HUANG Bingxin, TENG Linhong, DING Lanping. Morphological and molecular discrimination of green macroalgae Chaetomorpha aerea and C. linum[J]. Acta Oceanologica Sinica, 2016, 35(4): 118-123. doi: 10.1007/s13131-016-0841-x
Citation: HUANG Bingxin, TENG Linhong, DING Lanping. Morphological and molecular discrimination of green macroalgae Chaetomorpha aerea and C. linum[J]. Acta Oceanologica Sinica, 2016, 35(4): 118-123. doi: 10.1007/s13131-016-0841-x

Morphological and molecular discrimination of green macroalgae Chaetomorpha aerea and C. linum

doi: 10.1007/s13131-016-0841-x
  • Received Date: 2015-01-13
  • Rev Recd Date: 2015-08-07
  • Green macroalgae Chaetomorpha aerea and C. linum are taxonomically confused. In this paper, we tried morphological and molecular analyses to separate these two species. C. aerea and C. linum can be distinguished from morphological characteritics, such as frond dimension, cells size and shape, their mean length/width ratios(LWR), and cell walls constriction. Thalli of C. aerea attenuate basipetally, with diameter 270-500μm at upper portion, 160-360μm at middle portion, 100-160μm at basal portion. For the upper part, the length of cells is less than their diameter. Cell walls usually constrict at the dissepiments, which are pellucid or colorless and give the filament beaded appearance. In contrast, thalli of C. linum often have a constant diameter of 90-300μm within the same individual, cell walls usually do not constrict and cells are cylindrical or barrel shaped. The LWR is larger than that of C. aerea. Results show that the pairwise distance between two species is 3.6%-3.7% for 18S rRNA gene and 53.5%-54.3% for ITS region. In phylogeny, they distribute at distant clades, which confirms a genetic divergence at molecular level. In addition, morphological data indicates that filament diameter of C. linum samples is highly variable, ranging from 90μm to 300μm. Then these two species can be considered as separate species.
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  • Bakker F T, Olsen J L, Stam W T, et al. 1992. Nuclear ribosomal DNA internal transcribed spacer regions(ITS1 and ITS2) define dis-crete biogeographic groups in Cladophora-albida(Chloro-phyta). Journal of Phycology, 28(6):839-845
    Bakker F T, Olsen J L, Stam W T, et al. 1994. The Cladophora complex(Chlorophyta):New views based on 18S rRNA gene sequences. Molecular Phylogenetics and Evolution, 3(4):365-382
    Bakker F T, Olsen J L, Stam W T. 1995a. Evolution of nuclear rDNA its sequences in the Cladophora albida/sericea clade(Chloro-phyta). Journal of Molecular Evolution, 40(6):640-651
    Bakker F T, Olsen J L, Stam W T. 1995b. Global phylogeography in the cosmopolitan species Cladophora vagabunda(Chlorophyta) based on nuclear rDNA internal transcribed spacer sequences. European Journal of Phycology, 30(3):197-208
    Blair S M. 1983. Taxonomic treatment of the Chaetomorpha and Rhizoclonium species(Cladophorales, Chlorophyta) in new-England. Rhodora, 85(842):175-211
    Blair S M, Mathieson A C, Cheney D P. 1982. Morphological and Elec-trophoretic investigations of selected species of Chaetomorpha(Chlorophyta; Cladophorales). Phycologia, 21(2):164-172
    Burrows E M. 1991. Seaweeds of the British Isles. Volume 2 Chloro-phyta. London:Natural History Museum Publications
    Chiang Y M. 1960. Marine algae of northern Taiwan(Cyanophyta, Chlorophyta, Phaeophyta). Taiwania, 7(1):51-75
    Christensen T. 1957. Chaetomorpha linum in the attached state. Bot-anisk Tidsskrift, 53:311-316
    Collins F S. 1909. The green algae of North America. Tufts College Studies, Scientific Series, 2(3):79-480
    Collins F S. 1918. The green algae of North America. Second supple-mentary paper. Tufts College Studies, Scientific Series, 4(7):1-106
    Dillwyn L W. 1806. British Conferv.; or, Colored Figures and De-scriptions of the British Plants Referred by Botanists to the Genus Conferva. London:W. Phillips
