Parentage determination of black sea bream (<i>Acanthopagrus schlegelii</i>) for stock enhancement: effectiveness and loss of genetic variation

Binbin Shan; Yan Liu; Na Song; Changping Yang; Shengnan Liu; Tianxiang Gao; Dianrong Sun

doi:10.1007/s13131-020-1697-7

Volume 40 Issue 6

Jun. 2021

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Article Navigation > Acta Oceanologica Sinica > 2021 > 40(6): 41-49

Binbin Shan, Yan Liu, Na Song, Changping Yang, Shengnan Liu, Tianxiang Gao, Dianrong Sun. Parentage determination of black sea bream (Acanthopagrus schlegelii) for stock enhancement: effectiveness and loss of genetic variation[J]. Acta Oceanologica Sinica, 2021, 40(6): 41-49. doi: 10.1007/s13131-020-1697-7

Citation:

Binbin Shan, Yan Liu, Na Song, Changping Yang, Shengnan Liu, Tianxiang Gao, Dianrong Sun. Parentage determination of black sea bream (Acanthopagrus schlegelii) for stock enhancement: effectiveness and loss of genetic variation[J]. Acta Oceanologica Sinica, 2021, 40(6): 41-49. doi: 10.1007/s13131-020-1697-7

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Parentage determination of black sea bream (Acanthopagrus schlegelii) for stock enhancement: effectiveness and loss of genetic variation

doi: 10.1007/s13131-020-1697-7

Binbin Shan^{1, 2, 3, 4},
Yan Liu^{1, 2, 3, 4},
Na Song⁵,
Changping Yang^{1, 2, 3, 4},
Shengnan Liu^{1, 2, 3},
Tianxiang Gao^{6
,
,},
Dianrong Sun^{1, 2, 3, 4
,
,}

1.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture Rural Affairs, Guangzhou 510300, China
2.
Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China
3.
Scientific Observation and Research Field Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangzhou 510300, China
4.
South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510300, China
5.
Fishery College, Ocean University of China, Qingdao 266003, China
6.
Fishery College, Zhejiang Ocean University, Zhoushan 316022, China

Funds: The Science and Technology Project of Guangdong Province under contract No. 2019B121201001; the National Key R&D Program of China under contract No. 2019YFD0901301; the Fund of China-Vietnam Joint Survey on Fish Stocks in the Common Fishing Zone of the Beibu Gulf; the Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science under contract No. 2021SD14; the China-ASEAN Maritime Cooperation Fund (China-Vietnam Fishery Stock Enhancement and Conservation in Beibu Gulf); the Hainan Provincial Natural Science Foundation of China under contract No. 320QN358.

More Information

Corresponding author: Tianxiang Gao, gaotx9999@163.com; Dianrong Sun, sundianrong@yeah.net
Received Date: 2019-11-28
Accepted Date: 2020-06-03

Available Online: 2021-06-21

Publish Date: 2021-06-01

Abstract

Abstract

The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a few years. However, little information has been reported concerning the effectiveness and genetic effect of black sea bream stock enhancement. In order to detect the contribution of released individuals in Zhujiang River Estuary (ZRE) and Daya Bay (DB), six microsatellite markers were used to identify the hatchery-released individuals. In addition, this pedigree of hatchery populations (broodfish and hatchery-released offspring) was traced to detect the number of effective parents (N_e), the inbreeding coefficient and the decrease of genetic variability in the reproduction. The pedigree reconstruction showed that at least 69 (out of 93) broodfish had offspring. The estimated N_e was 54.8, consequently the inbreeding coefficient was 0.91%. The genetic diversity of hatchery-released offspring was lower than that in that of broodfish (heterozygosity alleles, 0.727–0.774), some alleles (number of alleles, 61–69) and genetic variance were lost during reproduction. It was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area. A total of 128 hatchery-released black sea bream were identified among 487 recaptured samples in ZRE, while a total of 15 samples were identified among 96 samples in DB. In summary, there was a high survival of released fish. Nevertheless, the results provided evidence to consider a loss of genetic variation in hatchery-released stock and a negative genetic effect of the stock enhancement.
- Acanthopagrus schlegelii,
- stock enhancement effectiveness,
- microsatellite maker,
- genetic effect,
- pedigree of hatchery populations

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References(38)

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