Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China

MA Yuexin; TAO Wei; LIU Changfa; LIU Jiao; YANG Zhiping; LI Jin; LIU Jichen

doi:10.1007/s13131-017-1048-5

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Article Navigation > Acta Oceanologica Sinica > 2017 > 36(6): 80-88

MA Yuexin, TAO Wei, LIU Changfa, LIU Jiao, YANG Zhiping, LI Jin, LIU Jichen. Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China[J]. Acta Oceanologica Sinica, 2017, 36(6): 80-88. doi: 10.1007/s13131-017-1048-5

Citation:

MA Yuexin, TAO Wei, LIU Changfa, LIU Jiao, YANG Zhiping, LI Jin, LIU Jichen. Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China[J]. Acta Oceanologica Sinica, 2017, 36(6): 80-88. doi: 10.1007/s13131-017-1048-5

Citation:

MA Yuexin, TAO Wei, LIU Changfa, LIU Jiao, YANG Zhiping, LI Jin, LIU Jichen. Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China[J]. Acta Oceanologica Sinica, 2017, 36(6): 80-88. doi: 10.1007/s13131-017-1048-5

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Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China

doi: 10.1007/s13131-017-1048-5

1.
Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China
2.
Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China
3.
Dalian Huixin Titanium Equipment Development Company Limited, Dalian 116039, China
4.
Institute of Ocean and Fisheries of Panjin, Panjin 124001, China

Received Date: 2016-01-16
Rev Recd Date: 2016-08-02

Abstract

Abstract

The effects of nitrogen (N) addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-time quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis (DGGE) in a salt marsh located in Shuangtai Estuary, China. The sediment samples were collected from plots treated with different amounts of a single N fertilizer (urea supplied at 0.1, 0.2, 0.4 and 0.8 g/kg (nitrogen content in sediment) and different forms of N fertilizers (urea, (NH₄)₂SO₄, and NH₄NO₃, each supplied at 0.2 g/kg (calculated by nitrogen). The fertilizers were applied 1-4 times during the plant-growing season in May, July, August, and September of 2013. Untreated plots were included as a control. The results showed that both the amount and form of N positively influenced microbial biomass carbon, microbial biomass nitrogen, and bacterial abundance. The DGGE profiles revealed that the bacterial community composition was also affected by the amount and form of N. Thus, our findings indicate that short-term N amendment increases microbial biomass and bacterial abundance, and alters the structure of bacterial community.
- fertilization,
- microbial biomass,
- 16S rRNA gene abundance,
- bacterial community,
- salt marsh

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References(39)

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