On four closely related hypotrichous ciliates (Protozoa, Ciliophora, Spirotrichea): molecular characters, interspecific relationships and phylogeny defined with multigene sequence information

GAO Feng; YI Zhenzhen; CHEN Zigui; AL-RASHEID Khaled A S; SONG Weibo

doi:10.1007/s13131-010-0067-2

Article Contents

Article Navigation > Acta Oceanologica Sinica > 2010 > 29(5): 90-96

GAO Feng, YI Zhenzhen, CHEN Zigui, AL-RASHEID Khaled A S, SONG Weibo. On four closely related hypotrichous ciliates (Protozoa, Ciliophora, Spirotrichea): molecular characters, interspecific relationships and phylogeny defined with multigene sequence information[J]. Acta Oceanologica Sinica, 2010, 29(5): 90-96. doi: 10.1007/s13131-010-0067-2

Citation:

GAO Feng, YI Zhenzhen, CHEN Zigui, AL-RASHEID Khaled A S, SONG Weibo. On four closely related hypotrichous ciliates (Protozoa, Ciliophora, Spirotrichea): molecular characters, interspecific relationships and phylogeny defined with multigene sequence information[J]. Acta Oceanologica Sinica, 2010, 29(5): 90-96. doi: 10.1007/s13131-010-0067-2

Citation:

GAO Feng, YI Zhenzhen, CHEN Zigui, AL-RASHEID Khaled A S, SONG Weibo. On four closely related hypotrichous ciliates (Protozoa, Ciliophora, Spirotrichea): molecular characters, interspecific relationships and phylogeny defined with multigene sequence information[J]. Acta Oceanologica Sinica, 2010, 29(5): 90-96. doi: 10.1007/s13131-010-0067-2

PDF( 730 KB)

On four closely related hypotrichous ciliates (Protozoa, Ciliophora, Spirotrichea): molecular characters, interspecific relationships and phylogeny defined with multigene sequence information

doi: 10.1007/s13131-010-0067-2

1.
Laboratory of Protozoology, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou 510631, China;Laboratory of Protozoology, KLM, Ocean University of China, Qingdao 266003, China
2.
Laboratory of Protozoology, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou 510631, China
3.
Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia

Received Date: 2009-05-22
Rev Recd Date: 2009-12-14

Abstract

Abstract

In order to clarify the phylogeny and relationships of the most confused hypotrichous ciliates, Holosticha-complex, four closely related holostichids (five populations), Holosticha bradburyae, H. diademata, Anteholosticha sp., and A. manca, were compared and analyzed using ITS2 secondary structures, ITS1-5.8S-ITS2 region and SSrRNA gene sequences. The ITS1-5.8S-ITS2 region sequences of these four species were first sequenced, and they shared sequence identities ranging from 68.0% to 90.1%, while two populations of Anteholosticha sp. differed in three nucleotides (sequence identity 99.8%). There were several minor differences among ITS2 secondary structures of these species, while two populations of Anteholosticha sp. had the identical secondary structure. Phylogenetic trees inferred from the ITS1-5.8S-ITS2 region sequences of stichotrichs using multiple algorithms (Neighbor-Joining, Maximum Parsimony and Bayesian) revealed similar topologies. The results show that:(1) Holosticha bradburyae and H. diademata firmly clustered together with strong bootstrap supports, forming a sister clade with Anteholosticha sp., (2) Anteholosticha appeared to be a paraphyletic assemblage, in which the morphotype A. manca was more closely related to Diaxonella trimarginata than to its congener Anteholosticha sp. Phylogenetic analyses based on the SSrRNA gene and the combined sequences of SSrRNA gene and ITS1-5.8S-ITS2 region revealed the similar relationships between Holosticha and Anteholosticha, nevertheless their positions within the subclass Stichotrichia differed from each other inferred from different genes.
- phylogeny,
- gene sequencing,
- marine ciliates,
- SSrRNA,
- ITS1-5.8S-ITS2

FullText(HTML)

References(25)

References

ERICKSON C M. Age determination of Manitoban walleys using otoliths, dorsal spines,and scales[J].North American Journal of Fisheres Management, 1983,112:735—743.

