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Volume 40 Issue 9
Sep.  2021
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Article Contents
Abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
Article Navigation >  Acta Oceanologica Sinica  > 2021  >  40(9): 43-52
Jian Zheng, Bingjie Chen, Tianxiang Gao, Na Song. The mitochondrial genome of Chaeturichthys stigmatias provides novel insight into the interspecific difference with Amblychaeturichthys hexanema[J]. Acta Oceanologica Sinica, 2021, 40(9): 74-81. doi: 10.1007/s13131-021-1787-1
Citation: Lianhua He, Jihua Liu, Hui Zhang, Jingjing Gao, Aimei Zhu, Ying Zhang. Copper and zinc isotope variations in ferromanganese crusts and their isotopic fractionation mechanism[J]. Acta Oceanologica Sinica, 2021, 40(9): 43-52. doi: 10.1007/s13131-021-1775-5
shu

Copper and zinc isotope variations in ferromanganese crusts and their isotopic fractionation mechanism

doi: 10.1007/s13131-021-1775-5
  • 1.

    Key Laboratory of Marine Geology and Metallogeny, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China

  • 2.

    Laboratory for Marine Geology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266061, China

Funds:  The Shandong Provincial Natural Science Foundation of China under contract No. ZR2014DP009; the China Ocean Mineral Resource Research and Development Association Research Program under contract Nos DY135-N-1-03, DY135-C1-1-04 and DY135-R2-1-03; the Fund of the Construction and Operation of Test and Technical Support System for Natural Resources Investigation and Evaluation.
More Information
  • Corresponding author: E-mail: jihliu@fio.org.cn
  • Received Date: 2020-06-02
  • Accepted Date: 2020-09-27
    • Available Online: 2021-07-27
  • Publish Date: 2021-09-30
    Abstract
  • Ferromanganese (Fe-Mn) crusts are potential archives of the Cu and Zn isotope compositions of seawater through time. In this study, the Cu and Zn isotopes of the top surface of 28 Fe-Mn crusts and 2 Fe-Mn nodules were analysed by MC-ICP-MS using combined sample-standard bracketing for mass bias correction. The Zn isotope compositions of the top surface of Fe-Mn crusts are in the range of 0.71‰ to 1.08‰, with a mean δ66Zn value of 0.94‰±0.21‰ (2SD, n=28). The δ65Cu values of the top surface of Fe-Mn crusts range from 0.33‰ to 0.73‰, with a mean value of 0.58‰±0.20‰ (2SD, n=28). The Cu isotope compositions of Fe-Mn crusts are isotopically lighter than that of dissolved Cu in deep seawater (0.58‰ vs. 0.9‰). In contrast, the δ66Zn values of Fe-Mn crusts appear to be isotopically heavy compared to deep seawater (0.94‰±0.21‰ vs. 0.51‰±0.14‰). The isotope fractionation between Fe-Mn crusts and seawater is attributed to equilibrium partitioning between the sorption to crusts and the organic-ligand-bound Cu and Zn in seawater. The Cu and Zn isotopes in the top surface of Fe-Mn crusts are not a direct reflection of the Cu and Zn isotopes, but a function of Cu and Zn isotopes in modern seawater. This study proposes that Fe-Mn crusts have the potential to be archives for paleoceanography through Cu and Zn isotope analysis.
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  • Chaeturichthys stigmatias and Amblychaeturichthys hexanema, belong to the Gobiiade, Perciforms (Wu and Zhong, 2008). They are offshore warm fish species which are widely distributed in Korea, Japan and China. Chaeturichthys stigmatias mainly inhabits the sandy-mud bottom and lives on benthonic fauna (Chen et al., 2016). It was reported to be the dominant species in the Huanghe River Estuary and its adjacent waters (Meng et al., 2017). Amblychaeturichthys hexanema mainly inhabits the shallow and estuarine waters (Li et al., 2000) and lives on more than 40 kinds of food organisms (Han et al., 2013).

