<i>Heterocapsa bohaiensis</i> sp. nov. (Peridiniales: Dinophyceae): a novel marine dinoflagellate from the Liaodong Bay of Bohai Sea, China

XIAO Jie; SUN Na; ZHANG Yiwen; SUN Ping; LI Yan; PANG Min; LI Ruixiang

doi:10.1007/s13131-018-1296-z

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Article Navigation > Acta Oceanologica Sinica > 2018 > 37(10): 18-27

XIAO Jie, SUN Na, ZHANG Yiwen, SUN Ping, LI Yan, PANG Min, LI Ruixiang. Heterocapsa bohaiensis sp. nov. (Peridiniales: Dinophyceae): a novel marine dinoflagellate from the Liaodong Bay of Bohai Sea, China[J]. Acta Oceanologica Sinica, 2018, 37(10): 18-27. doi: 10.1007/s13131-018-1296-z

Citation:

XIAO Jie, SUN Na, ZHANG Yiwen, SUN Ping, LI Yan, PANG Min, LI Ruixiang. Heterocapsa bohaiensis sp. nov. (Peridiniales: Dinophyceae): a novel marine dinoflagellate from the Liaodong Bay of Bohai Sea, China[J]. Acta Oceanologica Sinica, 2018, 37(10): 18-27. doi: 10.1007/s13131-018-1296-z

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Heterocapsa bohaiensis sp. nov. (Peridiniales: Dinophyceae): a novel marine dinoflagellate from the Liaodong Bay of Bohai Sea, China

doi: 10.1007/s13131-018-1296-z

1.
Key Laboratory of Science and Engineering for Marine Ecology and Environment, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
2.
Guanghe Crab Industry Limited Company, Panjin 124200, China
3.
School of Food and Environment, Dalian University of Technology, Panjin 124221, China
4.
Key Laboratory of Science and Engineering for Marine Ecology and Environment, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2017-08-01

Abstract

Abstract

A small armed dinoflagellate bloomed in the aquaculture ponds off the coast of Liaodong Bay, Bohai Sea of China, resulting in heavy mortalities of the cultured prawns (Penaeus japonicus) and larvae of Chinese mitten handed crabs (Eriocheir sinensis). The bloom-forming species was successfully isolated, and cellular morphology of the specimen was consequently investigated through light, fluorescent and electron microscopy. The small ((14.4±1.6) μm in length) ellipsoid cells show typical Heterocapsa thecal plate arrangement (Po, cp, 5', 3a, 7'', 6c, 5s, 5''', 2''''). The episome is evidently bigger than the hyposome. One to three spherical pyrenoids are located above or beside the large elongated nucleus. The body scale is characterized by a triangle basal plate with one central upright and nine peripheral spines. Above all, Heterocapsa bohaiensis could be distinguished from other Heterocapsa species by the combination of the cell size, morphology, cellular structure and body scale. Sequence analyses of both ITS and LSU regions reveal the significant genetic divergence between H. bohaiensis and other established species in this genus, further supporting novelty of this species. Noticeably, different sample treatment methods resulted in morphological variation of the apical pore complex (APC) of H. bohaiensis, which needs to be taken into account in future study.
- Heterocapsa,
- harmful algal bloom,
- thecal plate,
- body scale,
- ITS

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References(53)

References

Attaran-Fariman G, Javid P. 2013. The phylogeny of Heterocapsa sp. (Dinophyceae) isolated from the south coast of Iran during a Cochlodinium polykrikoides bloom. Turkish Journal of Botany, 37(4): 778–783

Basti L, Endo M, Segawa S, et al. 2015. Prevalence and intensity of pathologies induced by the toxic dinoflagellate, Heterocapsa circularisquama, in the Mediterranean mussel, Mytilus galloprovincialis. Aquatic Toxicology, 163: 37–50

Basti L, Nagai S, Watanabe S, et al. 2016. Neuroenzymatic activity and physiological energetics in Manila clam, Ruditapes philippinarum, during short-term sublethal exposure to harmful alga, Heterocapsa circularisquama. Aquatic Toxicology, 176: 76–87

D’Onofrio G, Marino D, Bianco L, et al. 1999. Toward an assessment on the taxonomy of dinoflagellates that produce calcareous cysts (Calciodinelloideae, Dinophyceae): a morphological and molecular approach. Journal of Phycology, 35(5): 1063–1078

Dodge J D, Hermes H B. 1981. A scanning electron microscopical study of the apical pores of marine dinoflagellates (Dinophyceae). Phycologia, 20(4): 424–430

Du Jiayin. 2005. The red tide organism Heterocapsa circularisquama and its effects on fisheries. Hebei Fisheries (in Chinese), (2): 15–16

Edgar R C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32(5): 1792–1797

Edvardsen B, Shalchian-Tabrizi K, Jakobsen K S, et al. 2003. Genetic variability and molecular phylogeny of Dinophysis species (Dinophyceae) from Norwegian waters inferred from single cell analyses of rDNA. Journal of Phycology, 39(2): 395–408

