LIU Yunguo, GUO Yinghui, LIU Shuaishuai, LI Junfeng, YE Naihao. Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers[J]. Acta Oceanologica Sinica, 2011, (3): 76-83. doi: 10.1007/s13131-011-0121-8
Citation:
LIU Yunguo, GUO Yinghui, LIU Shuaishuai, LI Junfeng, YE Naihao. Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers[J]. Acta Oceanologica Sinica, 2011, (3): 76-83. doi: 10.1007/s13131-011-0121-8
LIU Yunguo, GUO Yinghui, LIU Shuaishuai, LI Junfeng, YE Naihao. Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers[J]. Acta Oceanologica Sinica, 2011, (3): 76-83. doi: 10.1007/s13131-011-0121-8
Citation:
LIU Yunguo, GUO Yinghui, LIU Shuaishuai, LI Junfeng, YE Naihao. Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers[J]. Acta Oceanologica Sinica, 2011, (3): 76-83. doi: 10.1007/s13131-011-0121-8
Inspection & Quarantine Technology Center, Shandong Entry-Exit Inspection & Quarantine Bureau, Qingdao 266002, China;Ocean University of China, Qingdao 266003, China
2.
College of Basic Medical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
3.
Ocean University of China, Qingdao 266003, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
4.
College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, China
5.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Ten highly variable microsatellite loci were performed in order to evaluate the genetic variability of wild and hatchery samples of half-smooth tongue sole Cynoglossus semilaevis. A group of 200 genotypes belonging to four wild samples, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ) and one hatchery sample, Mingbo (MB), were screened. All of the ten microsatellite loci screened in this study showed marked polymorphism. A total of 70 different alleles were observed over all loci. The number of alleles per locus ranged from 4.2 to 10.6. The average of expected and observed heterozygosity ranged from 0.67 to 0.82, and from 0.77 to 0.87, respectively. A total of 10, 16, 10, 11 and 5 unique alleles each were found in LZ, WH, QD, RZ and MB populations. The effective number of alleles varied from 2.87 for HSTS_7 to 6.83 for HSTS-h. The number of average genotypes ranged from 6.0 for HSTS_a to 15.4 for HSTS_h. As compared with the wild populations, the hatchery population, MB, showed significant genetic changes such as fewer alleles per locus (P <0.05), a smaller number of low frequency alleles (P <0.05), a small number of unique alleles and a small number of genotypes (P <0.05), all indicative of a reduction in genetic diversity.
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