    The morphological characters like body shape and color of C. stigmatias are similar to A. hexanema (Nelson et al., 2006), and then they are often wrongly classified. Both species roughly have the same number of fin ray and fin spines on their first dorsal fin. Besides, they have 3−4 pairs of short tentacles on the chin of both species, which are different from most other gobies. The dorsal fins of these two species have scattered black stripes, while C. stigmatias has a black spot on the posterior of the first dorsal fin (Wu and Zhong, 2008). However, this characteristic is often not obvious enough to distinguish them. Previous ichthyologic researches had placed them into the Chaeturichthys genus (East China Sea Fishery Research Institute of Chinese Academy of Fishery Sciences, 1990). Qu (2018) thought that C. stigmatias and A. hexanema were very similar in morphology and showed closely genetic relationship in morphological tree among 28 goby fish. The otolith morphology of the two species was also relatively similar, and it was difficult to distinguish them (Yu, 2014).

    There have been abundant reports on morphology, otolith, ingestion of C. stigmatias and A. hexanema until now (Mei et al., 2010; Han et al., 2013; Meng et al., 2017). However, few genetic studies of C. stigmatias and A. hexanema were conducted. The complete mitochondrial sequences of C. stigmatias (KC495071.1) and A. hexanema (KT781104) were determined (Sun et al., 2015; Jin et al., 2012), however, two complete mitochondrial genomes showed high homology. The structures of mtDNA D-loop region and mitochondrial cytochrome c oxidase subunit I (COI) sequence between the two sequences are also basically the same. Therefore, these two species may have been misidentified.

    The molecular tools like DNA barcoding are often required to identify marine fish because the morphological characters of many species are too similar to identify (Smith et al., 2008). The mitochondrial COI gene, usually used as DNA barcoding, has been widely used in species identification (Wang et al., 2018; Puckridge et al., 2013), description of new record species or new species (Guo et al., 2010; Gao et al., 2011) and discovery of cryptic species (Zemlak et al., 2009; Suh et al., 2019). Besides, 12S rRNA gene has been recently developed as DNA mini-barcoding. It can be used in environmental samples needing universal primers, processed biological material such as food products and archival specimens of DNA degradation because it has shorter length and can be more efficient amplification (Meusnier et al., 2008; Shokralla et al., 2015). Masaki et al. (2015) aligned whole 12S rRNA sequences from 180 species and designed a set of universal PCR primers (MiFish-U/E) of fish, and they have been widely used for identification of marine fish.

    This study re-determined the complete mitochondrial genome of C. stigmatias and analyzed the interspecific difference between C. stigmatias and A. hexanema by COI gene and 12S rRNA gene. This study is conducive to reconstruct the phylogeny of the Gobiiade and can provide effective basic data for two goby species.

    2.1 Sample collection

    A total of 30 ind. of C. stigmatias and 24 ind. of A. hexanema from the seacoast of Qingdao, Shandong Province, China, were collected in this study. The information of samples was shown in Table 1. The two goby species were initially identified by the morphological characters like black spot on the first dorsal fin, and then COI and 12S rRNA sequences were amplified and analyzed. A piece of skeletal muscle of these samples was excised for DNA extraction and preserved in 95% ethanol until use. The COI sequences and 12S rRNA sequences of 30 ind. of C. stigmatias and 24 ind. of A. hexanema were amplified for studying the interspecific difference. One ind. of C. stigmatias was used for determining its complete mitochondrial genome sequence.

    Table  1.  The sampling information of C. stigmatias and A. hexanema
    SpeciesSampling siteSampling timeSample number/ind.Body length/mmWeight/g
    C. stigmatiasQingdaoDec. 20183094.75−196.174.4−39.4
    A. hexanemaQingdaoDec. 20182467.63−133.062.7−20.3
     | Show Table
    DownLoad: CSV

    2.2 DNA extraction, PCR amplification and sequencing

    Total genomic DNA was isolated from the muscle tissue by proteinase K digestion followed by the standard phenol/chloroform method (Sambrook et al., 1989).

    Primers used for the amplification of the COI gene were F1-5′-TCAACCAACCACAAAGACATTGGCAC-3′ and R1-5′-TAGACTT CTGGGTGGCCAAAGAATCA-3′ (Ward et al., 2008), and the 12S rRNA sequences were amplified by primers, MiFish-U-F: 5′-GTCGGTAAAACTCGTGCCAGC-3′ and MiFish-U-R: 5′-CATAGTGGGGTATCTAATCCCAGTTTG-3′ (Masaki et al., 2015).