Fritz L, Triemer R E. 1985. A rapid simple technique utilizing Calcofluor White M2R for the visualization of dinoflagellate thecal plates. Journal of Phycology, 21(4): 662–664

Gómez F. 2005. A list of free-living dinoflagellate species in the world’s oceans. Acta Botanica Croatica, 64(1): 129–212

Gómez F. 2012. A checklist and classification of living dinoflagellates (Dinoflagellata, Alveolata). CICIMAR Oceánides, 27(1): 65–140

Gottschling M, Keupp H, Plötner J, et al. 2005. Phylogeny of calcareous dinoflagellates as inferred from ITS and ribosomal sequence data. Molecular Phylogenetics and Evolution, 36(3): 444–455

Hansen G. 1995. Analysis of the thecal plate pattern in the dinoflagellate Heterocapsa rotundata (Lohmann) comb. nov. (=Katodinium rotundatum (Lohmann) Loeblich). Phycologia, 34(2): 166–170

Herman E M, Sweeney B M. 1976. Cachonina illdefina sp. nov. (Dinophyceae): Chloroplast tubules and degeneration of the pyrenoid. Journal of Phycology, 12(2): 198–205

Hoppenrath M. 2017. Dinoflagellate taxonomy–a review and proposal of a revised classification. Marine Biodiversity, 47(2): 381–403

Horiguchi T. 1995. Heterocapsa circularisquama sp. nov. (Peridiniales, Dinophyceae): a new marine dinoflagellate causing mass mortality of bivalves in Japan. Phycological Research, 43(3): 129–136

Horiguchi T. 1997. Heterocapsa arctica sp. nov. (Peridiniales, Dinophyceae), a new marine dinoflagellate from the arctic. Phycologia, 36(6): 488–491

Iwataki M. 2008. Taxonomy and identification of the armored dinoflagellate genus Heterocapsa (Peridiniales, Dinophyceae). Plankton & Benthos Research, 3(3): 135–142

Iwataki M, Botes L, Sawaguchi T, et al. 2003. Cellular and body scale structure of Heterocapsa ovata sp. nov. and Heterocapsa orientalis sp. nov. (Peridiniales, Dinophyceae). Phycologia, 42(6): 629–637

Iwataki M, Hansen G, Sawaguchi T, et al. 2004. Investigations of body scales in twelve Heterocapsa species (Peridiniales, Dinophyceae), including a new species H. pseudotriquetra sp. nov. Phycologia, 43(4): 394–403

Iwataki M, Kawami H, Van Nguyen N, et al. 2009. Cellular and body scale morphology of Heterocapsa huensis sp. nov. (Peridiniales, Dinophyceae) found in Hue, Vietnam. Phycological Research, 57(2): 87–93

Iwataki M, Takayama H, Matsuoka K, et al. 2002a. Heterocapsa lanceolata sp. nov. and Heterocapsa horiguchii sp. nov. (Peridiniales, Dinophyceae), two new marine dinoflagellates from coastal Japan. Phycologia, 41(5): 470–479

Iwataki M, Wong M W, Fukuyo Y. 2002b. New record of Heterocapsa circularisquama (Dinophyceae) from Hong Kong. Fisheries Science, 68(5): 1161–1163

Kim H G. 1997. Recent harmful algal blooms and mitigation strategies in Korea. Ocean and Polar Research, 19(2): 185–192

Kononen K, Huttunen M, Kanoshina I, et al. 1999. Spatial and temporal variability of a dinoflagellate-cyanobacterium community under a complex hydrodynamical influence: a case study at the entrance to the Gulf of Finland. Marine Ecology Progress Series, 186: 43–57

Lindholm T, Nummelin C. 1999. Red tide of the dinoflagellate Heterocapsa triquetra (Dinophyta) in a ferry-mixed coastal inlet. Hydrobiologia, 393: 245–251

Liu Ruiyu. 2008. Checklist of Biota of Chinese Seas (in Chinese). Beijing: Science Press, 1–1267

Liu Xiaoxu. 2016. The preliminary study on growth and toxicity of Heterocapsa sp. and the effect of rearing Calanus sinicus (in Chinese) [dissertation]. Dalian: Dalian Ocean University

Liu Yan, Yan Aiju, Wu Huixian. 2009. The morphology of the Heterocapsa circularisquama and Chaetoceros gracilis (in Chinese). http://d.oldg.wanfangdata.com.cn/Conference_7259389.aspx [2010-09-29/2017-10-26]

Liu Shuang, Zhang Jimin, Zhang Hongliang, et al. 2014. Spatial-temporal distribution and hazard grading of red tide and variation of red-tide species in Qingdao adjacent sea area. Journal of Hydroecology (in Chinese), 35(4): 43–47

Loeblich Ⅲ A R. 1968. A new marine dinoflagellate genus Cachonina in axenic culture from the Salton Sea California USA with remarks on the genus Peridinium. Proceedings of The Biological Society of Washington, 81: 91–96

