Study of screening, transport pathway, and vasodilation mechanisms on angiotensin-I converting enzyme inhibitory peptide from <i>Ulva prolifera</i> proteins

Zhiyong Li; Yuan He; Hongyan He; Caiwei Fu; Mengru Li; Aiming Lu; Dongren Zhang; Tuanjie Che; Songdong Shen

doi:10.1007/s13131-023-2170-1

Volume 42 Issue 11

Nov. 2023

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Article Navigation > Acta Oceanologica Sinica > 2023 > 42(11): 98-106

Zhiyong Li, Yuan He, Hongyan He, Caiwei Fu, Mengru Li, Aiming Lu, Dongren Zhang, Tuanjie Che, Songdong Shen. Study of screening, transport pathway, and vasodilation mechanisms on angiotensin-I converting enzyme inhibitory peptide from Ulva prolifera proteins[J]. Acta Oceanologica Sinica, 2023, 42(11): 98-106. doi: 10.1007/s13131-023-2170-1

Citation:

Zhiyong Li, Yuan He, Hongyan He, Caiwei Fu, Mengru Li, Aiming Lu, Dongren Zhang, Tuanjie Che, Songdong Shen. Study of screening, transport pathway, and vasodilation mechanisms on angiotensin-I converting enzyme inhibitory peptide from Ulva prolifera proteins[J]. Acta Oceanologica Sinica, 2023, 42(11): 98-106. doi: 10.1007/s13131-023-2170-1

Citation:

Zhiyong Li, Yuan He, Hongyan He, Caiwei Fu, Mengru Li, Aiming Lu, Dongren Zhang, Tuanjie Che, Songdong Shen. Study of screening, transport pathway, and vasodilation mechanisms on angiotensin-I converting enzyme inhibitory peptide from Ulva prolifera proteins[J]. Acta Oceanologica Sinica, 2023, 42(11): 98-106. doi: 10.1007/s13131-023-2170-1

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Study of screening, transport pathway, and vasodilation mechanisms on angiotensin-I converting enzyme inhibitory peptide from Ulva prolifera proteins

doi: 10.1007/s13131-023-2170-1

1.
School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215101, China
2.
Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province, Lanzhou 730030, China

More Information

Corresponding author: Songdong Shen Email: shensongdong@suda.edu.cn
Received Date: 2022-07-29
Accepted Date: 2023-01-11

Available Online: 2024-01-04

Publish Date: 2023-11-01

Abstract

Abstract

In this study, Ulva prolifera protein was used for preparing angiotensin-I converting enzyme (ACE)-inhibitory peptide via virtual gastrointestinal digestion and in silico screening. Some parameters of the obtained peptide, such as inhibition kinetics, docking mechanism, stability, transport pathway, were explored by Lineweaver-Burk plots, molecular docking, in vitro stimulate gastrointestinal (GI) digestion and Caco-2 cells monolayer model, respectively. Then, a novel anti-ACE peptide LDF (IC₅₀, (1.66 ± 0.34) μmol/L) was screened and synthesized by chemical synthesis. It was a no-competitive inhibitor and its anti-ACE inhibitory effect mainly attributable to four Conventional Hydrogen Bonds and Zn701 interactions. It could keep activity during simulated GI digestion in vitro and was transported by peptide transporter PepT1 and passive-mediated mode. Besides, it could activate Endothelial nitric oxide synthase (eNOS) activity to promote the production of NO and reduce Endothelin-1 (ET-1) secretion induced by Angiotensin II (Ang II) in Human Umbilical Vein Endothelial Cells (HUVECs). Meanwhile, it could promote mice splenocytes proliferation in a concentration-dependent manner. Our study indicated that this peptide was a potential ingredient functioning on vasodilation and enhancing immunity.
- Ulva prolifera protein,
- ACE-inhibitory peptide,
- in vitro GI digestion,
- vasodilation mechanism,
- immunomodulation

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