    All PCRs were performed in an Eppendorf thermal cycler (A300 Fast Thermal Cycler). TaKaRa Ex Taq were used for polymerase chain reaction (PCR). PCR was carried out in 25 µL volumes containing 0.15 µL Taq DNA polymerase, 1 µL template DNA, 2.5 µL 10× DNA loading buffer, 2 µL dNTP, 1 µL forward and reverse primer. The PCR amplification was carried out under the following conditions: 3 min initial denaturation at 95°C, and 40 cycles of 45 s at 94°C for denaturation, 45 s at 52°C for annealing, and 45 s at 72°C for extension, and a final extension at 72°C for 5 min (Liu et al., 2007).

    Subsequent complete sequence of C. stigmatias was accomplished by primer walking method. Thirty-five normal PCR primer sets were designed to accomplish the entire mitogenome (Table A1). It was essential that every two contiguous segments overlapped by at least 50 bp to ascertain the accuracy of sequencing. The PCR amplification process was the same as above except for the annealing temperature. The annealing temperature of primers were shown in Table A1.

    All PCR products were detected by 2% agarose gel electrophoresis, the products with good amplification effect were sent to Tsingke Biotech Co., Ltd. (China) for sequencing. To ensure the accuracy of the sequences, each sample was sequenced in both directions.

    2.3 Sequence analysis

    The obtained sequences were analyzed by DNASTAR software (Madison, USA). ARLEQUIN 3.5 was used to calculate haplotype diversity (h), and nucleotide diversity (π) (Excoffier et al., 2005). Nucleotide composition and genetic distance were analyzed using MEGA 5 based on the Kimura 2-parameter model (Tamura et al., 2011).

    The mitochondrial genome sequence alignment was performed initially using DNASTAR software (Madison, USA) with default parameters, and further adjusted manually (Burland, 2000). Locations for protein-coding genes and rRNAs were determined by DOGMA (Wyman et al., 2004) with default settings.

    Phylogenetic analysis was constructed by Neighbor-joining (NJ) analysis with MEGA 5.0 (Saitou and Nei, 1987; Tamura et al., 2011). The bootstrap values in NJ analysis was 1 000 (Felsenstein, 1985).

    3.1 The interspecific difference between C. stigmatias and A. hexanema

    In this study, the length of COI homologous fragments of C. stigmatias and A. hexanema was 622 bp (GenBank accession: MT568667-MT568696 and MT568697-MT568720). The average base composition content of C. stigmatias was 23.6% for A, 26.9% for C, 18.2% for G, 31.3% for T, and A. hexanema was 23.8% for A, 28.0% for C, 19.0% for G, 29.2% for T. The content of C+G was slightly higher than that of C. stigmatias.

    A total of 9 polymorphic sites, 7 transition and 2 transversion were detected in 30 ind. of C. stigmatias. These sequences could be defined as 11 haplotypes. Besides, a total of 13 polymorphic sites, 11 transition and 2 transversion were detected in 24 ind. of A. hexanema, and 7 haplotypes were defined by these sites. No base insertions or deletions were detected within the two species.

    The number of haplotype and haplotype diversity of two gobies obtained based on the COI gene were shown in Table 2. The genetic diversity parameters of C. stigmatias were higher than those of A. hexanema. The intraspecific genetic distance of A. hexanema and C. stigmatias was 0.002 and 0.003, respectively, and the intraspecific genetic distance between two species was 0.187. Clustering analysis of COI haplotypes was conducted using NJ method (Fig. 1). The result showed that the two species clustered separately, without sharing haplotype.

    Table  2.  Genetic diversity parameters of C. stigmatias and A. hexanema based on COI gene
    ParameterA. hexanemaC. stigmatias
    Sample number/ind.2430
    Haplotypes number/ind. 711
    Haplotype diversity0.445 7±0.126 00.564 4±0.037 6
    Nucleotide diversity0.002 0±0.001 40.002 9±0.001 9
     | Show Table
    DownLoad: CSV
    Figure  1.  Neighbor-joining tree for COI haplotypes of A. hexanema and C. stigmatias. Bootstrap values of >70% (out of 1 000 replicates) are shown on the nodes.
    DownLoad: Full-Size Img PowerPoint

    A total of 194 bp 12S rRNA gene homologous fragments of C. stigmatias and A. hexanema were amplified (GenBank accession: MT614220-MT614249 and MT614190-MT614213). The contents of A, T, C and G of C. stigmatias and A. hexanema were 20.6%, 34.9%, 20.3%, 24.2% and 20.8%, 32.5%, 22.2%, 24.5%, respectively. The content of A+T was slightly higher than that of G+C, which was similar to the characteristics of higher A+T content in other fishes (Sun et al., 2012; Liu et al., 2012).

