Flow cytometric analysis of DNA content for four commercially important crabs in China

LIU Lei; CUI Zhaoxia; SONG Chengwen; LIU Yuan; HUI Min; WANG Chunlin

doi:10.1007/s13131-016-0876-z

Article Contents

Article Navigation > Acta Oceanologica Sinica > 2016 > 35(6): 7-11

LIU Lei, CUI Zhaoxia, SONG Chengwen, LIU Yuan, HUI Min, WANG Chunlin. Flow cytometric analysis of DNA content for four commercially important crabs in China[J]. Acta Oceanologica Sinica, 2016, 35(6): 7-11. doi: 10.1007/s13131-016-0876-z

Citation:

LIU Lei, CUI Zhaoxia, SONG Chengwen, LIU Yuan, HUI Min, WANG Chunlin. Flow cytometric analysis of DNA content for four commercially important crabs in China[J]. Acta Oceanologica Sinica, 2016, 35(6): 7-11. doi: 10.1007/s13131-016-0876-z

Citation:

PDF( 12126 KB)

Flow cytometric analysis of DNA content for four commercially important crabs in China

doi: 10.1007/s13131-016-0876-z

1.
School of Marine Sciences, Ningbo University, Ningbo 315211, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2015-04-02
Rev Recd Date: 2015-12-16

Abstract

Abstract

The genome size (C-value) of an organism is referring to the DNA content of its non-replicated haploid chromosome complement, generally deduced from measuring somatic diploid nuclei. We presented genome size (C-value) data obtained by flow cytometry for four commercially important crabs (Portunus trituberculatus, Charybdis japonica, Scylla paramamosain, and Eriocheir sinensis) common in the coast of China. Gallus domesticus (2C=2.5 pg) was used as the internal standard. The results showed that the C-value for P. trituberculatus, C. japonica, S. paramamosain, and E. sinensis were (2.31±0.01) pg, (2.33±0.03) pg, (1.64±0.02) pg, and (2.29±0.03) pg, respectively. The C-value of P. trituberculatus, C. japonica and S. paramamosain were reported for the first time. The data represented by the four species indicated that they had lower DNA contents than average DNA values in crustaceans ((4.99±0.48) pg), and three of the four values were very similar if not identical. The results provide useful data for future studies in the fields of biodiversity, species conservation, and phylogeny of these commercial crabs. They will also be helpful in instructing the hybridization breeding program and estimating the cost of the whole genome sequencing project.
- genome size,
- flow cytometry,
- crabs,
- Portunidae,
- Grapsidae

FullText(HTML)

References(39)

References

Allen Jr S K. 1983. Flow cytometry: assaying experimental polyploid fish and shellfish. Aquaculture, 33(1-4): 317-328

Bachère E, Chagot D, Grizel H. 1988. Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation. Developmental & Comparative Immun-ology, 12(3): 549-559

Bachmann K, Rheinsmith E L. 1973. Nuclear DNA amounts in pacific Crustacea. Chromosoma, 43(3): 225-236

Beaulieu J M, Leitch I J, Knight C A. 2007. Genome size evolution in relation to leaf strategy and metabolic rates revisited. Annals of Botany, 99(3): 495-505

Bennett M D, Bhandol P, Leitch I J. 2000. Nuclear DNA amounts in angiosperms and their modern uses-807 new estimates. An-nals of Botany, 86(4): 859-909

Birstein V J, Poletaev A I, Goncharov B F. 1993. DNA content in euras-ian sturgeon species determined by Flow Cytometry. Cyto-metry, 14(4): 377-383

Bonnivard E, Catrice O, Ravaux J, et al. 2009. Survey of genome size in 28 hydrothermal vent species covering 10 families. Genome, 52(6): 524-536

Chen Songlin, Zhang Guojie, Shao Changwei, et al. 2014. Whole-gen-ome sequence of a flatfish provides insights into ZW sex chro-mosome evolution and adaptation to a benthic lifestyle. Nature Genetics, 46(3): 253-260

Crissman H A, Stevenson A P, Kissane R J, et al. 1979. Techniques for quantitative staining of cellular DNA for flow cytometry analys-is. In: Melamed M R, Mullaney P F, Mendelsohn M L, eds. Flow Cytometry and Sorting. New York: John Wiley & Sons, 243-261

Dillon Jr R T. 1989. Karyotypic evolution in Pleurocerid snails: I. Gen-omic DNA estimated by flow cytometry. Malacologia, 31(1): 197-203

Dole.el J, Barto. J. 2005. Plant DNA flow cytometry and estimation of nuclear genome size. Annals of Botany, 95(1): 99-110

Dole.el J, Barto. J, Voglmayr H, et al. 2003. Nuclear DNA content and genome size of trout and human. Cytometry Part A. The Journ-al of the International Society for Analytical Cytology, 51(2): 127-128

