripes was effective. Based on selection theory, the selection strategy for traits with medium heritability is flexible. Considering that these growth traits do not reach the high level of heritability, family selection should be expected. Given positive genetic correlations among BW, BL, TL, CM, and TKL at 6 months, the five traits could be improved simultaneously through selective breeding. As there was high genetic correlation only between BW12, BL12 and TL12, and negative correlations between TKL12 and BL12 as well as between CM12 and TL12, and only BW, BL and TL at 12 months could be improved simultaneously.
-
Key words:
- family /
- genetic evaluation /
- heritability /
- genetic correlation
-
Figure 1. Morphometric measurements of Takifugu rubripes. BL: body length; CM: chest measurement; TKL: trunk length; TL: total length.
Figure 2. Forty eight full-sib families’ growth tendency in terms of body weight (BW) in the 0−12-month stage.
Figure 3. Forty eight full-sib families’ growth tendency in terms of body length (BL) in the 0−12-month stage.
Figure 4. Forty eight full-sib families’ growth tendency in terms of total length (TL) in the 0−12-month stage.
Figure 5. Forty eight full-sib families’ growth tendency in terms of chest measurement (CM) in the 0−12-month stage.
Figure 6. Forty eight full-sib families’ growth tendency in terms of trunk length (TKL) in the 0−12-month stage.
Table 1. Descriptive statistics for all the variables included in the analysis
Trait Number of
Takifugu rubripesMean trait
valueStandard
deviationCoefficient of
variation/%Minimum
trait valueMaximum
trait valueBW6 2 400 167.474 g 32.220 g 19.238 93.500 g 216.700 g BL6 2 400 16.069 cm 1.144 cm 8.986 14.600 cm 19.200 cm TL6 2 400 19.925 cm 1.176 cm 5.902 17.000 cm 21.300 cm CM6 2 400 14.481 cm 1.408 cm 9.723 11.000 cm 16.200 cm TKL6 2 400 5.908 cm 0.772 cm 13.067 5.000 cm 7.000 cm BW12 2 011 353.379 g 62.369 g 17.649 176.000 g 513.700 g BL12 2 011 20.625 cm 1.235 cm 5.987 17.900 cm 24.300 cm TL12 2 011 23.957 cm 1.354 cm 5.651 19.600 cm 27.000 cm CM12 2 011 19.479 cm 1.554 cm 7.977 18.000 cm 22.600 cm TKL12 2 011 7.002 cm 0.783 cm 11.182 6.000 cm 8.800 cm Note: BW, body weight; BL, body length; TL, total length; CM, chest measurement; TKL, trunk length. 
下载: 导出CSV
Table 2. Variance components and heritability (h2) of five growth traits in Takifugu rubripes
Growth traits Variance components $ {{\sigma }}_{\mathrm{a}}^{2} $ ${\sigma }_{{\rm{f}}}^{2}$ ${\sigma }_{{\rm{e}}}^{2}$ h2 BW6 265.967 9 g 360.891 0 g 95.155 0 g 0.37±0.040 BL6 0.102 2 cm 0.265 6 cm 0.172 8 cm 0.19±0.023 TL6 0.580 6 cm 0.820 8 cm 0.274 0 cm 0.35±0.037 CM6 0.288 1 cm 0.487 0 cm 0.216 9 cm 0.29±0.032 TKL6 0.092 0 cm 0.170 5 cm 0.092 1 cm 0.26±0.026 BW12 345.567 4 g 524.396 0 g 96.666 0 g 0.36±0.041 BL12 0.413 9 cm 0.824 89 cm 0.341 9 cm 0.26±0.031 TL12 0.485 7 cm 0.959 3 cm 0.535 0 cm 0.25±0.025 CM12 0.519 0 cm 0.869 2 cm 0.311 0 cm 0.31±0.034 TKL12 0.093 1 cm 0.305 8 cm 0.242 8 cm 0.15±0.020 Note: BW6, 6-month body weight; BL6, 6-month body length; TL6, 6-month total length; CM6, 6-month chest measurement; TKL6, 6-month trunk length; BW12, 12-month body weight; BL12, 12-month body length; TL12, 12-month total length; CM12, 12-month chest measurement; TKL12, 12-month trunk length; ${\sigma }_{{\rm{a}}}^{2}$, additive genetic variance; ${\sigma }_{{\rm{f}}}^{2}$, full-sib variance; ${\sigma }_{{\rm{e}}}^{2}$, residual variance; h2, heritability.
