Phylogeny of the cuttlefishes (Mollusca: Cephalopoda) based on mitochondrial COI and 16S rRNA gene sequence data

To clarify cuttlefish phylogeny,mitochondrial cytochrome c oxidase subunit Ⅰ (COI) gene and partial 16S rRNA gene are sequenced for 13 cephalopod species.Phylogenetic trees are constructed,with the neighbor-joining method.Coleoids are divided into two main lineages,Decabrachia and Octobrachia.The monophyly of the order Sepioidea,which includes the families Sepiidae,Sepiolidae and Idiosepiidae,is not supported.From the two families of Sepioidea examined,the Sepiolidae are polyphyletic and are excluded from the order.On the basis of 16S rRNA and amino acid of COI gene sequences data,the two genera (Sepiella and Sepia) from the Sepiidae can be distinguished,but do not have a visible boundary using COI gene sequences.The reason is explained.This suggests that the 16S rDNA of cephalopods is a precious tool to analyze taxonomic relationships at the genus level,and COI gene is fitter at a higher taxonomic level (i.e.,family).