Pathogenic bacterium <i>Vibrio harveyi</i>:an endosymbiont in the marine parasitic ciliate protozoan <i>Cryptocaryon irritans</i>

QIAO Ying; WANG Jun; MAO Yong; LIU Min; CHEN Ruanni; SU Yongquan; KE Qiaozhen; HAN Kunhuang; ZHENG Weiqiang

doi:10.1007/s13131-017-1050-y

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Article Navigation > Acta Oceanologica Sinica > 2017 > 36(10): 115-119

QIAO Ying, WANG Jun, MAO Yong, LIU Min, CHEN Ruanni, SU Yongquan, KE Qiaozhen, HAN Kunhuang, ZHENG Weiqiang. Pathogenic bacterium Vibrio harveyi:an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans[J]. Acta Oceanologica Sinica, 2017, 36(10): 115-119. doi: 10.1007/s13131-017-1050-y

Citation:

QIAO Ying, WANG Jun, MAO Yong, LIU Min, CHEN Ruanni, SU Yongquan, KE Qiaozhen, HAN Kunhuang, ZHENG Weiqiang. Pathogenic bacterium Vibrio harveyi:an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans[J]. Acta Oceanologica Sinica, 2017, 36(10): 115-119. doi: 10.1007/s13131-017-1050-y

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Pathogenic bacterium Vibrio harveyi:an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans

doi: 10.1007/s13131-017-1050-y

1.
State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
2.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
3.
State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China

Received Date: 2016-04-29

Abstract

Abstract

Vibrio harveyi, known as a pathogenic bacterium caused severe secondary bacterial infections of the large yellow croaker Larimichthys crocea, was identified as an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans. Meta 16S sequencing method was used to identify the bacterial flora in C. irritans, and V. harveyi was isolated via culture-dependent method. Vibrio harveyi was observed in cytoplasm of C. irritans at the stage of tomont both by transmission electron microscopy and by Fluorescence in situ hybridization; no signal, however, was detected in nucleus area. The relationship between V. harveyi and C. irritans and the role of endosymbiotic V. harveyi in C. irritans merit further investigation.
- endosymbiotic bacterium,
- protozoan,
- Vibrio harveyi,
- Cryptocaryon irritans,
- Meta16S sequencing

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References(19)

References

Benediktsdóttir, Helgason, Sigurjónsdóttir. 1998. Vibrio spp. isolated from salmonids with shallow skin lesions and reared at low temperature. J Fish Dis, 21(1):19-28

Burgess P J, Matthews R A. 1994. Cryptocaryon irritans (Ciliophora):photoperiod and transmission in marine fish. J Mar Biol Assoc U K, 74:535-542

Colorni A. 1987. Biology of Cryptocaryon irritans and strategies for its control. Aquaculture, 67(1-2):236-237

Colorni A, Diamant A. 1993. Ultrastructural features of Cryptocaryon irritans, a ciliate parasite of marine fish. Eur J Protistol, 29(4):425-434

Dan X M, Li A X, Lin X T, et al. 2006. A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus. Aquaculture, 258(1-4):127-133

Diamant A, Issar G, Colorni A, et al. 1991. A pathogenic Cryptocaryon-like ciliate from the Mediterranean Sea. Bull Eur Assoc Fish Pathol, 11(3):122-124

Diggles B K. 1997. Some information on the morphology of Cryptocaryon irritans from South-East Queensland, Australia. Eur J Protistol, 33(2):200-210

Fokin S I. 2004. Bacterial endocytobionts of ciliophora and their interactions with the host Cell. Int Rev Cytol, 236:181-249

Liu Zhenyong, Lin Xiaojin, Xie Youquan, et al. 2012. Research on death caused by secondary bacterial infection of Cryptocaryon irritans on Pseudosciaena crocea. J Fujian Fish (in Chinese), 34(1):11-15

Luo X C, Xie M Q, Zhu X Q, et al. 2008. Some characteristics of host-parasite relationship for Cryptocaryon irritans isolated from South China. Parasitol Res, 102(6):1269-1275

Manz W, Amann R, Ludwig W, et al. 1992. phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria:problems and solutions. Syst Appl Microbiol, 15(4):593-600

Preer J R Jr, Preer L B, Jurand A, et al. 1974. Kappa and other endosymbionts in Paramecium aurelia. Bacteriol Rev, 38(2):113-163

Saeed A, Abd H, Edvinsson B, et al. 2007. Vibrio cholerae-Acanthamoeba castellanii interaction showing endosymbiont-host relation. Symbiosis, 44(1-3):153-158

Schloss P D, Westcott S L, Ryabin T, et al. 2009. Introducing mothur:open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol, 75(23):7537-7541

Schweikert M, Fujishima M, Görtz H D. 2013. Symbiotic Associations Between Ciliates and Prokaryotes. Berlin Heidelberg:Springer, 427-463

Sun H Y, Zhu X Q, Xie M Q, et al. 2006. Characterization of Cryptocaryon irritans isolates from marine fishes in Mainland China by ITS ribosomal DNA sequences. Parasitol Res, 99(2):160-166

Verni F, Gualtieri P. 1997. Feeding behaviour in ciliated protists. Micron, 28(6):487-504

Yoon J H, Lee S T, Shin Y K, et al. 1996. Rapid identification of Saccharomonospora strains by multiplex PCR using species-specific primers within the 16S rRNA gene. J Microbiol Methods, 27(1):89-95

Yu Yinchun, Han Yanmei, Wang Xinyi, et al. 2015. Identification of pathogenic from Carassius auratus triploid with skin bleeding and ulcers and its prevention of drug screening. J Agric Catastrophol, 5(3):28-30, 62

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