Molecular characteristics of three thymosin-repeat proteins from <i>Marsupenaeus japonicus</i> and their responses to WSSV infection

MA Jinyou; RUAN Lingwei; XU Xun; GAO Zhaoming

doi:10.1007/s13131-016-0835-8

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Article Navigation > Acta Oceanologica Sinica > 2016 > 35(4): 44-50

MA Jinyou, RUAN Lingwei, XU Xun, GAO Zhaoming. Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection[J]. Acta Oceanologica Sinica, 2016, 35(4): 44-50. doi: 10.1007/s13131-016-0835-8

Citation:

MA Jinyou, RUAN Lingwei, XU Xun, GAO Zhaoming. Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection[J]. Acta Oceanologica Sinica, 2016, 35(4): 44-50. doi: 10.1007/s13131-016-0835-8

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Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection

doi: 10.1007/s13131-016-0835-8

1.
College of Animal Sciences, Henan Institute of Science and Technology, Xinxiang 453003, China;State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, China;Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
2.
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, China;Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received Date: 2014-10-23
Rev Recd Date: 2015-09-24

Abstract

Abstract

β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis, antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags(EST) from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 cDNAs possessed open reading frames that encoded 166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4, mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps. The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps.
- β-thymosin,
- thymosin-repeat proteins,
- white spot syndrome virus(WSSV),
- Marsupenaeus japonicus

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References(32)

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