Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic <i>Vibrio harveyi</i>

QIN Yingxue; YAN Qingpi; SU Yongquan; LI Haiping; ZOU Wenzheng

doi:10.1007/s13131-013-0341-1

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Article Navigation > Acta Oceanologica Sinica > 2013 > 32(8): 55-60

QIN Yingxue, YAN Qingpi, SU Yongquan, LI Haiping, ZOU Wenzheng. Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi[J]. Acta Oceanologica Sinica, 2013, 32(8): 55-60. doi: 10.1007/s13131-013-0341-1

Citation:

QIN Yingxue, YAN Qingpi, SU Yongquan, LI Haiping, ZOU Wenzheng. Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi[J]. Acta Oceanologica Sinica, 2013, 32(8): 55-60. doi: 10.1007/s13131-013-0341-1

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Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi

doi: 10.1007/s13131-013-0341-1

1.
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China;Fisheries College, Jimei University, Xiamen 361021, China
2.
College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China

Received Date: 2012-04-09
Rev Recd Date: 2012-12-07

Abstract

Abstract

Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (GenBank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (GenBank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth,motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energymetabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotacticmotility of V. harveyi.
- chemotactic motility,
- Vibrio harveyi,
- transposon,
- virulence

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References(38)

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