Blooms of <i>Prorocentrum donghaiense</i> reduced the species diversity of dinoflagellate community

Huan Wang; Zhangxi Hu; Zhaoyang Chai; Yunyan Deng; Zifeng Zhan; Ying Zhong Tang

doi:10.1007/s13131-020-1585-1

Volume 39 Issue 4

Apr. 2020

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Huan Wang, Zhangxi Hu, Zhaoyang Chai, Yunyan Deng, Zifeng Zhan, Ying Zhong Tang. Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community[J]. Acta Oceanologica Sinica, 2020, 39(4): 110-119. doi: 10.1007/s13131-020-1585-1

Citation:

Huan Wang, Zhangxi Hu, Zhaoyang Chai, Yunyan Deng, Zifeng Zhan, Ying Zhong Tang. Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community[J]. Acta Oceanologica Sinica, 2020, 39(4): 110-119. doi: 10.1007/s13131-020-1585-1

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Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community

doi: 10.1007/s13131-020-1585-1

Huan Wang^{1, 3},
Zhangxi Hu^{1, 2, 4},
Zhaoyang Chai^{1, 2, 4},
Yunyan Deng^{1, 2, 4},
Zifeng Zhan⁵,
Ying Zhong Tang^{1, 2, 4
,
,}

1.
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
3.
University of Chinese Academy of Sciences, Beijing 100049, China
4.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
5.
Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Funds: The National Natural Science Foundation of China under contract Nos 61533011 and 41776125; the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences under contract No. U1606404; the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology under contract No. 2016ASKJ02; the National Key R&D Program of China under contract No. 2017YFC1404300; the Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology under contract No. LMEES-CTSP-2018-1.

More Information

Corresponding author: E-mail: yingzhong.tang@qdio.ac.cn
Received Date: 2018-11-24
Accepted Date: 2019-02-19

Available Online: 2020-12-28

Publish Date: 2020-04-25

Abstract

Abstract

Most of reported harmful algal blooms (HABs) of microalgae (75%) have been caused by dinoflagellates. Studies on the negative effects of HABs have generally focused on animals, valuable organisms in particular, and environmental factors such as dissolved oxygen and nutrients, but relatively fewer on community level, particularly that using metagenomic approach. In this study, we reported an investigation on the effects of a HAB caused by the dinoflagellate Prorocentrum donghaiense on the species diversity and community structure of the dinoflagellate sub-community via a pyrosequencing approach for the samples taken before, during, and after the bloom season of P. donghaiense in the East China Sea. We sequenced partial 28S rRNA gene of dinoflagellates for the field samples and evaluated the species richness and diversity indices of the dinoflagellate community, as a sub-community of the total phytoplankton. We obtained 800 185 valid sequences (categorized into 560 operational taxonomic units, OTUs) of dinoflagellates from 50 samples and found that the biodiversity of dinoflagellate community was significantly reduced during the blooming period in comparison to that in pre- and after-blooming periods, as reflected in the four diversity indices: the species richness expressed as the number of OTUs, Chao1 index, Shannon index (evenness), and Gini-Simpson index. These four indices were all found to be negatively correlated to the cell density of the bloom species P. donghaiense. Correlation analyses also revealed that the P. donghaiense cell abundance was correlated negatively with ${\rm{NO}}_3^- $-N, and ${\rm{NO}}_2^- $-N, but positively with total nitrogen (TN) and total phosphorus (TP). Principal coordinates analysis (PCoA) showed that the community structure of dinoflagellates was markedly different among the different sampling periods, while the redundancy analysis (RDA) revealed P. donghaiense abundance, salinity, ${\rm{NO}}_3^- $-N, and ${\rm{SiO}}_3^{2-} $ were the most four significant factors shaping the dinoflagellate community structure. Our results together demonstrated that HABs caused by the dinoflagellate P. donghaiense could strongly impact the aquatic ecosystem on the sub-community level which the blooming species belongs to.
- Prorocentrum donghaiense,
- dinoflagellate community,
- diversity,
- pyrosequencing,
- East China Sea

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References(40)

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