下载: 导出CSV
Table 3. Phenotypic (rp) (above the diagonal) and genetic (under the diagonal) correlations (rg) of growth traits in 6-month-old Takifugu rubripes
Trait BW6 BL6 TL6 CM6 TKL6 BW6 0.727** 0.727** 0.580** 0.155** BL6 0.725** 0.454** 0.461** 0.010 TL6 0.721** 0.445** 0.438** 0.105** CM6 0.578** 0.464** 0.420** 0.344** TKL6 0.146** 0.025 0.074** 0.357** Note: BW6, 6-month body weight; BL6, 6-month body length; TL6, 6-month total length; CM6, 6-month chest measurement; TKL6, 6-month trunk length. ** Correlation is significant at the 0.01 level (2-tailed).
下载: 导出CSV
Table 4. Phenotypic (rp) (above the diagonal) and genetic (under the diagonal) correlations (rg) of growth traits in 12-month-old Takifugu rubripes
Trait BW12 BL12 TL12 CM12 TKL12 BW12 0.145** 0.482** 0.581** 0.697** BL12 0.146** 0.462** 0.075** 0.001 TL12 0.495** 0.466** 0.026 0.453** CM12 0.547** 0.072** −0.020 0.376** TKL12 0.706** −0.002 0.453** 0.373** Note: BW12, 12-month body weight; BL12, 12-month body length; TL12, 12-month total length; CM12, 12-month chest measurement; TKL12, 12-month trunk length. ** Correlation is significant at the 0.01 level (2-tailed).
下载: 导出CSV
-
Boldman K G, Kriese L A, Van Vleck L D, et al. 1995. A manual for use of MTDFREML—A set of programs to obtain estimates of variances and covariances (draft). Clay Center: U. S. Department of Agriculture-Agricultural Research Service Cardellino R, Rovira J. 1987. Mejoramiento Genético Animal (in Spanish). Buenos Aires: Hemisferio Sur, 253 Cui Jianzhou, Shen Xueyan, Gong Qingli, et al. 2006. Identification of sex markers by cDNA-AFLP in Takifugu rubripes. Aquaculture, 257(1–4): 30–36, Fu Jianjun, Shen Yubang, Xu Xiaoyan, et al. 2015. Genetic parameter estimates and genotype by environment interaction analyses for early growth traits in grass carp (Ctenopharyngodon idella). Aquaculture International, 23(6): 1427–1441. doi: 10.1007/s10499-015-9894-7 Li Weiye, Ma Aijun, Wang Xin’an, et al. 2013. The study of Takifugu rubripes breeding family establishment and the early standardized cultivation technique. Oceanologia et Limnologia Sinica, 44(4): 944–952 Lin Zijie, Hosoya S, Sato M, et al. 2020. Genomic selection for heterobothriosis resistance concurrent with body size in the tiger pufferfish, Takifugu rubripes. Scientific Reports, 10(1): 19976. doi: 10.1038/s41598-020-77069-z Liu Feng, Li Yangzhen, Wang Xiaoxia, et al. 2016. Estimation of genetic parameters for disease-resistance traits in Cynoglossus semilaevis (Günther, 1873). Journal of Applied Ichthyology, 32(4): 643–651. doi: 10.1111/jai.13067 Liu Xiande, Sui Banliang, Wang Zhiyong, et al. 2011. Estimated reproductive success of brooders and heritability of growth traits for large yellow croaker (Larimichthys crocea) using microsatellites. Chinese Journal of Oceanology and Limnology, 29(5): 990–995. doi: 10.1007/s00343-011-0266-5 Liu Yongxin, Zhou Qin, Zhang Hongtao, et al. 2014. Estimation of genetic parameters of growth traits of redfin puffer (Takifugu rubripes). Progress in Fishery Sciences, 35(6): 39–44. doi: 10.11758/yykxjz.20140606 Liu Yongxin, Zhou Qin, Zhang Hongtao, et al. 