    A total of 1 polymorphic site was found within the 12S rRNA sequences. Two haplotypes were defined by these sites in the 30 ind. of C. stigmatias (Table 3). There was 1 polymorphic site in the 12S rRNA sequences of 24 ind. A. hexanema. This site could be defined as 2 haplotypes (Table 3). The NJ phylogenetic tree constructed by haplotypes of this two species showed that the two species could be clearly divided into two branches, which was consistent with the analysis results of the COI sequences (Fig. 2).

    Table  3.  Genetic diversity parameters of C. stigmatias and A. hexanema based on 12S rRNA gene
    ParameterA. hexanemaC. stigmatias
    Sample number/ind.2430
    Haplotypes number/ind. 2 2
    Haplotype diversity0.507 2±0.044 50.516 9±0.031 4
    Nucleotide diversity0.002 6±0.002 60.002 7±0.002 6
     | Show Table
    DownLoad: CSV
    Figure  2.  Neighbor-joining tree for 12S rRNA haplotypes of A. hexanema and C. stigmatias.
    DownLoad: Full-Size Img PowerPoint

    3.2 Complete sequence analysis

    The mitochondrial genome of C. stigmatias was acquired (MN038166), and it was 17 134 bp in length, covering 13 protein-coding genes, 22 tRNA, 2 rRNA genes, 1 OL and 1 D-loop region (Fig. 3). The overall nucleotide composition was 28.2% for A, 27.7% for T, 28.0% for C, and 16.1% for G, with a slight AT bias of 55.9%, which was similar to other goby fishes (Jin et al., 2012). In 13 protein-coding genes, the longest one was ND5 (1 842 bp in length), whereas the shortest one was ATP8 (165 bp in length). The lengths of tRNA genes were ranged from 64 bp (tRNA-Cys) to 74 bp (tRNA-Val, tRNA-Asn). As other vertebrates, with the exception of ND6 and 8 tRNAs (tRNA-Ala, tRNA-Asn, tRNA-Cys, tRNA-Gln, tRNA-Tyr, tRNA-Pro, tRNA-Glu, tRNA-Ser), all of the observed mitochondrial genes were found to be encoded on H strand.

    Figure  3.  The gene map of the complete mitochondrial genome of C. stigmatias. Genes encoded on the H-strand and L-strand are shown outside and inside the circular map of the mitogenome, respectively.
    DownLoad: Full-Size Img PowerPoint

    All the protein-coding genes started with the ATG codon except for COI and ND6, that started with GTG and CTA, respectively. Two types of stop codons were used by the coding genes: TAA (ND1, ND2, COI, ATP8, ATP6, ND4L, and ND5) and incomplete stop codon T (COII, COIII, ND3, ND4, and ND6). The control region (D-loop) was 992 bp sequence located between the tRNA-Pro and tRNA-Phe genes. The 12S rRNA and 16S rRNA were located between the tRNA-Phe and tRNA-Leu genes and separated by the tRNA-Val gene. Sequence analysis showed that many overlaps between genes (Table 4).

    Table  4.  Complete mitochondrial genome characteristics of C. stigmatias
    GeneStart position/bpStop position/bpSize/bpIntergenic nucleotideStart codonStop codonStrand
    tRNA-Phe168680−−H
    12S rRNA691 0179490−−H
    tRNA−Val1 0181 091740−−H
    16S rRNA1 0922 7741 6830−−H
    tRNA−Leu2 7752 847730−−H
    ND12 8483 8229753ATGTAAH
    tRNA−Ile3 8263 89570−1−−H
    tRNA−Gln3 8953 964701−−L
    tRNA−Met3 9664 033681−−H
    ND24 0355 0791 044−2ATGT − −H
    tRNA−Trp5 0785 149720−−H
    tRNA−Ala5 1505 221720−−L
    tRNA−Asn5 2225 295741−−L
    tRNA−Cys5 3275 390640−−L
    tRNA−Tyr5 3915 461713−−L
    COXI5 4657 0181 5540GTGTAAH
    tRNA−Ser7 0197 089713−−L
    tRNA−Asp7 0937 163712−−H
    COXII7 1667 8566911ATGT − −H
    tRNA−Lys7 8587 931740−−H
    ATP87 9328 096165−5ATGTAAH
    ATP68 0908 7736840ATGTAAH
    COXIII8 7749 5577840ATGT − −H
    tRNA−Gly9 5589 629720−−H
    ND39 6309 9783490ATGT − −H
    tRNA−Arg9 97910 046680−−H
    ND4L10 04710 3432970ATGTAAH
    ND410 34411 7241 3810ATGT − −H
    tRNA−His11 72511 79470−1−−H
    tRNA−Ser11 79411 861685−−H
    tRNA−Leu11 86711 939730−−H
    ND511 94013 7811 842−1ATGTAAH
    ND613 78114 7921 0120CTAT − −L
    tRNA−Glu14 79314 863710−−L
    Cyt b14 86416 0001 1375ATGTAAH
    tRNA−Thr16 00116 07272−1−−H
    tRNA−Pro16 07216 142710−−L
    D−loop16 14317 134992−−−H
    Note: H represents genes encoded on the H-strand; L, genes encoded on L-strand. −, no data.
     | Show Table
    DownLoad: CSV

