ZHU Yaoyao, SUN Chengjun, SONG Yingfei, JIANG Fenghua, YIN Xiaofei, TANG Min, DING Haibing. The study of the adductor muscle-shell interface structure in three Mollusc species[J]. Acta Oceanologica Sinica, 2016, 35(8): 57-64. doi: 10.1007/s13131-016-0878-x
Citation: ZHU Yaoyao, SUN Chengjun, SONG Yingfei, JIANG Fenghua, YIN Xiaofei, TANG Min, DING Haibing. The study of the adductor muscle-shell interface structure in three Mollusc species[J]. Acta Oceanologica Sinica, 2016, 35(8): 57-64. doi: 10.1007/s13131-016-0878-x

The study of the adductor muscle-shell interface structure in three Mollusc species

doi: 10.1007/s13131-016-0878-x
  • Received Date: 2015-05-11
  • Rev Recd Date: 2015-09-14
  • The adductor muscle scar (AMS) is the fixation point of adductor muscle to the shell. It is an important organicinorganic interface and stress distribution area. Despite recent advances, our understanding of the structure and composition of the AMS remain limited. Here, we report study on the AMS of three bivalves:Mytilus coruscus, Chlamys farreri and Ruditapes philippinarum. Results showed that there were significant differences among their AMS structures. Both M. coruscus and C. farreri were found to have a columnar layer above the nacreous platelet shell structure at the AMS and this layer was more organized in M. coruscus. There was no distinguishable twolayer structure in R. philippinarum. Atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR) results showed that the AMS was much smoother than the nacreous inner shell in all the three species and the AMS had minor different compositions from the nacreous shell layer. SDS-PAGE (sodium dodecyl-sulfate polyacrylamide gel electophoresis) study of the proteins isolated from the interface indicated that there was a 70 kDa protein which seemed to be specifically located to the highly organized columnar AMS structure in Mytilus coruscus. Further analysis of this protein showed it contained high level of Asx (Asp+Asn), Glx (Glu+Gln) and Gly. The special structure and composition of the AMS might play important roles in the stability, adhesion and function at this stress distribution site.
  • loading
  • Addadi L, Joester D, Nudelman F, et al. 2006. Mollusk shell formation:a source of new concepts for understanding biomineralization processes. Chemistry-A European Journal, 12(4):980-987
    Andersen F A, Brečević L. 1991. Infrared spectra of amorphous and crystalline calcium carbonate. Acta Chemica Scandinavica, 45:1018-1024
    Balmain J, Hannoyer B, Lopez E. 1999. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analyses of mineral and organic matrix during heating of mother of pearl (nacre) from the shell of the mollusc Pinctada maxima. Journal of Bio-medical Materials Research, 48(5):749-754
    Belcher A M, Wu X H, Christensen R J, et al. 1996. Control of crystal phase switching and orientation by soluble mollusc-shell pro-teins. Nature, 381(6577):56-58
    Checa A. 2000. A new model for periostracum and shell formation in Unionidae (Bivalvia, Mollusca). Tissue and Cell, 32(5):405-416
    C.lfen H. 2010. Biomineralization:a crystal-clear view. Nature Ma-terials, 9(12):960-961
    Currey J D. 1977. Mechanical properties of mother of pearl in tension. Proceedings of the Royal Society B:Biological Sciences, 196(1125):443-463
    Currey J D. 1999. The design of mineralised hard tissues for their mechanical functions. Journal of Experimental Biology, 202(23):3285-3294
    Feng Q, Li H B, Pu G, et al. 2000. Crystallographic alignment of cal-cite prisms in the oblique prismatic layer of Mytilus edulis shell. Journal of Materials Science, 35(13):3337-3340
    Furuhashi T, Schwarzinger C, Miksik I, et al. 2009. Molluscan shell evolution with review of shell calcification hypothesis. Compar-ative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 154(3):351-371
    Kennedy V S, Newell R I, Eble A F. 1996. The Eastern Oyster:Crassostrea Virginica. Maryland:University of Maryland Sea Grant College
    Kong Y, Jing G, Yan Z, et al. 2009. Cloning and characterization of Prisilkin-39, a novel matrix protein serving a dual role in the prismatic layer formation from the oyster Pinctada fucata. Journal of Biological Chemistry, 284(16):10841-10854
    Lee S W, Jang Y N, Kim J C. 2011. Characteristics of the Aragonitic lay-er in adult oyster shells, Crassostrea gigas:structural study of myostracum including the adductor muscle scar. Evidence-Based Complementary and Alternative Medicine, 2011:742963
    Lin A, Meyers M A. 2005. Growth and structure in abalone shell. Ma-terials Science and Engineering:A, 390(1-2):27-41
    Lippmann F. 1973. Sedimentary Carbonate Minerals. Berlin Heidel-berg:Springer
    Lowenstam H A, Weiner S. 1989. On Biomineralization. Oxford:Ox-ford University Press
    Marie B, Le Roy N, Zanella-Cléon I, et al. 2011. Molecular evolution of mollusc shell proteins:insights from proteomic analysis of the edible mussel Mytilus. Journal of Molecular Evolution, 72(5-6):531-546
    Mount A S, Wheeler A, Paradkar R P, et al. 2004. Hemocyte-mediated shell mineralization in the eastern oyster. Science, 304(5668):297-300
    Song Y, Lu Y, Ding H, et al. 2013. Structural characteristics at the ad-ductor muscle and shell interface in Mussel. Applied Biochem-istry and Biotechnology, 171(5):1203-1211
    Spann N, Harper E M, Aldridge D C. 2010. The unusual mineral vater-ite in shells of the freshwater bivalve Corbicula fluminea from the UK. Naturwissenschaften, 97(8):743-751
    Suzuki M, Iwashima A, Tsutsui N, et al. 2011. Identification and char-acterisation of a calcium carbonate-binding protein, blue mus-sel shell protein (BMSP), from the nacreous layer. ChemBio-Chem, 12(16):2478-2487
    Suzuki M, Saruwatari K, Kogure T, et al. 2009. An acidic matrix pro-tein, Pif, is a key macromolecule for nacre formation. Science, 325(5946):1388-1390
    Takeuchi T, Endo K. 2006. Biphasic and dually coordinated expres-sion of the genes encoding major shell matrix proteins in the pearl oyster Pinctada fucata. Marine Biotechnology, 8(1):52-61
    Vagenas N V, Gatsouli A, Kontoyannis C G. 2003. Quantitative analys-is of synthetic calcium carbonate polymorphs using FT-IR spectroscopy. Talanta, 59(4):831-836
    Wainwright S A. 1969. Stress and design in bivalved mollusc shell. Nature, 224(5221):777-779
    Wheeler A P, Rusenko K W, Swift D M, et al. 1988. Regulation of in vitro and in vivo CaCO3 crystallization by fractions of oyster shell organic matrix. Marine Biology, 98(1):71-80
    Yoon G L, Kim B T, Kim B O, et al. 2003. Chemical-mechanical char-acteristics of crushed oyster-shell. Waste Management, 23(9):825-834
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (948) PDF downloads(897) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint