Distribution and germination of viable diatom resting stage cells in sediments of the East China Sea

ZHANG Yuyu; LU Songhui; ZHANG Chuansong; GAO Yahui

doi:10.1007/s13131-010-0070-7

Article Contents

Article Navigation > Acta Oceanologica Sinica > 2010 > 29(5): 121-128

ZHANG Yuyu, LU Songhui, ZHANG Chuansong, GAO Yahui. Distribution and germination of viable diatom resting stage cells in sediments of the East China Sea[J]. Acta Oceanologica Sinica, 2010, 29(5): 121-128. doi: 10.1007/s13131-010-0070-7

Citation:

ZHANG Yuyu, LU Songhui, ZHANG Chuansong, GAO Yahui. Distribution and germination of viable diatom resting stage cells in sediments of the East China Sea[J]. Acta Oceanologica Sinica, 2010, 29(5): 121-128. doi: 10.1007/s13131-010-0070-7

Citation:

PDF( 911 KB)

Distribution and germination of viable diatom resting stage cells in sediments of the East China Sea

doi: 10.1007/s13131-010-0070-7

1.
Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China
2.
Research Center for Harmful Algae and Aquatic Environment, Jinan University, Guangzhou 510632, China;College of Life Science, South China Normal University, Guangzhou 510631, China
3.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China

Received Date: 2009-07-15
Rev Recd Date: 2009-12-14

Abstract

Abstract

The abundance and temporal patterns of viable diatom resting stage cells in sediments of the East China Sea in 2006 have been investigated. The abundance of viable resting stages was enumerated with the most probable number (MPN) technique. Overall, 25 diatom species (including varieties) belonging to 16 genera were detected. Viable resting stage cells were common, on the order of 10³ to 10⁶ cells/g dry mass. The abundant taxa included Skeletonema marina, S. dohrnii, Chaetoceros curvisetus and Thalassiosira spp., with Skeletonema accounting for 83.3% of the cell. The effects of temperature (10, 15, 20, 25℃), salinity (20, 25, 30, 35 psu) and light intensity (0, 300, 4 000, 8 000 lux) on the germination of resting stages in sediment samples were also determined. The results showed that light intensity had a significant positive effect on the germination of diatom resting stage cells (p <0.01) and that diatoms could not germinate in darkness. Temperature and salinity had no noticeable effects on the germination. The present study suggests that there are abundant diatom resting cells in sediments of the East China Sea, which could serve as a "seed bank" in the phytoplankton population succession and contribute to the initiation of algal blooms.
- diatom,
- germination,
- MPN,
- resting stages,
- seed bank,
- sediment

FullText(HTML)

References(31)

References

鄢庆枇,义家波,陈强,等. 病原性副溶血弧茵在天然海水中的饥饿耐受研究[J]. 海洋学报, 2007,29(3):131—136.

牟海津,李筠,包振民,等. 副溶血弧菌胞外产物对中国对虾的致病性分析[J]. 海洋与湖沼,2000,31(3):273—280.

DANIELS N A,MACKINNON L,BISHOP R,et al. Vibrio parahaemolyticus infections in the United States,1973-1998 [J]. J Infect Dis,2000,181(5): 1661—1666.

KANEKO T,COLWELL R R. Ecology of Vibrio parahaemolyticus in Chesapeake Bay [J]. Journal of Bacteriology,1973,113(1): 24—32.

HACKNEY C R,KLEEMAN E G,RAY B,et al. Adherence as a method of differentiating virulent and avirulent strains of Vibrio parahaemolyticus[J]. Appl Environ Microbiol,1980,40(3): 652—658.

STOODLEY P,SAUER K,DAVIES D G,et al. Biofilms as complex differentiated communities[J]. Annual Review of Biochemistry,2002,56:187—209.

MELULENI G J,GROUT M,EVANS D J,et al. Mucoid Pseudomonas aeruginosa growing in a biofilm in vitro are killed by opsonic antibodies to the mucoid exopolysaccharide capsule but not by antibodies produced during chronic lung infection in cystic fibrosis patients[J]. Immunol,1995,155:2029—2038.

COSTERTON J W,CHENG K J,GEESEY G G,et al. Bacterial biofilms in nature and disease[J]. Annu Rev Microbiol,1987,41: 435—464.

CHICUREL M. Bacterial biofilms and infections. slimebusters[J]. Nature,2000,408(6810): 284—286.