2017. Genetic analysis of redfin puffer (Takifugu rubripes) for growth traits by using a marker-based pedigree. Journal of Fisheries of China, 41(1): 21–30. doi: 10.11964/jfc.20160210282 Ma Aijun, Wang Xin’an, Huang Zhihui, et al. 2018. Estimation of genetic parameters for upper thermal tolerance and growth-related traits in turbot Scophthalmus maximus using the Bayesian method based on Gibbs sampling. Acta Oceanologica Sinica, 37(6): 40–46. doi: 10.1007/s13131-018-1185-5 Ma Aijun, Wang Xin’an, Sun Zhibin, et al. 2016. Morphological variations among three different populations of Takifugu rubripes. Oceanologia et Limnologia Sinica, 47(1): 166–172. doi: 10.11693/hyhz20150300083 Norušis M J. 2005. SPSS 13.0 Guide to Data Analysis. Upper Saddle River: Prentice Hall Perry G M L, Martyniuk C M, Ferguson M M, et al. 2005. Genetic parameters for upper thermal tolerance and growth-related traits in rainbow trout (Oncorhynchus mykiss). Aquaculture, 250(1–2): 120–128, Srimai W, Koonawootrittriron S, Manee-Aphai W, et al. 2019. Genetic parameters and genotype-environment interaction for growth traits of North African catfish, Clarias gariepinus (Burchell, 1822). Aquaculture, 501: 104–110. doi: 10.1016/j.aquaculture.2018.10.064 Sun Miaomiao, Huang Jianhua, Jiang Shigui, et al. 2015. Estimates of heritability and genetic correlations for growth-related traits in the tiger prawn Penaeus monodon. Aquaculture Research, 46(6): 1363–1368. doi: 10.1111/are.12290 Taylor R S, Kube P D, Muller W J, et al. 2009. Genetic variation of gross gill pathology and survival of Atlantic salmon (Salmo salar L.) during natural amoebic gill disease challenge. Aquaculture, 294(3–4): 172–179, Wang Hongxia, Chai Xueliang, Liu Baozhong. 2011. Estimation of genetic parameters for growth traits in cultured clam Meretrix meretrix (Bivalvia: Veneridae) using the Bayesian method based on Gibbs sampling. Aquaculture Research, 42(2): 240–247. doi: 10.1111/j.1365-2109.2010.02617.x Wang Xin’an, Ma Aijin. 2019. Genetic parameters for resistance against Vibrio anguillarum in turbot Scophthalmus maximus. Journal of Fish Diseases, 42(5): 713–720. doi: 10.1111/jfd.12973 Wang Xin’an, Ma Aijun. 2020. Dynamic genetic analysis for body weight and main length ratio in turbot Scophthalmus maximus. Acta Oceanologica Sinica, 39(2): 22–27. doi: 10.1007/s13131-020-1551-y Wang Xin’an, Ma Aijun, Huang Zhihui, et al. 2019. Estimation of genetic parameters for upper thermal tolerances and growth-related traits in turbot Scophthalmus maximus. Journal of Oceanology and Limnology, 37(5): 1736–1745. doi: 10.1007/s00343-019-7267-1 Wang Xin’an, Ma Aijun, Sun Zhibin. 2021. Genetic parameters of seven immune factors in turbot (Scophthalmus maximus) infected with Vibrio anguillarum. Journal of Fish Diseases, 44(3): 263–271. doi: 10.1111/jfd.13320 Yu Hailong, Gu Chenlei, Kong Derong, et al. 2016. Heritability estimating and correlativity of growth traits in redfin puffer Takifugu rubripes. Journal of Dalian Ocean University, 31(4): 357–361. doi: 10.16535/j.cnki.dlhyxb.2016.04.002 -
点击查看大图
图(6) / 表(4)
计量
- 文章访问数: 434
- HTML全文浏览量: 145
- PDF下载量: 18
- 被引次数: 0
