The application of inverse polarography with anti-adsorption physically coated mercury film electrode to analysis of fishes and benthos is described.The Zn, Cd, Pb and Cu concentrations of determined marine organisms are 3-212 μg/g, 0.3-7.2 μg/g, 0.1-7.5μg/g, and 0.06-8.8 μg/g respectively.