ZHU J,MEKALANOS J J. Quorum sensing-dependent biofilms enhance colonization in Vibrio cholerae[J]. Developmental Cell,2003,5(4): 647—656.

鄢庆枇,王军,苏永全,等. 网箱养殖大黄鱼弧菌病研究[J]. 集美大学学报:自然科学版,2001,6(3):191—196.

PRATT L A,KOTLER R. Genetic analysis of Escherichia coli biofilm formation: roles of flagella,motility,chemotaxis and type I pili[J]. Molecular Microbiology,1998,30(2):285—293.

CHEN Qiang,YAN Qing-pi,WANG Ke-juan,et al. The portal of entry for pathogenic Vibrio alginolyticus into Pseudosciaene crocea and characteristic of the bacterial adhesion to the mucus[J]. Disease of Aquatic Organism,2008,80:181—188.

BRADFORD M M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Annual Biochemistry,1976,72: 248—254.

李京宝. 一种海洋的弧菌胞外多糖A101的抗生物膜作用研究 .青岛:中国海洋大学,2007.

KLAUSEN M,HEYDORN A,RAGAE P,et al. Biofilm formation by Pseudomonas aeruginosa wild type,flagella and type IV pili mutants[J]. Mol Microbiol,2003, 48: 1511—1524.

HOUDT R V,MICHIELS C W. Role of bacterial cell surface structures in Escherichia coli biofilm formation[J]. Research in Microbiology,2005,156:626—633.

PAYNE G A,NYSTROM G J,BHATNAGAR D,et al. Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus[J]. Appl Environ Microbiol,1993,59(1):156—162.

SUTHERLAND I W. Biofilm exopolysaccharides: a strong and sticky framework[J]. Microbiology,2001,147(1): 3—9.

JOHNSON L R. Microcolony and biofilm formation as a survival strategy for bacteria[J]. Journal of Theoretical Biology,2008,251(1): 24—34.

YAN Qing-pi,CHEN Qiang,MA Shen,et al. Characteristics of adherence of pathogenic Vibrio alginolyticus to the intestinal mucus of Large yellow croaker(Pseudosciaena crocea)[J]. Aquaculture,2007,269: 21—30.

ULITZUR S. Effect of temperature,salts,pH and other factors on the development of peritrichous flagella in Vibrio alginolyticus[J]. Arch Microbiol,1975,104:285—288 .

鄢庆枇,陈强,马甡,等. 病原性溶藻弧菌对大黄鱼鳃黏液黏附作用研究[J]. 海洋学报,2007,29(6):80—86.

李涛,宁喜斌.副溶血性弧菌温度-盐度双因素预测模型的建立[J]. 微生物学通报,2009,36(8):1200—1205.

ATSUMI T,MCCARTER L,IMAE Y. Polar and lateral flagellar motors of marine Vibrio are driven by different ion-motive forces[J]. Nature,1992,355:182—184.

刘代新,宁喜斌,张继伦. 响应面分析法优化副溶血性弧菌生长条件[J]. 微生物学通报,2008,35(2):306—310.

KNOX K W,HARDY L N,MARKEVICS L J,et al. Comparative studies on the effect of growth conditions on adhesion,hydrophobicity,and extracellular protein profile of Streptococcus sanguis G9B[J]. Infect Immun,1985,50(2): 545—554.

KIEREK K,WATNICK P I. The Vibrio cholerae O139 O-antigen polysaccharide is essential for Ca²⁺-dependent biofilm development in sea water[J]. PNAS,2003,100(24):14357—14362.

BANIN E,BRADY K M,GREENBERG E P. Chelator-induced dispersal and killing of Pseudomonas aeruginosa cells in a biofilm[J]. Microbiology,2006,72(3): 2064—2069.

DOBSON L F,O'SHEA D G. Antagonistic effect of divalent cations Ca²⁺ and Mg²⁺on the morphological development of Streptomyces hygroscopicus var geldanus[J]. Appl Microbiol Biotechnol,2008,81:119—126.

VILLASEFIOR J C,VAN LOOSDRECHT M C M,PICIOREANU C,et al. Influence of different substrates on the formation of biofilms in a biofilm airlift suspension reactor[J]. Water Science and Technology,2000,41(4):323—330.

Relative Articles

Supplements(0)

Cited By

Proportional views