The point of no return and pectoral angle of Japanese anchovy(Engraulis japonicus) larvae during growth and starvation

At a temperature of 23.0~24.8℃, the mixed feeding of Japanese anchovy larvae was initiated 24 h before the yolk-sac was exhausted.The point of no return (PNR) was reached on the 6th day after hatching.On the 4th day after hatching, the pectoral angle appeared in both fed and unfed anchovy larvae although it was more evident and sharper in the starved and the PNR stage larvae than in the fed ones.According to observations of larvae collected in the sea, the pectoral angles were evident not only in the larvae of 3.62~7.44 mm in standard length, but also in the larvae of 2.70 mm in standard length with remnants of yolk.The pectoral angles became diffuse when the larvae reached 7.84 mm and vanished at 9.86 mm.The pectoral angle cannot be used as a criterion to distinguish healthy from starving larvae.