STEVENSON D K, CAMPANA S E. Otolith Microstructure Examination and Analysis[M].Canada:NRC Research Press,1992: 1—126.

CAMPANA S E. Chemistry and composition of otoliths: pathways, mechanisms and applications[J]. Marine Ecology Progress Series,1999,188:263—279.

CAMPANA S E, THORROLD S R. Otoliths, increments and elements: keys to a compressive understanding of fish populations[J]. Canadian Journal of Fisheries and Aquatic Sciences , 2001,58:30—38.

ELSDON T S, GILLANDERS B M. Reconstructing migratory patterns of fish based on environmental influences on otolith chemistry[J]. Reviews in Fish Biology and Fisheries, 2003, 13:219—235.

窦硕增.鱼类的耳石信息分析及生活史重建——理论、方法与应用[J].海洋科学集刊,2007,48:93—113.

CAMPANA S E. Otolith science entering the 21st century[J]. Marine and Freshwater Research,2005,56:485—495.

CAMPANA S E. Photographic Atlas of Fish Otoliths of the Northwest Atlantic Ocean . Canada:NRC Research Press,2004:1-258.

SMALE M J , WATSON G, HECHT T. Otolith Atlas of Southern African Marine Fishes. Grahamstown . South Africa: J. L. B. Smith Institute of Ichthyology,1995: 1—232.

CAMPANA S E.Stock discrimination using otolith shape analysis [J]. Fish Aquat Sci, 1993, 50: 1062—1083.

BEGG G A B, OVERHOLTZ W J, MUNROE N J. The use of internal otolith morphometrics for indentification of haddock (Melanogrammus aeglefinus) stocks on Georges Bank [J]. Fish Bull, 2001, 99: 1—14.

BEGG G A.Stock identification of haddock Melanogrammus aegefinus on Georges Bank base on otolith shape analysis [J].Transactions of the American Fisheries Society, 2000,129:935—945.

TRACEY S R. Application of elliptical Fourier analysis of otolith form as a tool for stock identification[J].Fisheries Research,2006,77:138—147.

VOLPEDO A,ECHEVERRIA D D. Ecomorphological patterns of the sagitta in fish on the continental shelf off Argentine[J]. Fish Res, 60:551—560.

ASSIS C A. The lagenar otoliths of Teleosts : their morphology and its application in species identification , phylogeny and systematic[J]. J Fish Biol ,2003 ,62 :1268—1295.

PIERCE G J ,BOYLE P R. A review of methods for diet analysis in piscivorous marine mammals[J]. Oceanography and Marine Biology,1991,29 :409—486.

MURIE D J, LAVIGNE D M. Interpretation of otoliths in stomach content analysis of phocid seals:quantifying fish consumption[J]. Can J Zool, 1985, 64:1152—1157.

朱元鼎,罗云林,伍汉霖.中国石首鱼类分类系统的研究和新属新种的叙述 //中国鱼类专著集.上海:上海科学技术出版社,1963:12—19.

郑文莲.中国鲹科等鱼类耳石形态的比较研究 //鱼类学论文集:第2辑.北京:科学出版社,1981:39—54.

叶振江,孟晓梦,高天翔,等.两种花鲈耳石形态的地理差异[J].海洋与湖沼,2007,38(4):356—360.

叶振江,朱柏军,薛莹.中国习见海洋鱼类耳石图谱:第1辑.青岛:中国海洋大学出版社,2007.231.

张晓霞,叶振江,王英俊,等.青岛海域绿鳍鱼耳石形态的初步研究[J].中国海洋大学学报, 2008, 39(4):622—626.

张波,戴芳群,金显仕.黄海重要饵料鱼种矢耳石的形态特征[J].2008,15(6): 917—926.

郭弘艺,唐文乔,魏凯,等.长江口鲚属鱼类的矢耳石形态特征[J].动物学杂志,2007,42(1):39—47.

刘瑞玉.中国海洋生物名录 .北京,科学出版社,2008:974—976.

Relative Articles

Supplements(0)

Cited By

Proportional views