Fafan.el M, Bihari N, Smodlaka M, et al. 2008. Hemocytes/coelomo-cytes DNA content in five marine invertebrates: cell cycles and genome sizes. Biologia, 63(5): 730-736

Fishery Bureau, Ministry of Agriculture, China. 2014. China Fisheries Yearbook 2014 (in Chinese). Beijing: China Agriculture Press

Grau S M, Cooke I M. 1992. Peptidergic neurons of the crab, Card-isoma carnifex, in defined culture maintain characteristic mor-phologies under a variety of conditions. Cell Tissue Research, 270(2): 303-317

Gregory T R. 2001a. Coincidence, coevolution, or causation? DNA content, cell size, and the C-value enigma. Biological Reviews, 76(1): 65-101

Gregory T R. 2001b. The bigger the C-Value, the larger the cell: gen-ome size and red blood cell size in vertebrates. Blood Cells, Mo-lecules, and Diseases, 27(5): 830-843

Gregory T R. 2002. A bird's-eye view of the C-value enigma: genome size, cell size, and metabolic rate in the class aves. Evolution, 56(1): 121-130

Gregory T R. 2016. Animal genome size database. http://www.gen-omesize.com

Griffith O L, Moodie G E E, Civetta A. 2003. Genome size and longev-ity in fish. Experimental Gerontology, 38(3): 333-337

Hameed A S S, Yoganandhan K, Sathish S, et al. 2001. White spot syn-drome virus (WSSV) in two species of freshwater crabs (Paratel-phusa hydrodomous and P. pulvinata). Aquaculture, 201(3-4): 179-186

Jeffery N W. 2012. The first genome size estimates for six species of krill (Malacostraca, Euphausiidae): large genomes at the north and south poles. Polar Biology, 35(6): 959-962

Marie D, Brown S C. 1993. A cytometric exercise in plant DNA histo-grams, with 2C values for 70 species. Biology of the Cell, 78(1-2): 41-51

Musich P R, Skinner D M. 1972. A cytological study of the DNA of the Bermuda land crab, Gecarcinus lateralis. Journal of Cell Bio-logy, 55: 184a

Petrov D A. 2001. Evolution of genome size: new approaches to an old problem. Trends in Genetics, 17(1): 23-28

Prosperi E, Giangarè M C, Bottiroli G. 1991. Nuclease-induced DNA structural changes assessed by flow cytometry with the intercal-ating dye propidium iodide. Cytometry, 12(4): 323-329

Rasch E M, Wyngaard G A. 2006. Genome sizes of cyclopoid cope-pods (Crustacea): evidence of evolutionary constraint. Biolo-gical Journal of the Linnean Society, 87(4): 625-635

Rayburn A L, Auger J A, McMurphy L M. 1992. Estimating percentage constitutive heterochromatin by flow cytometry. Experimental Cell Research, 198(1): 175-178

Rees D J, Dufresne F, Glémet H, et al. 2007. Amphipod genome sizes: first estimates for Arctic species reveal genomic giants. Gen-ome, 50(2): 151-158

Rheinsmith E L, Hinegardner R, Bachmann K. 1974. Nuclear DNA amounts in Crustacea. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 48(3): 343-348

Shapiro H M. 1988. Practical Flow Cytometry. 2nd ed. New York: Alan R Liss Inc, 353

Shapiro H M. 2003. Practical Flow Cytometry. 4th ed. New York: Wiley-Liss

Smith E M, Gregory T R. 2009. Patterns of genome size diversity in the ray-finned fishes. Hydrobiologia, 625(1): 1-25

Tiersch T R, Chandler R W, Wachtel S S, et al. 1989. Reference stand-ards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry, 10(6): 706-710

Vinogradov A E. 2004. Genome size and extinction risk in vertebrates. Proceedings of the Royal Society B: Biological Sciences, 271(1549): 1701-1705

Xia Xuhua. 1995. Body temperature, rate of biosynthesis, and evolu-tion of genome size. Molecular Biology and Evolution, 12(5): 834-842

Yi S, Streelman J T. 2005. Genome size is negatively correlated with effective population size in ray-finned fish. Trends in Genetics, 21(12): 643-646

Zhang Guofan, Fang Xiaodong, Guo Ximing, et al. 2012. The oyster genome reveals stress adaptation and complexity of shell form-ation. Nature, 490(7418): 49-54

Zhu Zeyuan, Yang Jie, Shi Yonghui, et al. 2007. Determination of gen-ome size of Eriocheir sinensis with SYBR Green I real-time fluor-escence quantitative PCR. Jiangsu Agricultural Sciences (in Chinese), (5): 164-166

Relative Articles

Supplements(0)

Cited By

Proportional views