Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea

HE Hui; ZHEN Yu; MI Tiezhu; LU Xinglan; YU Zhigang

doi:10.1007/s13131-015-0710-z

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Article Navigation > Acta Oceanologica Sinica > 2015 > 34(8): 83-92

HE Hui, ZHEN Yu, MI Tiezhu, LU Xinglan, YU Zhigang. Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea[J]. Acta Oceanologica Sinica, 2015, 34(8): 83-92. doi: 10.1007/s13131-015-0710-z

Citation:

HE Hui, ZHEN Yu, MI Tiezhu, LU Xinglan, YU Zhigang. Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea[J]. Acta Oceanologica Sinica, 2015, 34(8): 83-92. doi: 10.1007/s13131-015-0710-z

Citation:

HE Hui, ZHEN Yu, MI Tiezhu, LU Xinglan, YU Zhigang. Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea[J]. Acta Oceanologica Sinica, 2015, 34(8): 83-92. doi: 10.1007/s13131-015-0710-z

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Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea

doi: 10.1007/s13131-015-0710-z

1.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China
2.
Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China;College of Environmental Science and Engineering, Qingdao 266100, China
3.
College of Environmental Science and Engineering, Qingdao 266100, China
4.
Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao 266100, China

Received Date: 2014-05-13
Rev Recd Date: 2014-10-22

Abstract

Abstract

Ammonia oxidation plays a significant role in the nitrogen cycle in marine sediments. Seasonal and spatial distribution of ammonia-oxidizing archaea (AOA) and betaproteobacteria (β-AOB) in surface sediments from the East China Sea (ECS) were investigated using ammonia monooxygenase α subunit (amoA) gene. In order to characterize the community of AOA and β-AOB, real-time quantitative polymerase chain reaction (qPCR) was carried out in this study, along with environmental parameters. The abundance of β-AOB amoA gene (2.17×10⁶-4.54×10⁷ copy numbers per gram wet weight sediment) was always greater than that of AOA amoA gene (2.18×10⁵-9.89×10⁶ copy numbers per gram wet weight sediment) in all sampling stations. The qPCR results were correlated with environmental parameters. AOA amoA gene copy numbers in April were positively related to temperature and nitrite concentration (p <0.05). β-AOB amoA gene copy numbers in August correlated negatively with salinity (p <0.01), and correlated positively with ammonium concentration (p <0.05). With the increase of salinity, the amoA gene copy ratio of AOB to AOA had a tendency to decrease, which suggested β-AOB dominated in the area of high level ammonium and AOA preferred high salinity area.
- ammonia-oxidizing microorganisms,
- sediment,
- East China Sea

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