Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in <i>Crassostrea ariakensis</i>

WANG Hui; YANG Hongshuai; LIU Jiahui; LI Yanhong; LIU Zhigang

doi:10.1007/s13131-016-0838-5

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Article Navigation > Acta Oceanologica Sinica > 2016 > 35(4): 51-57

WANG Hui, YANG Hongshuai, LIU Jiahui, LI Yanhong, LIU Zhigang. Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis[J]. Acta Oceanologica Sinica, 2016, 35(4): 51-57. doi: 10.1007/s13131-016-0838-5

Citation:

WANG Hui, YANG Hongshuai, LIU Jiahui, LI Yanhong, LIU Zhigang. Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis[J]. Acta Oceanologica Sinica, 2016, 35(4): 51-57. doi: 10.1007/s13131-016-0838-5

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Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis

doi: 10.1007/s13131-016-0838-5

1.
School of Life Sciences, Huaiyin Normal University, Huaian 223300, China
2.
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China

Received Date: 2014-11-30
Rev Recd Date: 2015-09-14

Abstract

Abstract

Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD(SOD1) and Mn-SOD(SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature(18-34℃) and copper ion concentration(0.1-1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant(P<0.01), the quadratic effect of temperature was significant(P<0.05); the linear effect of copper ion concentration was not significant(P>0.05), while the quadratic effect of copper ion concentration was highly significant(P<0.01); the interactive effect of temperature and copper ion concentration was not significant(P>0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R² and predictive R² as high as 0.9616 and 0.8207, respectively, suggesting that the goodness-of-fit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.
- Crassostrea ariakensis,
- superoxide dismutase,
- temperature,
- copper ion concentration,
- combined effect

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