Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization

Zifeng Zhan; Kuidong Xu

doi:10.1007/s13131-020-1683-0

Volume 39 Issue 12

Jan. 2021

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Zifeng Zhan, Kuidong Xu. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization[J]. Acta Oceanologica Sinica, 2020, 39(12): 90-94. doi: 10.1007/s13131-020-1683-0

Citation:

Zifeng Zhan, Kuidong Xu. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization[J]. Acta Oceanologica Sinica, 2020, 39(12): 90-94. doi: 10.1007/s13131-020-1683-0

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Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization

doi: 10.1007/s13131-020-1683-0

Zifeng Zhan^{1, 2, 3},
Kuidong Xu^{1, 2, 3, 4
,
,}

1.
Laboratory of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
3.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
4.
University of Chinese Academy of Sciences, Beijing 100049, China

Funds: The National Natural Science Foundation of China under contract Nos 41876171 and 41406171.

More Information

Corresponding author: E-mail: kxu@qdio.ac.cn
Received Date: 2020-02-14
Accepted Date: 2020-07-14

Available Online: 2021-04-21

Publish Date: 2020-12-25

Abstract

Abstract

The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A. crassum infections on host molluscs.
- oligonucleotide probes,
- molecular methods,
- scuticociliates,
- SSU rRNA,
- abalone,
- mollusc

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References(16)

References

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