Differential gene expression in the body wall of the sea cucumber (Apostichopus japonicus) under strong lighting and dark conditions

ZHANG Libin; FENG Qiming; SUN Lina; FANG Yan; XU Dongxue; ZHANG Tao; YANG Hongsheng

doi:10.1007/s13131-017-1101-4

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Article Navigation > Acta Oceanologica Sinica > 2018 > 37(5): 54-66

ZHANG Libin, FENG Qiming, SUN Lina, FANG Yan, XU Dongxue, ZHANG Tao, YANG Hongsheng. Differential gene expression in the body wall of the sea cucumber (Apostichopus japonicus) under strong lighting and dark conditions[J]. Acta Oceanologica Sinica, 2018, 37(5): 54-66. doi: 10.1007/s13131-017-1101-4

Citation:

ZHANG Libin, FENG Qiming, SUN Lina, FANG Yan, XU Dongxue, ZHANG Tao, YANG Hongsheng. Differential gene expression in the body wall of the sea cucumber (Apostichopus japonicus) under strong lighting and dark conditions[J]. Acta Oceanologica Sinica, 2018, 37(5): 54-66. doi: 10.1007/s13131-017-1101-4

Citation:

ZHANG Libin, FENG Qiming, SUN Lina, FANG Yan, XU Dongxue, ZHANG Tao, YANG Hongsheng. Differential gene expression in the body wall of the sea cucumber (Apostichopus japonicus) under strong lighting and dark conditions[J]. Acta Oceanologica Sinica, 2018, 37(5): 54-66. doi: 10.1007/s13131-017-1101-4

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Differential gene expression in the body wall of the sea cucumber (Apostichopus japonicus) under strong lighting and dark conditions

doi: 10.1007/s13131-017-1101-4

1.
CAS Key laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
2.
CAS Key laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China
3.
School of Agriculture, Ludong University, Yantai 264025, China
4.
College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China

Received Date: 2017-01-25
Rev Recd Date: 2017-03-09

Abstract

Abstract

Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light ("light"), normal light ("control") and fully dark ("dark") environment. In the comparisons of "control" vs. "dark", "control" vs. "light" and "dark" vs. "light", 1 161, 113 and 1 705 differentially expressed genes (DEGs) were identified following the criteria of|log₂ ratio| ≥ 1 and FDR ≤ 0.001, respectively. Gene ontology analysis showed that "cellular process" and "binding" enriched the most DEGs in the category of "biological process" and "molecular function", while "cell" and "cell part" enriched the most DEGs in the category of "cellular component". And the DEGs were mapped to 214, 41 and 229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light.
- sea cucumber,
- Apostichopus japonicus,
- gene expression,
- dark,
- light,
- body wall

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References(26)

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Studies on the proteins of 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