    Hall T A. 1999. BioEdit:a user-friendly biological sequence align-ment editor and analysis program for Windows 95/98/NT. Nuc-leic Acids Symposium Series, 41:95-98
    John D M, Lawson G W, Ameka G K. 2003. The Marine Macroalgae of the Tropical West Africa Sub-region:Beihefte zur Nova Hed-wigia. Berlin:J. Cramer, 125:1-217
    John D M, van Reine W F P, Lawson G W, et al. 2004. A Taxonomic and Geographical Catalogue of the Seaweeds of the Western Coast of Africa and Adjacent Islands:Beihefte zur Nova Hed-wigia. Berlin:J. Cramer, 127:1-339
    Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucle-otide sequences. Journal of Molecular Evolution, 16(2):111-120
    Kornmann P. 1972. Ein Beitrag zur Taxonomie der Gattung Chaeto-morpha(Cladophorales, Chlorophyta). Helgol.nder wis-senschaftliche Meeresuntersuchungen, 23(1):1-31
    Kützing F T. 1845. Phycologia Germanica, d.i. Deutschlands Algen in bündigen Beschreibungen. Nebst einer Anleitung zum Unter-suchen und Bestimmen dieser Gew.chse für Anf.nger.. Nord-hausen:W. K.hne, 1-340
    Kützing F T. 1849. Species Algarum. pp.[i]-vi,[1]-922. Lipsiae[Leipzig], Germany:F. A. Brockhaus
    Lawson G W, John D W. 1987. The marine algae and coastal environ-ment of tropical West Africa(Second Edition). Beihefte zur Nova Hedwigia, 93:vi + 1-415
    Li R G. 1964. Marine Green algae of Lüda, Liaoning Province. Jilin Normal University Journal(in Chinese),(1):91-106
    Luan R X. 1989. Field Guide of Marine Algae Along Dalian Coast(in Chinese). Dalian:Dalian Maritime University Press, 1-129
    Mathieson A C, Norton T A, Neushul M. 1981. The taxonomic implic-ations of genetic and environmentally induced variations in seaweed morphology. The Botanical Review, 47(3):313-347
    Noda M. 1971. Flora of Chinese northeastern region(Manchuria):I. Algae of the North Eastern China and Korea. Kazama study Co., Ltd., 1383-1601
    Patel R J. 1971. Cytotaxonomical studies on Chaetomorpha linum(OF. Müll.) Kütz. and C. aerea(Dillwyn) Kütz.. Phykos, 11:17-22
    Price W M. 1967. Some aspects of the biology and taxonomy of the unbranched Cladophorales in the British Islands[dissertation]. England:University of Liverpool
    Rosenvinge L K. 1893. Gr.nlands Havalger. Meddelser om Gr.nland, 3:763-981
    Silva P C, Basson P W, Moe R L. 1996. Catalogue of the Benthic Mar-ine Algae of the Indian Ocean. Botany:University of California Publications, 79:1-1259
    Sinha J P. 1958. Chromosome numbers and life cycles in members of Cladophorales. British Phycological Journal, 1:24-27
    Swofford D L. 2002. PAUP*. Phylogenetic analysis using parsimony(* and other methods). Sunderland, Massachusetts:Sinauer Asso-ciates
    Tamura K, Dudley J, Nei M, et al. 2007. MEGA4:Molecular evolution-ary genetics analysis(MEGA) Software Version 4. 0. Molecular Biology and Evolution, 24(8):1596-1599
    Thompson J D, Gibson T J, Plewniak F, et al. 1997. The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Re-search, 25(24):4876-4882
    Tseng C K. 1936. Studies on the marine Chlorophyceae from Hainan. Chinese Marine Biological Bulletin, 1:129-200
    Tseng C K. 1938. Studies on the marine Chlorophyceae from Hainan, Ⅱ. Lingn Sci J, 17(2):141-149
    Tseng C K. 1983. Common Seaweeds of China. Beijing:Science Press Tseng C K, Li L C. 1935. Some marine algae from Tsingtao and Che-foo, Shantung. Bulletin of the Fan Memorial Institute of Bio-logy(Botany), 6(4):183-235
    Womersley H B S. 1984. The Marine Benthic Flora of Southern Aus-tralia. Part I. Adelaide:Government Printer, South Australia, 1-329
    Womersley H B S, Bailey A. 1970. Marine algae of the Solomon Is-lands. Philosophical Transactions of the Royal Society of B:Bio-logical Sciences, 259(830):257-352
    Yoshida T. 1998. Marine Algae of Japan. Tokyo:Uchida Rokakuho Publishing Co., Ltd., 1-1222
    Zhou Z Y, Chen Z H. 1983. A list of marine algae from Fujian coast. Journal of Oceanography in Taiwan Strait(in Chinese), 2(2):91-102
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