MA Jinyou, RUAN Lingwei, XU Xun, GAO Zhaoming. Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection[J]. Acta Oceanologica Sinica, 2016, 35(4): 44-50. doi: 10.1007/s13131-016-0835-8
Citation: MA Jinyou, RUAN Lingwei, XU Xun, GAO Zhaoming. Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection[J]. Acta Oceanologica Sinica, 2016, 35(4): 44-50. doi: 10.1007/s13131-016-0835-8

Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection

doi: 10.1007/s13131-016-0835-8
  • Received Date: 2014-10-23
  • Rev Recd Date: 2015-09-24
  • β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis, antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags(EST) from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 cDNAs possessed open reading frames that encoded 166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4, mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps. The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps.
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  • Bollini S, Riley P R, Smart N. 2015. Thymosin β4:multiple functions in protection, repair and regeneration of the mammalian heart. Expert Opinion on Biological Therapy, 15(S1):163-174
    Boquet I, Boujemaa R, Carlier M F, et al. 2000. Ciboulot regulates actin assembly during Drosophila brain metamorphosis. Cell, 102(6):797-808
    Chen Dandan, He Nanhai, Xu Xun. 2008. Mj-DWD, a double WAP domain-containing protein with antiviral relevance in Marsu-penaeus japonicus. Fish & Shellfish Immunology, 25(6):775-781
    Choi H M, Lee Y A, Yang H I, et al. 2011. Increased levels of thymosin β4 in synovial fluid of patients with rheumatoid arthritis:asso-ciation of thymosin β4 with other factors that are involved in in-flammation and bone erosion in joints. International Journal of Rheumatic Diseases, 14(4):320-324
    Crow T, Xue-Bian J J. 2000. Identification of a 24 kDa phosphopro-tein associated with an intermediate stage of memory in Her-missenda. Journal of Neuroscience, 20(10):RC74
    Dettin M, Ghezzo F, Conconi M T, et al. 2011. In vitro and in vivo pro-angiogenic effects of thymosin-β4-derived peptides. Cellular Immunology, 271(2):299-307
    Gai Yunchao, Zhao Jianmin, Song Linsheng, et al. 2009. Two thymos-in-repeated molecules with structural and functional diversity coexist in Chinese mitten crab Eriocheir sinensis. Development & Comparative Immunology, 33(7):867-876
    He Nanhai, Liu Haipeng, Xu Xun. 2004. Identification of genes in-volved in the response of haemocytes of Penaeus japonicus by suppression subtractive hybridization(SSH) following microbi-al challenge. Fish & Shellfish Immunology, 17(2):121-128
    Herrmann D, Hatta M, Hoffmeister-Ullerich S A H. 2005. Thypedin, the multi copy precursor for the hydra peptide pedin, is a β-thymosin repeat-like domain containing protein. Mechanisms of Development, 122(11):1183-1193
    Huff T, Müller C S G, Otto A M, et al. 2001. β-thymosins, small acidic peptides with multiple functions. The International Journal of Biochemistry & Cell Biology, 33(3):205-220
    Kasthuri S R, Premachandra H K A, Umasuthan N, et al. 2013. Struc-tural characterization and expression analysis of a beta-thymosin homologue(Tβ) in disk abalone, Haliotis discus dis-cus. Gene, 527(1):376-383
    Klein J J, Goldstein A L, White A. 1965. Enhancement of in vivo incor-poration of labeled precursors into DNA and total protein of mouse lymph nodes after administration of thymic extracts. Proceedings of the National Academy of Sciences of the United States of America, 53(4):812-817
    Liu Haipeng, S.derh.ll K, Jiravanichpaisal P. 2009. Antiviral im-munity in crustaceans. Fish & Shellfish Immunology, 27(2):79-88
    Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25(4):402-408
    Lv Shumin, Cheng Gang, Zhou Ying, et al. 2013. Thymosin beta4 in-duces angiogenesis through Notch signaling in endothelial cells. Molecular and Cellullar Biochemistry, 381(1-2):283-290
    Nam B H, Seo J K, Lee M J, et al. 2015. Functional analysis of Pacific oyster(Crassostrea gigas) β-thymosin:focus on antimicrobial activity. Fish & Shellfish Immunology, 45(1):167-174
    Romanova E V, Roth M J, Rubakhin S S, et al. 2006. Identification and characterization of homologues of vertebrate β-Thymosin in the marine mollusk Aplysia californica. Journal of Mass Spec-trometry, 41(8):1030-1040
    Saelee N, Noonin C, Nupan B, et al. 2013. β-thymosins and hemocyte homeostasis in a crustacean. PLoS One, 8(4):e60974
    Safer D, Chowrashi P K. 1997. β-Thymosins from marine inverteb-rates:primary structure and interaction with actin. Cell Motil-ity and Cytoskeleton, 38(2):163-171
    Sánchez-Paz A. 2010. White spot syndrome virus:an overview on an emergent concern. Veterinary Research, 41(6):43
    Shi Xiuzhen, Shi Lijie, Zhao Yanran, et al. 2015. β-Thymosins parti-cipate in antiviral immunity of red swamp crayfish(Procam-barus clarkii). Developmental & Comparative Immunology, 51(2):213-225
    Sosne G, Qiu Ping, Kurpakus-Wheater M, et al. 2010. Thymosin β4 and corneal wound healing:visions of the future. Annals of New York Academy of Sciences, 1194:190-198
    Sun Yumiao, Li Fuhua, Chi Yanhong, et al. 2013. Enhanced resist-ance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria. Acta Oceanologica Sinica, 32(2):52-58
    Tamura K, Dudley J, Nei M, et al. 2007. MEGA4:molecular evolution-ary genetics analysis(MEGA) software version 4.0. Molecular Biology and Evolution, 24(8):1596-1599
    Vancompernolle K, Vandekerckhove J, Bubb M R, et al. 1991. The in-terfaces of actin and Acanthamoeba actobindin. Identification of a new actin-binding motif. Journal of Biological Chemistry, 266(23):15427-15431
    Van Troys M, Ono K, Dewitte D, et al. 2004. Tetra thymosinbeta is re-quired for actin dynamics in Caenorhabditis elegans and acts via functionally different actin-binding repeats. Molecular Bio-logy of the Cell, 15(10):4735-4748
    Wang Wei, He Jun, Yang Feng, et al. 1999. Detection of prawn white spot baculovirus by polymerase chain reaction. Acta Oceanolo-gica Sinica, 18(4):591-598
    Wen Rong, Li Fuhua, Li Shihao, et al. 2014. Function of Shrimp STAT during WSSV infection. Fish & Shellfish Immunology, 38(2):354-360
    Wu Liuji, Wu Xinzhong. 2009. Molecular cloning and expression ana-lysis of a β-Thymosin homologue from a gastropod abalone, Haliotis diversicolor supertexta. Fish & Shellfish Immunology, 27(2):379-382
    Xiao Zhanggang, Shen Jing, Feng Hong, et al. 2015. Characterization of two thymosins as immune-related genes in common carp(Cyprinus carpio L.). Developmental & Comparative Immuno-logy, 50(1):29-37
    Yang Feng, He Jun, Lin Xionghui, et al. 2001. Complete genome se-quence of the shrimp white spot bacilliform virus. Journal of Virology, 75(23):11811-11820
    Zhu Tingting, Park H C, Son K M, et al. 2014. Effects of thymosin β4 on wound healing of rat palatal mucosa. International Journal of Molecular Medicine, 34(3):816-821
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