    Phylogenetic tree based on the tandem 13 coding protein genes nucleotide sequences was constructed by NJ method (Fig. 4). From the tree topologies, this study could conclude that C. stigmatias was genetically closest to A. hexanema, and then to Oxyurichthys formosanus among 12 species within Gobioidei.

    Figure  4.  The phylogenetic analyses investigated using Neighbor-Joining based on nucleotide sequences of 13 concatenated protein-coding genes. Cyprinus carpio (GenBank: KP993139) was used as an outgroup. The black box represented sequences of C. stigmatias sequenced in this study.
    DownLoad: Full-Size Img PowerPoint

    DNA barcoding developed in recent years is an effective and efficient molecular tool used in species identification, especially for closely related fish species (Stoeckle, 2003; Hebert et al., 2003a, b). For example, Ward et al. (2005) analyzed the COI gene of 270 species of marine fish and resulted in all fish species could be discriminated by the COI gene. Compared with the conventional DNA barcoding, mini-barcoding like 12S rRNA barcoding was developed recently. It has shorter length and can be efficiently amplified, which can overcome some problems existing in traditional DNA barcoding, such as DNA degradation in biological food products and old archival specimens, high sequence variability in environmental samples (Meusnier et al., 2008; Shokralla et al., 2015).

    A1.  Primers for complete mitochondrial genome of C. stigmatias
    Primer name Primer sequence (5′−3′) Temperature/°C Reference
    MiFish-U F: GTCGGTAAAACTCGTGCCAGC 52 Miya et al. (2015)
    R: CATAGTGGGGTATCTAATCCCAGTTTG
    C1 F: AACCCTATTCACAGCAACAT 48 this study
    R: TGAGCAGTCCTAAGATAGTAAC
    C2 F: GCCCTAACAACCCTATTCA 52 this study
    R: AAGGCGACGATGAAGATG
    C3 F: GGTAAGGTTCTGCTGCTAA 50 this study
    R: ATCGGCGGTGTAGTGTAT
    16S F: GGTTTGAACTCAGATCATGT 52 Miya et al. (2015)
    R: GGTTTGAACTCAGATCATGT
    C4 F: TGCGAGCATGATACCATAA 52 this study
    R: GATTATCATCCGCCTATGCTA
    C5 F: CCAACATCTGAAGGTCCAC 48 this study
    R: GGTACGAAGATTAGCAGTCT
    C6 F: TCCTTAATTGCCTACTCATCC 48 this study
    R: GGTACGAAGATTAGCAGTCT
    C7 F: TAACAGGCATTGGCACTC 52 this study
    R: GTCAGCGAACTCAAGAATAG
    C8 F: AGACTGCTAATCTTCGTACC 54 this study
    R: GTTTGCTGTGACAAGAATGA
    C9 F: CACTTATAACCGCATGATGG 50 this study
    R: GGTACGAAGATTAGCAGTCT
    C10 F: GCCACATAAGCCTCGTAG 48 this study
    R: CCATCAGCCAATGAGTAAGA
    COI F: TCAACCAACCACAAAGACATTGGCAC 52 Ward et al. (2008)
    R: TAGACTTCTGGGTGGCCAAAGAATCA
    C11 F: GCCACCAACCTTAACTCTT 50 this study
    R: ACTCCATCTCAAGCAATCAG
    C12 F: GGCGACATCGGACTAATC 48 this study
    R: GGTATTGCTTGCTGTTGTT
    C13 F: CACTGATTGCTTGAGATGG 54 this study
    R: TGCGAGGAATGTAGTTAGG
    C14 F: CATAGGATAGCCATCAACCA 52 this study
    R: TGTCAGCGGTGTAGTGTA
    C15 F: TAACAACAGCAAGCAATACC 52 this study
    R: AGAAAATGTGTCCACGAGTT
    C16 F: GCAGTTTTACCAGCGAGC 52 this study
    R: GGCGATGTGTTGGGATGT
    C17 F: ACTTCGGTTCAAAGGTCAT 48 this study
    R: GCCGATGTGTTGGGATGTT
    C18 F: TTATGTTGAATAAGTTA 48 this study
    R: TATTACATAAAATTAACA
    C19 F: TATTACATAAATTTAACTAT 46 this study
