Characterization of the <i>cyp19a1a</i> gene froma BAC sequence in half-smooth tongue sole (<i>Cynoglossus semilaevis</i>) and analysis of its conservation among teleosts

SHAO Changwei; LIU Geng; LIU Shanshan; LIU Changlin; CHEN Songlin

doi:10.1007/s13131-013-0339-8

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Article Navigation > Acta Oceanologica Sinica > 2013 > 32(8): 35-43

SHAO Changwei, LIU Geng, LIU Shanshan, LIU Changlin, CHEN Songlin. Characterization of the cyp19a1a gene froma BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts[J]. Acta Oceanologica Sinica, 2013, 32(8): 35-43. doi: 10.1007/s13131-013-0339-8

Citation:

SHAO Changwei, LIU Geng, LIU Shanshan, LIU Changlin, CHEN Songlin. Characterization of the cyp19a1a gene froma BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts[J]. Acta Oceanologica Sinica, 2013, 32(8): 35-43. doi: 10.1007/s13131-013-0339-8

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Characterization of the cyp19a1a gene froma BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts

doi: 10.1007/s13131-013-0339-8

1.
Ocean University of China, Qingdao 266071, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China
2.
BGI-Shenzhen, Shenzhen 518000, China
3.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China

Received Date: 2012-06-11
Rev Recd Date: 2012-12-22

Abstract

Abstract

The cyp19a1a gene encodes an aromatase that plays a key role in sex differentiation of the gonad. The first bacterial artificial chromosome (BAC) sequence of half-smooth tongue sole (Cynoglossus semilaevis) containing the intact cyp19a1a gene was reported and the conservation and synteny of the cyp19a1a gene among teleosts were analyzed in the study. The BAC is 107 367 bp in size, with an overall guanine-cytosine (GC) content of 43.44%, and contains 4.38% transposable elements. Nine genes were predicted, including seven functional genes and two hypothetical genes. The cyp19a1a gene of all tested teleosts has nine exons and eight introns, and potential binding sites flanking the transcriptional start site are conserved. The expression pattern among teleosts is also similar during ovarian differentiation. Synteny analysis revealed a conserved gene cluster PKH4B-SL9A5-FHOD3-CEBPG-CEBPA among teleosts. These findings suggest that, among teleosts, cyp19a1a genes not only have similar genomic structures, but also have conserved functions. The genomic environment of cyp19a1a in tongue sole is not universal in teleosts, reflecting the particular evolution of tongue sole cyp19a1a after it diverged from the other teleosts.
- BAC,
- cyp19a1a,
- conservation,
- gene synteny,
- half-smooth tongue sole

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References(59)

References

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