Development of a real-time PCR method for <i>Thalassiosira rotula</i> rapid detection

HE Shanying; YU Zhigang; MI Tiezhu

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Article Navigation > Acta Oceanologica Sinica > 2007 > (2): 133-139

HE Shanying, YU Zhigang, MI Tiezhu. Development of a real-time PCR method for Thalassiosira rotula rapid detection[J]. Acta Oceanologica Sinica, 2007, (2): 133-139.

Citation:

HE Shanying, YU Zhigang, MI Tiezhu. Development of a real-time PCR method for Thalassiosira rotula rapid detection[J]. Acta Oceanologica Sinica, 2007, (2): 133-139.

HE Shanying, YU Zhigang, MI Tiezhu. Development of a real-time PCR method for Thalassiosira rotula rapid detection[J]. Acta Oceanologica Sinica, 2007, (2): 133-139.

Citation:

HE Shanying, YU Zhigang, MI Tiezhu. Development of a real-time PCR method for Thalassiosira rotula rapid detection[J]. Acta Oceanologica Sinica, 2007, (2): 133-139.

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Development of a real-time PCR method for Thalassiosira rotula rapid detection

1.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China
2.
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, China

Received Date: 2006-03-07
Rev Recd Date: 2006-11-01

Abstract

Abstract

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula.A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula.The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.
- red tide,
- Thalassiosira rotula,
- fluorescent quantitative PCR,
- 18S rDNA