Diversity of the aerobic anoxygenic phototrophy gene <i>pufM</i> in Arctic and Antarctic coastal seawaters

ZENG Yinxin; DONG Peiyan; QIAO Zongyun; ZHENG Tianling

doi:10.1007/s13131-016-0877-y

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Article Navigation > Acta Oceanologica Sinica > 2016 > 35(6): 68-77

ZENG Yinxin, DONG Peiyan, QIAO Zongyun, ZHENG Tianling. Diversity of the aerobic anoxygenic phototrophy gene pufM in Arctic and Antarctic coastal seawaters[J]. Acta Oceanologica Sinica, 2016, 35(6): 68-77. doi: 10.1007/s13131-016-0877-y

Citation:

ZENG Yinxin, DONG Peiyan, QIAO Zongyun, ZHENG Tianling. Diversity of the aerobic anoxygenic phototrophy gene pufM in Arctic and Antarctic coastal seawaters[J]. Acta Oceanologica Sinica, 2016, 35(6): 68-77. doi: 10.1007/s13131-016-0877-y

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Diversity of the aerobic anoxygenic phototrophy gene pufM in Arctic and Antarctic coastal seawaters

doi: 10.1007/s13131-016-0877-y

1.
Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, China;College of Biological Engineering, Jimei University, Xiamen 361021, China
2.
State Key Laboratory of Marine Environmental Science, School of Life Sciences, Xiamen University, Xiamen 361005, China

Received Date: 2015-04-14
Rev Recd Date: 2015-08-07

Abstract

Abstract

Aerobic anoxygenic phototrophic (AAP) bacteria serve important functions in marine carbon and energy cycling because of their capability to utilize dissolved organic substrates and harvest light energy. AAP bacteria are widely distributed in marine environments, and their diversity has been examined in marine habitats. However, information about AAP bacteria at high latitudes remains insufficient to date. Therefore, this study determined the summer AAP bacterial diversity in Arctic Kongsfjorden and in the Antarctic coastal seawater of King George Island on the basis of pufM, a gene that encodes a pigment-binding protein subunit of the reaction center complex. Four pufM clone libraries were constructed, and 674 positive clones were obtained from four investigated stations (two in Kongsfjorden and two in the Antarctic Maxwell Bay). Arctic clones were clustered within the Alphaproteobacteria, whereas Antarctic clones were classified into the Alphaproteobacteria and Betaproteobacteria classes. Rhodobacteraceae-like pufM genes dominated in all samples. In addition, sequences closely related to pufM encoded on a plasmid in Sulfitobacter guttiformis were predominant in both Arctic and Antarctic samples. This result indicates the transpolar or even global distribution of pufM genes in marine environments. Meanwhile, differences between the Arctic and Antarctic sequences may prove polar endemism. These results indicate the important role of Rhodobacteraceae as AAP bacteria in bipolar coastal waters.
- diversity,
- aerobic anoxygenic phototrophic bacteria,
- pufM,
- Arctic,
- Antarctic

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References(46)

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