Loeblich Ⅲ A R, Schmidt R J, Sherley J L. 1981. Scanning electron microscopy of Heterocapsa pygmaea sp. nov., and evidence for polyploidy as a speciation mechanism in dinoflagellates. Journal of Plankton Research, 3(1): 67–79

Matsuyama Y, Nagai K, Mizuguchi T, et al. 1995. Ecological features and mass mortality of pearl oysters during red tides of Heterocapsa sp. in Ago Bay in 1992. Nippon Suisan Gakkaishi, 61(1): 35–41

Matsuyama Y, Uchida T, Honjo T. 1997. Toxic effects of the dinoflagellate Heterocapsa circularisquama on clearance rate of the blue mussel Mytilus galloprovincialis. Marine Ecology Progress Series, 146: 73–80

Matsuyama Y, Uchida T, Honjo T, et al. 2001. Impacts of the harmful dinoflagellate, Heterocapsa circularisquama, on shellfish aquaculture in Japan. Journal of Shellfish Research, 20(3): 1269–1272

Miyazaki Y, Nakashima T, Iwashita T, et al. 2005. Purification and characterization of photosensitizing hemolytic toxin from harmful red tide phytoplankton, Heterocapsa circularisquama. Aquatic Toxicology, 73(4): 382–393

Morrill L C, Loeblich Ⅲ A R. 1981. A survey for body scales in dinoflagellates and a revision of Cachonina and Heterocapsa (Pyrrhophyta). Journal of Plankton Research, 3(1): 53–65

Morrill L C, Loeblich Ⅲ A R. 1983. Formation and release of body scales in the dinoflagellate genus Heterocapsa. Journal of the Marine Biological Association of the United Kingdom, 63(4): 905–913

Nishiguchi T, Cho K, Yasutomi M, et al. 2016. Intracellular haemolytic agents of Heterocapsa circularisquama exhibit toxic effects on H. circularisquama cells themselves and suppress both cell-mediated haemolytic activity and toxicity to rotifers (Brachionus plicatilis). Aquatic Toxicology, 179: 95–102

Pennick N C, Clarke K J. 1977. The occurrence of scales in the peridinian dinoflagellate Heterocapsa triquetra (Ehrenb.) Stein. British Phycological Journal, 12(1): 63–66

Rintala J M, Hällfors H, Hällfors S, et al. 2010. Heterocapsa arctica subsp. Frigida subsp. nov. (Peridiniales, Dinophyceae)–description of a new dinoflagellate and its occurrence in the Baltic Sea. Journal of Phycology, 46(4): 751–762

Roberts K R, Timpano P, Montegut A E. 1987. The apical pore fibrous complex: a new cytological feature of some dinoflagellates. Protoplasma, 137(1): 65–69

Salas R, Tillmann U, Kavanagh S. 2014. Morphological and molecular characterization of the small armoured dinoflagellate Heterocapsa minima (Peridiniales, Dinophyceae). European Journal of Phycology, 49(4): 413–428

Salcedo T, Upadhyay R J, Nagasaki K, et al. 2012. Dozens of toxin-related genes are expressed in a nontoxic strain of the dinoflagellate Heterocapsa circularisquama. Molecular Biology and Evolution, 29(6): 1503–1506

Sato Y, Oda T, Muramatsu T, et al. 2002. Photosensitizing hemolytic toxin in Heterocapsa circularisquama, a newly identified harmful red tide dinoflagellate. Aquatic Toxicology, 56(3): 191–196

Stern R F, Andersen R A, Jameson I, et al. 2012. Evaluating the ribosomal internal transcribed spacer (ITS) as a candidate dinoflagellate barcode marker. PLoS One, 7(8): e42780

Tamura M, Iwataki M, Horiguchi T. 2005. Heterocapsa psammophila sp. nov. (Peridiniales, Dinophyceae), a new sand-dwelling marine dinoflagellate. Phycological Research, 53(4): 303–311

Tamura K, Stecher G, Peterson D, et al. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725–2729

Toriumi S, Dodge J D. 1993. Thecal apex structure in the Peridiniaceae (Dinophyceae). European Journal of Phycology, 28(1): 39–45

Wang Jinhui, Qin Yutao, Liu Caicai, et al. 2006. The preliminary investigation of potentially toxic algae and bio-toxin in Changjiang Estuary. Marine Environmental Science (in Chinese), 25(S1): 15–19

White T J, Bruns T, Lee S, et al. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M A, Gelfand D H, Sninsky J J, et al, eds. PCR Protocols: A Guide to Methods and Applications. San Diego: Academic Press, 315–322

Yang Xia, Sun Na, Li Yonghan, et al. 2015. Effects of two harmful species algae on reproduction of rotifer Brachionus plicatilis and metamorphosis of zoea of Chinese mitten handed crab Eriocheir sinensis. Journal of Dalian Ocean University (in Chinese), 30(4): 351–356

Zheng Junbin. 2009. Molecular identification of Karenia mikimotoi and Heterocapsa circularisquama (in Chinese) [dissertation]. Shanghai: Shanghai Ocean University

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