Studies on the bioactivity of marine microorganisms using Pyricularia oryzae model

A total of 2 088 marine microorganisms including 1 392 strains of bacteria and 696 strains of fungi were isolated from diverse marine environment such as the South Pole, the North Pole, and Qingdao tideland, etc.Through a systematic screening process involving the use of Pyricularia oryzae mode, 90 bacteria and 31 fungi strains demonstrating positive bioactivities were identified.The rates of active strains of marine bacteria and marine fungi were 6.5% and 4.5%, respectively.The relationships between the active strains and the sampling locations and hosts were studied.For marine bacteria, the three locations showing the highest rates of active strains were 8.4% in the Zhujiang Estuary, 7.2% at the North Pole and 6.3% in the Bohai Sea.For marine fungi, the highest rates observed were 7.8% at the South Pole, 7.4% in the South China Sea and 6.8% at the Qingdao tideland.An analysis of the host showed that the three highest rates of active strain for marine bacteria were 8.2% for seawater from the Zhujiang Estuary, 8% for alga from the Qingdao tideland and 7.6% for sea mud from the North Pole.For marine fungi, the highest rates observed were 8.3% for Qingdao actiniae, 7.4% for South China Sea poriferan and 4.5% for soil of Qingdao Suaeda salsa.Four fungi and two bacteria samples were selected for further study because of their high bioactivity.They were found to be active towards several pathogenic microorganisms, and demonstrated stable genetic and thermal characteristics and conservation activities.