Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (<i>Marsupenaeus japonicus</i>)

ZHANG Man; MAO Yong; WANG Jun; FENG Wenrong; SONG Xiaohong; SU Yongquan

doi:10.1007/s13131-015-0623-x

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Article Navigation > Acta Oceanologica Sinica > 2015 > 34(2): 74-83

ZHANG Man, MAO Yong, WANG Jun, FENG Wenrong, SONG Xiaohong, SU Yongquan. Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus)[J]. Acta Oceanologica Sinica, 2015, 34(2): 74-83. doi: 10.1007/s13131-015-0623-x

Citation:

ZHANG Man, MAO Yong, WANG Jun, FENG Wenrong, SONG Xiaohong, SU Yongquan. Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus)[J]. Acta Oceanologica Sinica, 2015, 34(2): 74-83. doi: 10.1007/s13131-015-0623-x

Citation:

ZHANG Man, MAO Yong, WANG Jun, FENG Wenrong, SONG Xiaohong, SU Yongquan. Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus)[J]. Acta Oceanologica Sinica, 2015, 34(2): 74-83. doi: 10.1007/s13131-015-0623-x

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Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus)

doi: 10.1007/s13131-015-0623-x

College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China

Received Date: 2013-10-05
Rev Recd Date: 2014-01-03

Abstract

Abstract

C-type lectins are among the most significant pattern recognition receptors (PRRs) found in invertebrate. They are a class of carbohydrate-binding proteins that can recognize specific sugar moieties on the surface of pathogens. In the present study, a novel C-type lecitn (termed MjLectin) from kuruma shrimp Marsupenaeus japonicus was identified. The full-length cDNA of MjLectin was 1 245 bp with a 1 011 bp open reading frame (ORF) that encoded a polypeptide of 336 amino acid residues. MjLectin consisted of two tandemly arrayed carbohydrate-recognition domains (CRDs), unlike other reported M. japonicus C-type lectins with only one CRD. It showed a high similarity to other shrimp dual-CRD lectins. Among the Ca²⁺-binding Site 2, the tripeptide motif dictating the carbohydrate binding specificity was exhibited as a rare mutant LPN (Leu₁₃₄-Pro₁₃₅-Asn₁₃₆) in CRD1 and a traditional EPN (Glu₂₉₉-Pro₃₀₀-Asn₃₀₁) in CRD2, respectively. MjLectin showed a specific expression pattern in both tissue and cellular levels, for its mRNA transcript was mainly expressed in the F-cells of the hepatopancreas. After white spot syndrome virus (WSSV) challenge (3.6×10⁸ virions/μL), the expression of MjLectin in the hepatopancreas was up-regulated significantly at 48 h (P<0.01) compared with the control group. These results suggested that MjLectin might be involved in the innate immune defense against WSSV infection.
- C-type lectin,
- Marsupenaeus japonicus,
- carbohydrate-recognition domain (CRD),
- gene expression,
- white spot syndrome virus (WSSV)

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References(39)

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