    R: TCGGTCTGTAAGAAGCATAG
    C20 F: CTTCGGTTCAAAGGTCAT 54 this study
    R: GTAGTGGAGGTGAAGCGA
    C21 F: CAGGTAAAGCACAGTAGAGT 52 this study
    R: CCTTTCATTTGGCATTTCAG
    C22 F: TTACCAGCGAGCATCACCT 52 this study
    R: GTAGTGGAGGTGAAGCGAA
    C23 F: TTTTACCAGCGAGCATCACC 56 this study
    R: GTAGTGGAGGTGAAGCGAAA
    C24 F: AATCCAGGTCGGTTTCTATC 50 this study
    R: CTTGCTTGTGCGTGACTT
    C25 F: CAGGTCGGTTTCTATCTATG 48 this study
    R: TCTTCGCCCTTATTATCG
    C26 F: AGGTCGGTTTCTATCTATG 52 this study
    R: GTCCAGCCAAAGATAGATA
    C27 F: TTATGTTGAATAAGTTAATAT 48 this study
    R: AGAAAATGTGTCCACGAGTT
    C28 F: TATTACATAAAATTAACAATAA 52 this study
    R: GGCTACAATCCACCACTTA
    C29 F: CAGCGGTTCTTCTACTCTT 54 this study
    R: TCCTGCGAATATAACTCCAA
    C30 F: TCTCAACCACATTAACCGTA 48 this study
    R: CGTATAACAGCCTTGAAGATG
    C31 F: CTGTAGAGTGAACGCTTGGCATG 50 this study
    R: CCCATCTCTAGCTCCCAAAGC
    C32 F: GTGCGGATACTTGCATGTGT 52 this study
    R: TTAGCGCTGCACTCTGAAAT
     | Show Table
    DownLoad: CSV

    In this study, the result of conventional COI barcoding and 12S rRNA mini-barcoding analysis all indicated that there were significant genetic differences between C. stigmatias and A. hexanema, and the NJ tree based on COI and 12S rRNA haplotypes showed that the two species were clustered separately, which verified the validity of these two barcoding in interspecific identification. Therefore, combination of morphology and DNA barcoding to solve the problems of classification and identification will be more effective and accurate (Cywinska et al., 2006; Pfenninger et al., 2007).

    The mitochondrial genome has been widely used in evolutionary biology, population genetics and molecular ecology (Inoue et al., 2001; Miya et al., 2005; Lavoué et al., 2007). It has been proved that mitogenomic data can be used to resolve the controversial problems of phylogenetic relationships (Miya et al., 2003; Boore, 2006). In this study, the mitochondrial genome sequence of C. stigmatias was 17 134 bp, which was shorter than the sequence previously reported (Sun et al., 2015). This is due to the fact that there was repeat region in D-loop region of C. stigmatias previously reported. Some researchers have reported the COI sequence of C. stigmatias (Xing et al., 2018; Lu et al., 2016). These sequences were high homology with the COI sequences determined in this study, which showed significant difference with that in previous study. Therefore, C. stigmatias in previous study may have been misidentified as A. hexanema. The phylogenetic tree showed that the sequence of C. stigmatias clustered with most of Gobioidei. Phylogenetic analysis suggested that C. stigmatias had a close relationship with A. hexanema, which was consistent with the study result of morphological classification (Qu, 2018). The mitochondrial genome of C. stigmatias characterized in the present study would facilitate further studies on the genetic constitution of C. stigmatias population. It would also enrich essential information for investigating the biodiversity and conservation biology of C. stigmatias.

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