DONG Shirui, KONG Jie, ZHANG Qingwen, LIU Ping, MENG Xianhong, WANG Rucai. Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping[J]. Acta Oceanologica Sinica, 2006, (5): 151-157.
Citation:
DONG Shirui, KONG Jie, ZHANG Qingwen, LIU Ping, MENG Xianhong, WANG Rucai. Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping[J]. Acta Oceanologica Sinica, 2006, (5): 151-157.
DONG Shirui, KONG Jie, ZHANG Qingwen, LIU Ping, MENG Xianhong, WANG Rucai. Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping[J]. Acta Oceanologica Sinica, 2006, (5): 151-157.
Citation:
DONG Shirui, KONG Jie, ZHANG Qingwen, LIU Ping, MENG Xianhong, WANG Rucai. Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping[J]. Acta Oceanologica Sinica, 2006, (5): 151-157.
College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China;Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
2.
Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
3.
College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China
Two microsatellite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis.Four of the families were natural mating, and the others were mated by artificial insemination.Eleven alleles were acquired at two microsatellite DNA loci (locus RS0622 and locus EN0033) by investigating 145 offsprings and 11 parents.Five alleles were acquired from locus RS0622 and six from locus EN0033.As analyzed, the gene frequencies were between 0.024 1 and 0.493 1, the heterozygosity was 0.652 2 and 0.688 8, and the polymorphism information content (PIC) was 0.585 7 and 0.652 9 for the locus RS0622 and the locus EN0033, respectively.Twenty-three genotypes were detected and the genotypes of the losing parents were also inferred.The pedigrees of three F1 and three F2 generations were determined by matching the genotype at each locus.
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